Histopathological and Immunohistochemical Features of Natural Goat Scrapie

Sofianidis, George; Psychas, V.; Billinis, Charalambos; Spyrou, Vassiliki; Argyroudis, S.; Papaioannou, Nikolaos; Vlemmas, I.

doi:10.1016/j.jcpa.2006.06.004

Cited by 18 publications

(26 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scrapie in sheep was the first disease to be recognized within the group of the transmissible spongiform encephalopathies (TSE), which affect several mammalian species, including man and cattle. Naturally-occurring scrapie in goats was first reported in France [5], and although this and other subsequent descriptions [3,16,34] record contact with scrapie-infected sheep, some others do not [15,29,34]. In most cases, the disease in goats has been reported to closely resemble that in sheep, although differences in clinical manifestation between the two species have been described [4].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect ofPRNPgenotype and age

González¹,

Martin²,

Sisó³

et al. 2009

Vet. Res.

View full text Add to dashboard Cite

-Following a severe outbreak of clinical scrapie in 2006-2007, a large dairy goat herd was culled and 200 animals were selected for post-mortem examinations in order to ascertain the prevalence of infection, the effect of age, breed and PRNP genotype on the susceptibility to scrapie, the tissue distribution of diseaseassociated PrP (PrP d ), and the comparative efficiency of different diagnostic methods. As determined by immunohistochemical (IHC) examinations with Bar224 PrP antibody, the prevalence of preclinical infection was very high (72/200; 36.0%), with most infected animals being positive for PrP d in lymphoreticular system (LRS) tissues (68/72; 94.4%) compared to those that were positive in brain samples (38/72; 52.8%). The retropharyngeal lymph node and the palatine tonsil showed the highest frequency of PrP d accumulation (87.3% and 84.5%, respectively), while the recto-anal mucosa-associated lymphoid tissue (RAMALT) was positive in only 30 (41.7%) of the infected goats. However, the efficiency of rectal and palatine tonsil biopsies taken shortly before necropsy was similar. The probability of brain and RAMALT being positive directly correlated with the spread of PrP d within the LRS. The prevalence of infection was influenced by PRNP genetics at codon 142 and by the age of the goats: methionine carriers older than 60 months showed a much lower prevalence of infection (12/78; 15.4%) than those younger than 60 months (20/42; 47.6%); these last showed prevalence values similar to isoleucine homozygotes of any age (40/80; 50.0%). Two of seven goats with definite signs of scrapie were negative for PrP d in brain but positive in LRS tissues, and one goat showed biochemical and IHC features of PrP d different from all other infected goats. The results of this study have implications for surveillance and control policies for scrapie in goats.scrapie / goat / prion disease / transmissible spongiform encephalopathy

show abstract

Section: Introductionmentioning

confidence: 99%

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect ofPRNPgenotype and age

González¹,

Martin²,

Sisó³

et al. 2009

Vet. Res.

View full text Add to dashboard Cite

show abstract

“…1) and Greece. 25 The mean scores between the UK and Italian studies did not differ by more than approximately 1 score value at any given neuroanatomical location, with the exception of 2 neuroanatomical nuclei, the nucleus accumbens (CNS code 3) and the vestibular nuclei (CNS code 18) ( Table 2, Fig. 1).…”

Section: Vacuolar Profilingmentioning

confidence: 81%

“…17,25 In sheep, strong correlations were demonstrated between host PrP genotype and the distribution of different morphologic forms of PrP Sc within the medulla oblongata at the level of the obex. 26 No grouping of the UK goat cases was possible that might have suggested a clear influence of this or other host factors.…”

Section: Ihc Prp Sc Profilingmentioning

confidence: 99%

A Histopathologic and Immunohistochemical Review of Archived UK Caprine Scrapie Cases

et al. 2008

View full text Add to dashboard Cite

Abstract. In 2005, a prion disease identified in a goat from France was reported to be consistent with disease from the bovine spongiform encephalopathy (BSE) agent. Subsequent retrospective examination of UK goat scrapie cases led to the identification of one potentially similar, but as yet unconfirmed, case from Scotland. These findings strengthened concerns that small ruminant populations exposed to the BSE agent have become infected. The lack of data relating specifically to scrapie in goats has been contributory to past assumptions that, in general, sheep and goats respond similarly to prion infections. In this study, brain material from 22 archived caprine scrapie cases from the UK was reviewed by histopathology and by immunohistochemical examination for accumulations of disease-specific prion protein (PrP Sc ) to provide additional data on the lesions of caprine scrapie and to identify any BSE-like features. The vacuolar change observed in the goats was characteristic of transmissible spongiform encephalopathies in general. PrP Sc immunohistochemical morphologic forms described in scrapie and experimental BSE infections of sheep were demonstrable in the goats, but these were generally more extensive and variable in PrP Sc accumulation. None of the cases examined showed a PrP Sc immunohistochemical pattern indicative of BSE.

show abstract

“…This is especially concerning since goats can have more subtle scrapie symptoms than sheep [6,21,25]. Sheep PRNP genetic testing has greatly aided sheep scrapie …”

Section: Discussionmentioning

confidence: 99%

Prion gene (PRNP) haplotype variation in United States goat breeds(Open Access publication)

et al. 2008

View full text Add to dashboard Cite

-Scrapie eradication efforts cost 18 million dollars annually in the United States and rely heavily upon PRNP genotyping of sheep. Genetic resistance might reduce goat scrapie and limit the risk of goats serving as a scrapie reservoir, so PRNP coding sequences were examined from 446 goats of 10 breeds, 8 of which had not been previously examined at PRNP. The 10 observed alleles were all related to one of two central haplotypes by a single amino acid substitution. At least five of these alleles (M142, R143, S146, H154, and K222) have been associated with increased incubation time or decreased odds of scrapie. To the best of our knowledge, neither S146 nor K222 has been found in any goats with scrapie, though further evaluation will be required to demonstrate true resistance. S146 was more common, present in several breeds at widely varying frequencies, while K222 was observed only in two dairy breeds at low frequency. Overall, this study provides frequency data on PRNP alleles in US goats, shows the pattern of relationships between haplotypes, and demonstrates segregation of multiple scrapieassociated alleles in several breeds not examined before at PRNP. scrapie / goat / polymorphism / resistance / prion

show abstract

Histopathological and Immunohistochemical Features of Natural Goat Scrapie

Cited by 18 publications

References 44 publications

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect ofPRNPgenotype and age

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect ofPRNPgenotype and age

A Histopathologic and Immunohistochemical Review of Archived UK Caprine Scrapie Cases

Prion gene (PRNP) haplotype variation in United States goat breeds(Open Access publication)

Contact Info

Product

Resources

About