Histopathological and biochemical evidence of hepatopancreatic toxicity caused by cadmium in white shrimp, Palaemonetes argentinus

Boudet, L.N. Chiodi; Polizzi, P.; Romero, María Belén; Robles, Alicia; Marcovecchio, Jorge Eduardo; Gerpe, Marcela Silvia

doi:10.1016/j.ecoenv.2014.11.019

Cited by 59 publications

(14 citation statements)

References 47 publications

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“…The TEM studies showed that Chlorpyrifos produce severe degeneration of cellular organelles including deformation of the apical surface of absorptive cell with dispersed microvilli, mitochondrial swelling with ballooned cristae, deformed cisternae of RER, lytic and vacuolated cytoplasm and pyknosis of nuclei. This finding is in agreement with Abd El-Atti (2002) and Chiodiboudet et al (2015) who found that Mercury and Cadmium induced destructive effects on the ultrastructure of the hepatopancreas of P. clarkii and Palaemonetes argentines, respectively. Desouky et al (2013) revealed severe pathological changes in hepatopancreatic cells of P. clarkia after exposure to Ethionincluding vacuolation, degeneration and distinct cellular damage; these histopathological alterations may be responsible for the high toxicity of Ethion to the crayfish.…”

Section: Discussionsupporting

confidence: 92%

Effect of Chlorpyrifos and Neem seed extract (Azadirechtein) on hepatopancreatic cellular structures of the freshwater crayfish Procambarus clarkii

Zeinab¹,

menam²,

Radwa³

et al. 2015

Egypt. J. of Aquatic Biolo. and Fish.

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Histological and ultrastructural studies on the normal structures of hepatopancreas of P. clarkii showed three main cell types, secretory, absorptive and fibrillar cells. The ultrastructural alterations in the hepatopancreas of the red swamp crayfish P. clarkii exposed to sublethal concentrations of Chlorprifos and neem seed extract (Azadirechtein) pesticides were studied. LC 25 of Chlorpyrifos (0.0075 ppm) produced deformation of the apical surface of absorptive cell, dispersedmicrovilli, mitochondrial swelling with ballooned cristae, deformed cisternae of RER, lytic and vacuolated cytoplasm and pyknosis of nuclei. On the other hand, LC 25 of Azadirechtein (4.99 ppm) caused deformation of mitochondria with the sparce of their cisternae, fragmentation of microvilli, ruptured RER beside the presence of autophagic vacuole containing dark granules and finally bizzar nuclei having segregated nucleoli and irregular clumps of their chromatin. Chlorpyrifos was found to be more toxic than Azadirechtein on P. clarkii.

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Section: Discussionsupporting

confidence: 92%

Effect of Chlorpyrifos and Neem seed extract (Azadirechtein) on hepatopancreatic cellular structures of the freshwater crayfish Procambarus clarkii

Zeinab¹,

menam²,

Radwa³

et al. 2015

Egypt. J. of Aquatic Biolo. and Fish.

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“…This capacity was observed after cessation of exposure by Chiodi-Boudet et al (2015) in Palaemonetes argentinus, and the energetic demand of this process is likely to depend on the degree of histological damage. This was more evident in the case of the Cd + Zn mixture, which might explain the progressively slower growth and smaller sizes observed with increasing concentrations of this mixture.…”

Section: Discussionmentioning

confidence: 99%

“…This accumulation is likely to cause structural damages, such as the severe alterations described by Chiodi-Boudet et al (2015) in the freshwater shrimp Palaemonetes argentinus exposed during 15 days to a Cd concentration equivalent to 50% of the LC50-96h for that species. However, our result obtained with Cd levels equivalent to approximately 10% of the Wu, et al (2008), while exposure during 21 days to a higher concentration (400 µg L -1 ) resulted in highly vacuolized epithelial cells, cellular hypertrophy and tubular necrosis.…”

Section: Discussionmentioning

confidence: 99%

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Frías-Espericueta¹,

Abad‐Rosales²,

Vargas-Jimenez³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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Litopenaeus vannamei postlarvae were exposed during 4 weeks to progressively increasing sublethal concentrations of cadmium and zinc (Cd: 8.8, 24, Zn: 81, 160, ), used alone or in mixture, in order to evaluate their joint effect on survival and growth, and on the histological structure of hepatopancreas and gills of the exposed organisms. After exposure to single metals there were no differences between control and treatments in survival and growth, while survival was significantly lower with the highest concentration of the mixture. Growth was also affected and showed a significant dose-related response. No histopathologies were observed in gills of shrimps exposed to single metals, while their hepatopancreas showed moderate to severe changes during the first three weeks. With the mixture, moderate edemas were present in gills lacunae and the hepatopancreas had sloughing of epithelial cells until the third week, but no histological alterations were evident by the end of the experiment. The results of this study show a synergistic effect of the mixture Cd + Zn on survival, growth and histological structure of hepatopancreas and gills of the Pacific white shrimp L. vannamei.

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“…In the decapod HP, haemocytic infiltration has been reported as initial response of the immune system to tissue damage (traumatic or due to infection), starvation, toxicity and environmental stress (Johnson, ; McGallery 2011; Chiodi Boudet et al., ; Longo & Diaz, ). In the present study, starvation induced the activation of the inflammatory pathway, stimulating the migration of haemocytes to the intertubular spaces of the HP.…”

Section: Discussionmentioning

confidence: 99%

Effect of starvation and refeeding on the hepatopancreas of whiteleg shrimp Penaeus vannamei (Boone) using computer‐assisted image analysis

Cervellione

McGurk

Eriksen

et al. 2017

Journal of Fish Diseases

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Under normal farming conditions, shrimp can experience starvation periods attributable to disease outbreaks or adverse environmental conditions. Starvation leads to significant morphological changes in the hepatopancreas (HP), being the main organ for absorption and storage of nutrients. In the literature, limited research has described the effect on the HP of periods of starvation followed by refeeding and none in whiteleg shrimp (Penaeus vannamei) using computer-assisted image analysis (CAIA). This study describes the effect of starvation and starvation followed by refeeding on the HP of whiteleg shrimp using CAIA. Visiopharm software was used to quantify the following morphological parameters, measured as ratio to the total tissue area (TLA): total lumen area (TLA:TTA), haemocytic infiltration area in the intertubular spaces (HIA:TTA), B-cell vacuole area (VBA:TTA), lipid droplet area within R cells (LDA:TTA) and F-cell area (FCA:TTA). Significant changes were measured for HIA:TTA and LDA:TTA during starvation (increase in HIA:TTA associated with decrease in LDA:TTA) and starvation followed by refeeding (decrease in HIA:TTA associated with increase in LDA:TTA). In the future, HIA:TTA and LDA:TTA have the potential to be used in a pre-emptive manner to monitor the health of the HP, facilitate early diagnosis of diseases and study the pathophysiology of the organ.

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Histopathological and biochemical evidence of hepatopancreatic toxicity caused by cadmium in white shrimp, Palaemonetes argentinus

Cited by 59 publications

References 47 publications

Effect of Chlorpyrifos and Neem seed extract (Azadirechtein) on hepatopancreatic cellular structures of the freshwater crayfish Procambarus clarkii

Effect of Chlorpyrifos and Neem seed extract (Azadirechtein) on hepatopancreatic cellular structures of the freshwater crayfish Procambarus clarkii

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Effect of starvation and refeeding on the hepatopancreas of whiteleg shrimp Penaeus vannamei (Boone) using computer‐assisted image analysis

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