Histone Lysine Trimethylation or Acetylation can be Modulated by Phytoestrogen, Estrogen or Anti-HDAC in Breast Cancer Cell Lines

Abstract

We demonstrated that soy phytoestrogens tend to modify transcription through the demethylation and acetylation of histones in breast cancer cell lines.

Endocrine disruption of the epigenome: a breast cancer link

“…A number of isoflavones have also been found to alter the histone methylation and acetylation signature in breast cancer cell lines (Jawaid et al 2010, Dagdemir et al 2013). Altered miRNA expression in response to isoflavones and resveratrol has been well demonstrated in cancers of the prostate, pancreas and ovaries (Li et al 2010, Sheth et al 2012, Chiyomaru et al 2013, Xu et al 2013); however, there has been limited work done to elucidate miRNA regulation by phytoestrogens in the breast.…”

Molecular and Epigenetic Biomarkers in Luminal Androgen Receptor: A Triple Negative Breast Cancer Subtype

“…Therefore, TNBC subtype is anticipated to be a highly heterogeneous group; the molecular understanding of this breast cancer type is critical to develop innovative and effective drugs. Moreover, the subtyping of TNBC is essential to better identify molecular biomarkers, and epigenetic understanding can contribute to this challenge.In a previous study, we have observed a decrease in H3K27me3 and H3K9me3 markers with BRCA1, EZH2, and ER-a genes in tumor breast cell lines (MDA-MB-231 and MCF7) treated with 17b-estradiol and soybean phytoestrogens and an increase in H4K8ac and H3K4ac markers with these genes following suberoylanilide hydroxamic acid (SAHA) (Dagdemir et al, 2013). These epigenetic modifications have responded to different treatments and have been able to mediate silencing or enhancing genes.…”

“…In a previous study, we have observed a decrease in H3K27me3 and H3K9me3 markers with BRCA1, EZH2, and ER-a genes in tumor breast cell lines (MDA-MB-231 and MCF7) treated with 17b-estradiol and soybean phytoestrogens and an increase in H4K8ac and H3K4ac markers with these genes following suberoylanilide hydroxamic acid (SAHA) (Dagdemir et al, 2013). These epigenetic modifications have responded to different treatments and have been able to mediate silencing or enhancing genes.…”

“…1B). AR mRNAs were evaluated by reverse transcriptase (RT)-quantitative polymerase chain reaction (Q-PCR) (Ngollo et al, 2014) as shown in Figure 2A and were found highly expressed in the LAR subtype compared with the basal-like subtypes with a significant difference ( p = 0.029).Therefore, H3 modifications on AR promoter gene were studied in LAR and basal-like subtypes by immunoprecipitation of chromatin (Dagdemir et al, 2013;Ngollo et al, 2014) following by Q-PCR (Fig. 2B).…”

“…Therefore, H3 modifications on AR promoter gene were studied in LAR and basal-like subtypes by immunoprecipitation of chromatin (Dagdemir et al, 2013;Ngollo et al, 2014) following by Q-PCR (Fig. 2B).…”