Histopathological and Histomorphometric analysis of Pancreas and liver of diabetic rats treated with MucunaPruriens seed extract.

Abstract

Background: Medicinal plants play a major role in controlling diabetes by producing microstructural changes in liver and pancreas. Quantification of islets and hepatocytes by using histomorphometric tools are effective in evaluating the antidiabetic action of drugs. Aims and Objectives:To assess the anti diabetic properties of alcoholic extracts of Mucuna Pruriens seeds (200 mg/kg b.w) by means of histological and histomorphometric analysis of pancreas and liver .Methods: Seventy two neonatal wistar rats were randomly divided into four groups, i.e. Normal , Diabetic control, diabetic rats treated with Mucuna and Glibenclamide. Diabetes was induced by a single intraperitoneal administration of streptozotocin (65mg/kg b.w) in 5 day old neonatal rats (n =54).12 weeks after the injection, animals were divided in to four groups with 18 animals in each. The study groups were as follows. Group A -Normal control, Group B -STZ untreated, Group C -Mucuna Pruriens 200mg/kg, Group D -Glibenclamide (1mg/kg). Fasting blood sugar levels were monitored once a week during the drug study .i.e. 12 weeks. At the end of every 4th week, 6 animals from each group were sacrificed for histological and histomorphometric studies on liver and Pancreas. Results:From the histological studies, the diabetic group showed reduction of cell population in the pancreas and depletion of the number of hepatocytes in the liver. After 12 weeks of Drug treatment, These changes were markedly reduced in Mucuna Pruriens treated group. The obtained results were further quantified by means of Histomorphometric analysis and the results showed reduction in the number and diameter of islets, number of beta cells and number of healthy hepatocytes in diabetic control group and treatment with Mucuna Pruriens seed extract significantly reversed these changes (p < 0.05)

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“…The typical sign of Non-Alcoholic Fatty Liver Disease (NAFLD) is vacuolations and inflammations 19 , which was obvious in the current study. Kidneys showed structural changes in the PCT and the Glomerulus with mononuclear infiltrations.…”

Biochemical and Histomorphometrical Analysis Of Hepatoprotective and Hypolipidemic Potential Of Combined Herbal Extract in Diabetic Rats

“…The number of healthy and necrotic hepatocytes were determined by direct counting method at 40X magnification using the ocular grid. The total number of healthy and necrotic hepatocytes was expressed as N/1000 μm 2 of the hepatic parenchyma (Rajesh et al, 2017).…”

Phytochemical Analysis and in Vitro DPPH Assay of Ethanolic Extract of Mucuna Pruriens Seed

“…Tannins are important phytochemical compound that possess antioxidant properties [19]. Flavonoids possess membrane stabilizing properties and inhibit lipid peroxidation in liver, brain, pancreas etc [20]. Phenols have antioxidant properties which carry out their protective activity on cells either by checking the free radical generation or by scavenging the free radicals [21].…”

“…Phenols have antioxidant properties which carry out their protective activity on cells either by checking the free radical generation or by scavenging the free radicals [21]. Various studies have reported the antioxidant and anti-inflammatory potentials of saponins [22,23]. Bioactive components like alkaloids and phytosterols are also considered effective in conditions like stress and depression [24].…”