Histopathologic Evaluation of Ecballium Elaterium Applied Nasal                     Mucosa in Rat Rhinosinusitis Model

Eti, Can Mehmet; Vayısoğlu, Yusuf; Kardaş, Berkan; Arpacı, Rabia Bozdoğan; Horasan, Elif Şahin; Kanık, Arzu; Eti, Neslihan; Yalın, Serap; Talaş, Derya Ümit

doi:10.1177/014556131809700616

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…Fresh raw juice of the E. elaterium plant is therapeutically used for rhinosinusitis and jaundice (Eti et al, 2018; Greige‐Gerges et al, 2007). The main reason for using this plant in traditional medicine has been suggested to be main active ingredient of the plant, cucurbitacins.…”

Section: Discussionmentioning

confidence: 99%

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Üremiş

Uremis

Çiğremiş

et al. 2022

Journal of Food Biochemistry

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Hepatocellular carcinoma is a common cancer type, especially among men. Although cucurbitacin I (CuI), widely found in plants belonging to the Ecballium elaterium (E. L) plant family, has been shown to have antitumorigenic properties in many cancer types, its anticancer effect, molecular mechanism, and apoptotic effect mediated by signal pathways on hepatocellular carcinoma have not been fully clarified. In the present study, we investigated the anticancer effect of CuI treated at different doses on the HepG2 cell line and the underlying mechanism in vitro. High-purity CuI was obtained from the E. elaterium plant with the aid of HPLC. The effects of this substance on the viability of cells were studied by the MTT assay. The effects of CuI on cell cycle progression and apoptosis were studied with flow cytometry. DNA breaks were analyzed by the Comet assay method.The proteins and genes involved in the JAK/STAT3, MAPK/ERK, and AKT/mTOR signaling pathways were investigated using Western blot and qRT-PCR, respectively. The results of this study demonstrated that CuI significantly reduced HepG2 cell growth in vitro, induced antiproliferation, and G2/M phase of the cell cycle was interrupted. Practical applicationsCuI administration was shown to downregulate the levels of proteins in the PI3K/ AKT/mTOR, MAPK, and JAK2/STAT3 cascades in HepG2 cells. CuI also reduced the expression of MAPK, STAT3, mTOR, JAK2, and Akt genes in different concentrations.DNA breaks are formed as a result of this effect. CuI, by reducing cell proliferation and promoting apoptosis, was found to have potential as a chemotherapeutic agent of hepatocellular carcinoma.

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Section: Discussionmentioning

confidence: 99%

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Üremiş

Uremis

Çiğremiş

et al. 2022

Journal of Food Biochemistry

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Stapes surgery leads to significant improvement in quality of life, independently from the surgical method: evaluation of stapes surgery using different prostheses and different quality of life measurements

Weiss

Schuldt

Großmann

et al. 2019

Eur Arch Otorhinolaryngol

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Ecballium elaterium extract reduces fibrosis during wound healing in rats

İbiloğlu

Alabalık

Keleş

et al. 2021

Biotechnology & Biotechnological Equipment

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We explored the effects of subcutaneously injected Ecballium elaterium (L) A. Rich. (EE) extract on skin wound-healing in rats, as well as the effects on the liver and pancreas. Twenty-eight rats were divided into two groups of 14 each: a saline control group (S) and an EE group. Both groups were divided into two subgroups according to the day of sacrifice (S-7 and S-14, and EE-7 and EE-14). All animals received 2.5 cm skin incisions followed by subcutaneous injections of either saline or 2.5 mg/kg EE per margin (5 mg/kg in total). The S and EE groups were compared in terms of the severity and type of local and neighbouring inflammation, vascularization, fibrosis and effects on the liver and pancreas. In addition, apoptosis and vascularity between S and EE groups were compared immunohistochemically with caspase-3 and cd-34 antibodies. There was no significant difference between the staining rates of caspase-3 and cd-34 in the immunohistochemical assay between the S and E groups. Subcutaneous EE was not toxic to the pancreas or liver; the EE-14 group exhibited less fibrosis than the S-14 group. Therefore, it can contribute to the proper closure of the lesion by reducing fibrosis during wound healing.

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Histopathologic Evaluation of Ecballium Elaterium Applied Nasal Mucosa in Rat Rhinosinusitis Model

Cited by 5 publications

References 10 publications

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Stapes surgery leads to significant improvement in quality of life, independently from the surgical method: evaluation of stapes surgery using different prostheses and different quality of life measurements

Ecballium elaterium extract reduces fibrosis during wound healing in rats

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