Histopathological Changes in Joint Components during Contracture-A Study of the Long Term Knee Joint Immobility Model in the Rat-

Watanabe, Masanori; Hoso, Masahiro; Takemura, Keiju; Yoshikubo, Hiroaki; Matsuzaki, Taro; Kojima, Satoshi

doi:10.1589/rika.22.67

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…The cartilage appeared to be more or less confluent with the overlying connective tissue 20 ) . These findings are consistent with those reported in our previous study 21 , 22 ) . During the first two weeks of immobilization, ROM limitation caused damage in the myogenic component, and after two weeks, the arthrogenic component constituted more than 80% of the total restriction in ROM 20 ) .…”

Section: Discussionsupporting

confidence: 94%

“…Kirschner wires were fixed outside the wounds in a knee flexion posture with screw (4 mm diameter) and nuts. The instruments for external fixation weighed 6 to 7 g. The rat’s knee flexion angle (120 degrees) was determined by the method described in our previous studys 13 , 21 , 22 ) . This immobilization method is low cost, and immobilization can be reversed to perform exercise and then reestablished easily.…”

Section: Methodsmentioning

confidence: 99%

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Influence of ROM Exercise on the Joint Components during Immobilization

et al. 2013

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[Purpose] The aim of this study was to clarify the effects of the ROM exercise on joint components according to histopathological analysis. [Subjects and Methods] In total, twenty-six 9-week-old adult male Wistar rats were used in this study. The rats were randomly divided into three groups, the immobilization group (n=10), exercise group (n=10), and control group (n=6). The immobilization group and exercise group were anaesthetized and operated on under sterile conditions. The right knee joints in the immobilization group and exercise group were immobilized with external fixation at 120 degrees of flexion. Range of motion exercise was started from the day after immobilization. ROM exercise was performed in the exercise group once a day for 3 minutes, 6 days a week, for 2 weeks. [Result] The joint capsule in the immobilization group and exercise group showed narrowing of the collagen bundles in interstitial spaces but was less dense in the control group. In the immobilized group, a hyperplastic reaction was associated with infiltration into the articular cavity and adhesion to the surface of the articular cartilage. Conversely, in the exercise group, hyperplasia of tissue was localized to the synovial membrane. [Conclusion] This finding may suggest that ROM exercise induces some changes within the joint components and tissue metabolism.

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Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Influence of ROM Exercise on the Joint Components during Immobilization

et al. 2013

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“…In our previous study 12,13) in rats with immobilized hindlimb knee joints, the same findings were observed as in the present study, such as collagen fiber thickening and densification in the joint capsule, as well as decreased hindlimb knee joint range of motion, indicating that joint motion loss causes fibrosis and thickening of the joint capsule and may be a cause of joint contracture.…”

Section: Discussionsupporting

confidence: 91%

Age-related changes in rat joint capsule

Matsuzaki,

Takahashi,

Hoso

2023

J Phys Ther Sci

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We investigated morphological and histopathological changes in the joint capsule of rats with aging. [Materials and Methods] A total of 18 male Wistar rats were categorized into two groups: the control group (n=8), and the aged group (n=10). The aged group was reared until 75 weeks of age, while the control group was maintained until 11 weeks of age. At the end of the experiment period, the knee joints were sampled, joint capsules were subjected to histopathological analysis, and their thickness was measured. [Results] The joint capsule in the aging group exhibited significantly greater thickness compared to the control group. Histopathological examination revealed distinct differences between the two groups. The control group displayed gaps between the collagen fibers in the posterior joint capsule, along with loosely overlapping connective tissue and the presence of fat cells. Conversely, in the aged group's joint capsule, these gaps between the collagen fibers almost disappeared and fibers became densely packed and thickened. [Conclusion] These results were similar to our previous study in rats with immobilized hindlimb knee joints. Similar findings, including collagen fiber thickening, densification in the joint capsule, and reduced hindlimb knee joint range of motion, were consistent with those observed in the present investigation.

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“…Takemura et al [28] analyzed changes in the synovial membrane caused by anterior articular capsule broblasts, microvasodilation, and congestion due to plaster immobilization. Watanabe et al [29] and Matsuzaki et al [30] reported that intra-articular tissue adhesion does not completely cover the cruciate ligament in and around the knee, but originates from synovial brosis. The proliferation of intra-articular synovial tissue was responsible for the limited ROM found in the present study.…”

Section: Discussionmentioning

confidence: 99%

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

Zhou

Wang

et al. 2022

Preprint

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Background: The study aimed to elucidate the formation process and therapeutic strategies of knee extension contracture, we developed a novel rat model using an aluminum external fixator.Methods: Sixty male SD rats with mature bones were divided into the control group (n=6) and groups that had the left knee immobilized with an aluminum external fixator for 1, 2, and 3 days, and 1, 2, 3, 4, 6, and 8 weeks (n=6 in each group). The passive extension range of motion, histology, and expression of fibrosis-related proteins were compared between the control group and the immobilization groups.Results: Myogenic contracture progressed very quickly during the initial 2 weeks of immobilization; after 2 weeks, the contracture gradually changed from myogenic to arthrogenic. The arthrogenic contracture progressed slowly during the first week, rapidly progressed until the third week, and then showed a steady progression. Histological analysis confirmed that the anterior joint capsule of the extended fixed knee became increasingly thicker over time. Correspondingly, the level of TGFβ-1 in the anterior joint capsule also increased with the immobilization time. Over time, the cross-sectional area of muscle fibers gradually decreased, while the amount of intermuscular collagen and TGFβ-1 increased. Unexpectedly, the amount of intermuscular collagen and TGFβ-1 decreased during the late stage of immobilization (6–8 weeks).Conclusion: Myogenic contracture is stabilized after 2 weeks of immobilization, while arthrogenic contracture is stabilized after 3 weeks of immobilization. This novel rat model may be a useful tool to study the etiology of joint contracture and establish new therapeutic approaches.

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Histopathological Changes in Joint Components during Contracture-A Study of the Long Term Knee Joint Immobility Model in the Rat-

Cited by 10 publications

References 21 publications

Influence of ROM Exercise on the Joint Components during Immobilization

Influence of ROM Exercise on the Joint Components during Immobilization

Age-related changes in rat joint capsule

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

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