Histopathological changes in dental pulp of rats following radiotherapy

Abstract

Background:Radiotherapy is one of the oral and pharyngeal cancer treatment methods that can cause damage to the tissues in the radiation area; the purpose of this study is to evaluate the effects of radiotherapy on dental pulp tissue in rats.Materials and Methods:In this interventional, experimental double-blind study, 30 rats were studied in three groups (n = 10 each). The first group received 12 gray (Gy), the second group received 18 Gy in one session, and the third group was not exposed to radiation (control group). The 5 μm sections of mandibular molar tooth were prepared and stained with hematoxylin and eosin. Samples were studied under optical microscope to evaluate and score inflammation, necrosis, hyalinization, and vascular congestion. The data were coded and analyzed by statistical tests of χ2 and Fisher's exact tests. The significant level of P = 0.05.Results:In Group 1, necrosis in two cases, inflammation in one case, hyalinization in one case, and vascular congestion in four cases were observed. In Group 2, inflammation in four cases, hyalinization in two cases, and vascular congestion in five cases were observed. In Group 3, inflammation was observed only in one case. In comparison between the groups, no significant differences were observed in inflammation (P > 0.05), necrosis (P > 0.05), and hyalinization (P > 0.05). However, the difference was significant for vascular congestion (P < 0.05).Conclusion:Radiotherapy with doses of 12 and 18 Gy had no significant effect on inflammation, necrosis, and hyalinization in all groups; however, the difference was significant for vascular congestion.

Protection from Radiation-induced Damage in Rat’s Ileum and Colon by Combined Regimens of Melatonin and Metformin: A Histopathological Study

“…For mucositis, grade 1 is including mild inflamed mucosa, grade 2 is associated with moderate erythema and ulceration, and grade 3 is associated with severe erythema and ulceration [ 16 ]. Congestion can score as normal= no congestion, grade 1= up to 3 congestive vessels, 2= 3 to 5 congestive vessels, and 3= more than 5 congestive vessels [ 17 ]. Degenerative changes scoring is as normal = 0.…”

Does irradiation of the tooth structure interfere with oxygen saturation measured by pulse oximetry?—An ex vivo laboratorial study

“…Furthermore, a clinical trial showed that mean SpO 2 increased after 6 months of radiation therapy compared to baseline values [11]. Therefore, the pulpal health of patients treated with ionising radiation should be carefully assessed [8].…”

“…In an experimental murine model, chemical alterations in pulp microcirculation and a sizable amount of inflammation, hyalinisation and vascular congestion were observed after exposure to ionising radiation to the dental pulp [8]. In addition, a systematic review of radiation-induced dental caries showed possible changes in the collagen of the dental pulp, causing secondary fibrosis and reduction in vascularisation and odontoblast metabolism [9].…”