Histone variants: the artists of eukaryotic chromatin

Li, Min; Fang, Yuda

doi:10.1007/s11427-015-4817-4

Cited by 17 publications

(6 citation statements)

References 124 publications

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“…In addition, in nucleosomes canonical histones can be replaced by histone variants with the aid of histone chaperones. Histone variants diverge from canonical histones for a different amino acid sequence and, being subjected to specific PTMs, are able to alter the chromatin structure, influencing the nucleosome dynamics and consequently the transcriptional regulation (Li and Fang, 2015). Finally, PTMs can also collaborate with DNA methylation processes establishing regulatory loops that allow specific chromatin states to be reinforced, as demonstrated for CHG and CHH methylation and H3K9me2 modification (see above; Du et al, 2012; Jiang and Berger, 2017).…”

Section: Epigenetic Control In Plant/fruit Developmentmentioning

confidence: 99%

Rosaceae Fruit Development, Ripening and Post-harvest: An Epigenetic Perspective

et al. 2017

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Rosaceae is a family with an extraordinary spectrum of fruit types, including fleshy peach, apple, and strawberry that provide unique contributions to a healthy diet for consumers, and represent an excellent model for studying fruit patterning and development. In recent years, many efforts have been made to unravel regulatory mechanism underlying the hormonal, transcriptomic, proteomic and metabolomic changes occurring during Rosaceae fruit development. More recently, several studies on fleshy (tomato) and dry (Arabidopsis) fruit model have contributed to a better understanding of epigenetic mechanisms underlying important heritable crop traits, such as ripening and stress response. In this context and summing up the results obtained so far, this review aims to collect the available information on epigenetic mechanisms that may provide an additional level in gene transcription regulation, thus influencing and driving the entire Rosaceae fruit developmental process. The whole body of information suggests that Rosaceae fruit could become also a model for studying the epigenetic basis of economically important phenotypes, allowing for their more efficient exploitation in plant breeding.

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Section: Epigenetic Control In Plant/fruit Developmentmentioning

confidence: 99%

Rosaceae Fruit Development, Ripening and Post-harvest: An Epigenetic Perspective

et al. 2017

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“…4,[6][7][8] The preservation of the phenotype of eukaryotic cells depends closely on the structural conservation and the dynamic regulation of chromatin, the main nuclear DNA packaging structure. 9,10 Chromosomal information of mother cells is duplicated and segregated to its daughter cells in a highly regulated and complex process known as mitosis. 11,12 Defects in the distribution of this genetic information are associated with the development of cancer.…”

Section: Introductionmentioning

confidence: 99%

KDM4C Activity Modulates Cell Proliferation and Chromosome Segregation in Triple-Negative Breast Cancer

García

Lizcano

2016

Breast Cancer�(Auckl)

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The Jumonji-containing domain protein, KDM4C, is a histone demethylase associated with the development of several forms of human cancer. However, its specific function in the viability of tumoral lineages is yet to be determined. This work investigates the importance of KDM4C activity in cell proliferation and chromosome segregation of three triple-negative breast cancer cell lines using a specific demethylase inhibitor. Immunofluorescence assays show that KDM4C is recruited to mitotic chromosomes and that the modulation of its activity increases the number of mitotic segregation errors. However, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) cell proliferation assays demonstrate that the demethylase activity is required for cell viability. These results suggest that the histone demethylase activity of KDM4C is essential for breast cancer progression given its role in the maintenance of chromosomal stability and cell growth, thus highlighting it as a potential therapeutic target.

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“…Histones are therefore subject to a variety of regulatory processes, such as posttranslational modifications and regulated incorporation or removal at various genomic regions (Rando and Winston 2012). Chromatin is also regulated by the deposition of his-tone variants, which can alter chromatin structure and accessibility to regulate cellular processes (Li and Fang 2015). One conserved histone is the H3 variant CENP-A, which localizes to centromeres to mediate their epigenetic propagation and to serve as the foundation for kinetochore assembly (Palmer et al 1987;Van Hooser et al 2001;Black and Cleveland 2011;Verdaasdonk and Bloom 2011).…”

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confidence: 99%

Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant

et al. 2017

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The incorporation of histone variants into nucleosomes can alter chromatin-based processes. CENP-A is the histone H3 variant found exclusively at centromeres that serves as an epigenetic mark for centromere identity and is required for kinetochore assembly. CENP-A mislocalization to ectopic sites appears to contribute to genomic instability, transcriptional misregulation, and tumorigenesis, so mechanisms exist to ensure its exclusive localization to centromeres. One conserved process is proteolysis, which is mediated by the Psh1 E3 ubiquitin ligase in Saccharomyces cerevisiae (budding yeast). To determine whether there are features of the CENP-A nucleosome that facilitate proteolysis, we performed a genetic screen to identify histone H4 residues that regulate CENP-A degradation. We found that H4-R36 is a key residue that promotes the interaction between CENP-A and Psh1 Consistent with this, CENP-A protein levels are stabilized in H4-R36A mutant cells and CENP-A is enriched in the euchromatin. We propose that the defects in CENP-A proteolysis may be related to changes in Psh1 localization, as Psh1 becomes enriched at some 3' intergenic regions in H4-R36A mutant cells. Together, these data reveal a key residue in histone H4 that is important for efficient CENP-A degradation, likely by facilitating the interaction between Psh1 and CENP-A.

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Histone variants: the artists of eukaryotic chromatin

Cited by 17 publications

References 124 publications

Rosaceae Fruit Development, Ripening and Post-harvest: An Epigenetic Perspective

Rosaceae Fruit Development, Ripening and Post-harvest: An Epigenetic Perspective

KDM4C Activity Modulates Cell Proliferation and Chromosome Segregation in Triple-Negative Breast Cancer

Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant

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