Histopathological Changes (Gills and Liver) and Clinical Signs of Common Carp, Cyprinus Carpio L. Exposed to Graphene Nanoparticles

Abstract

This study was conducted to investigate the effect of graphene nanoparticles on histopathological changes and clinical signs of common carp Cyprinus carpio. A total of 48 fish were used in the experiment with an average weight 68.44 g. Fish were exposed to graphene nanoparticles at concentrations of 10 and 20 mg / L for the T1 and T2 respectively, as well as to control group (without graphene nanoparticles). Fish exposed to graphene showed no abnormal behavior and no mortality satisfactory clinical signs in the experimental fish. Histopathological changes of gills showed fusion with shorting of the secondary lamellae, lifting up secondary lamellae beside necrosis and hyperplasia of the epithelial cells of secondary lamellae in gills. On the other hand, there was necrosis of hepatocytes, dilation of sinusoids, hydrobic degeneration, hepatocellular vacuolation, hypertrophy and hydrobic of degeneration in liver. The present study was suggested to investigate of the graphene nanoparticles effects on histopathological changes and clinical signs to clarify a clear representation about on fish and its danger of its accumulation in aquatic environment.

Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

“…Much higher levels of GO accumulation were evidenced in lower trophic level aquatic organisms with low potential for biomagnification . Despite a lack of evidence of acute toxicity in fish to date, clear sublethal effects associated with GBM exposure in multiple species including zebrafish, Danio rerio , − climbing perch, Anabas testudineus , common carp, Cyprinus carpio , geophagus, Geophagus iporangensis , and tilapia were reported, including pathological damage in tissues (e.g., liver, gills, intestine), metabolic disturbances, changes in oxidative stress parameters at enzymatic and genetic levels, inflammatory responses, effects on neurotransmission, as well as alterations in predator avoidance behavior. In addition, changes in the gut microbiota leading to a decrease in the abundance of beneficial bacteria and dysbiosis of bacterial community, was evidenced following chronic exposure (25 days) to GO at relatively high concentrations (0.05, 0.5, and 5 mg/L), and following a 7-day exposure to GO both at low and high doses (50 or 500 μg/L) .…”

Sub-chronic Effects of Zinc oxide Nanoparticles on Ovary, Gills, Kidney and Liver of Fresh Water Fish (Cyprinus carpio): histopathological Study

“…To ascertain how graphene NPs affect histological alterations, Al-Rudainy and Khalel [10] susceptible to environment threat analysis, and study of these combinations should be given top importance [11].…”

Carbon nanotubes as control agents against the Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae)

“…Nanotechnology is a term that involving a wide range of technologies dealing with structures and processes at the nanometer scale (Stadler et al, 2018). This principal is employed to enhance the efficacy or reduce the environmental contamination (Sabry & Ragaei, 2018;Al-Rudainy & Khalel, 2019). The application of synthetic nanomaterials has increased recently in different fields such as medicine, agriculture, and food conservation (Beyene et al, 2017;Vangijzegem et al, 2019).…”