Histopathological aberrations and oxidative stress responses in Nile tilapia Oreochromis niloticus as influenced by dietary florfenicol and its metabolites

Biological Responses of Nile tilapia Oreochromis niloticus as Influenced by Dietary Florfenicol

“…The observation of excessive glycogen-type vacuolation in the liver could be attributed to glycogenesis. There is compelling evidence that excess stress might lead to hepatic disorders, which greatly reduce the food consumption and growth performance of fish [ 6 , 26 , 27 ], similarly to the findings of the present study. Among several metabolic disturbances, glycogenic hepatopathy (hepatomegaly) and cytoplasmic vacuolation were the most common ones observed in our study.…”

“…With the termination of dosing, the juveniles showed no abnormalities and increased interest in feeding, though insignificantly, similarly to a previous study [ 24 ]. No mortalities were noted in the 1× group, as was also observed in earlier studies at therapeutic or lower FFC dose levels [ 6 , 25 , 26 ]. At 10 days of dosing we observed significantly increased glucose levels in both groups, indicating that FFC prompted stress even at the therapeutic dose and gratified the extra demands of fish for metabolic energy under stressful conditions, similarly to the results of prior studies [ 6 , 26 , 27 ].…”

“…No mortalities were noted in the 1× group, as was also observed in earlier studies at therapeutic or lower FFC dose levels [ 6 , 25 , 26 ]. At 10 days of dosing we observed significantly increased glucose levels in both groups, indicating that FFC prompted stress even at the therapeutic dose and gratified the extra demands of fish for metabolic energy under stressful conditions, similarly to the results of prior studies [ 6 , 26 , 27 ]. In the 1× group, the levels remained constant until day 14 PFD, signifying the persistence of stress.…”

“…Erythrocytic morphological alterations often change the intracellular Hb content [ 42 ] and our results in relation to Hb supported their findings. The increased erythrocyte malformation may be related to increased lipid peroxidation in fish tissues and oxidative stress, as was observed in earlier studies [ 26 , 43 ]. The presence of more cytosol projections could be attributed to the lipid membrane’s greater permeability and flexibility as a result of this accelerated peroxidation.…”

“…The ALP levels did not decrease with the cessation of dosing, suggesting persisting liver inflammation in the dosed fish. The role of oxidative damage in the liver tissues and cell necrosis in response to FFC toxicity [ 26 ] could not be ruled out.…”