Histopathological and Biochemical Effect of Vitamin C and D on Phosphine-Induced Hepatotoxicity in Wistar Rats

Abstract

Background: Aluminum phosphide is becoming a very common agent for self-poisoning. The lack of specific antidote for phosphide poisoning has aroused the current interest of research. Objectives: This study aimed to investigate the possible protective effect of Vitamin C and D in phosphine-induced hepatotoxicity in Wistar rats. Methods: In this experimental study, 20 Wistar rats were randomly divided into five groups ( n = 12). Group I was apparently normal Control Group. Groups II-V were induced with 2.5 mg/kg ALP. Groups II was without further treatment while Groups III-IV received vitamin C at 100mg/kg/bw and vitamin D at 10mg/kg/bw. Group V was co-administered with vitamin C at 100mg/kg/bw and vitamin at 10mg/kg/bw. The treatments lasted for 28 days. Results: The results showed that Vitamin C and D (P<0.05) increased antioxidant capacity. Combined active constituents of the Vitamin C and Vitamin D raised the GPX antioxidant activities when compared to the phosphine poisoned group treated individually with Vitamin C and D. The combined activities of Vitamin C and Vitamin D showed the highest antioxidant effect as compared with the control Conclusion: The combined activities of Vitamin C and D supplementation improved the antioxidant defense system and histology of the liver in phosphine poisoning. Keywords: Metal phosphide, Carotene, Liver cell damage, Poison

Ascorbate and Alpha-Tocopherol Mitigate Toxic Pathological Changes in Adult Wistar Rats Exposed to Cypermethrin

“…Food contaminated with insecticides, such as cypermethrin, has an increased risk of harming both humans and domesticated animals. The prophylactic use of vitamins as a preventive measure in medical intervention for pathological conditions can be beneficial due to their ability to mitigate oxidative stress induced by cypermethrin exposure (Oladele et al 2022, Akinpelu et al 2023. Overall, this study underscores the detrimental effects of cypermethrin on the cardiopulmonary system.…”

“…However, the unregulated use of pyrethroids potentially induces oxidative stress and cardiopulmonary toxicity when food and water sources for human consumption become contaminated (Shaffo et pesticide for farming. The dose for the administration of cypermethrin was determined by a thorough analysis of multiple prior studies (Abdus Sallam et al 2020, Akinpelu et al 2023.…”

“…Studies have suggested that vitamins can act as a form of therapy to tackle oxidative stress. Ascorbate and alpha-tocopherol are among these beneficial vitamins (Pisoschi & Pop 2015, Wang & Dong 2018, Akinpelu et al 2023. Oxidative stress arises from an imbalance between the presence of oxidants in the body and the body's ability to combat them.…”

“…The stained sections were examined using a binocular light microscope (model XSZ-107BN, No. 071771, Olympus, Japan), while micrographs were taken at 100X magnification with a digital camera (model Easyshare C183, Kodak, USA) (Suvarna et al 2019, Akinpelu et al 2023. The collected data were subjected to statistical analysis using IBM SPSS Statistics for Windows, version 25.0 (IBM Corp., Armonk, N.Y., USA).…”

Enhancing pregnancy outcomes: the role of antioxidants in obstetrics

“…Numerous pregnancy issues have been linked to oxidative stress, which is caused by an imbalance between the body's antioxidant defense mechanisms and reactive oxygen species (ROS). Because it serves as a crucial interface between the mother and the fetus, the placenta is especially vulnerable to oxidative damage, which could compromise its functions and have an adverse influence on fetal development (11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”