Histopathological changes of rat larynx mucosa with exposure to chronic thinner inhalation

Bolukbasi, Seyla; Habesoglu, Tulay Erden; Habeşoğlu, Mehmet; Samanci, Baver; Erol, Yesim; Gumrukcu, Gulistan; Egeli, Erol

doi:10.1016/j.otohns.2009.02.012

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…To qualitatively evaluate the severity of inflammation, hyperplasia of the epidermidis, amount of exudate on the epithelial surface, extent of thickening of the dermis, blood and lymph vessel dilation, and infiltration of polymorphonuclear leucocytes (PMNLs) and lymphocytes were taken into account. They were graded as +, mild inflammation (infiltration of few PMNLs and lymphocytes); ++, moderate inflammation (infiltration of some PMNLs and lymphocytes); and +++, severe inflammation (infiltration of many PMNLs and lymphocytes) 14 . Edema, vascular dilation, and pseudostratification were also graded as mild (+), moderate (++), or severe (+++).…”

Section: Methodsmentioning

confidence: 99%

“…They were graded as +, mild inflammation (infiltration of few PMNLs and lymphocytes); ++, moderate inflammation (infiltration of some PMNLs and lymphocytes); and +++, severe inflammation (infiltration of many PMNLs and lymphocytes). 14 Edema, vascular dilation, and pseudostratification were also graded as mild (+), moderate (++), or severe (+++). Cilia loss was evaluated morphologically by light microscopy.…”

Section: Pathologic Evaluationmentioning

confidence: 99%

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Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

Tatlipinar

Güneş

Özbeyli

et al. 2011

Otolaryngol.--head neck surg.

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Section: Methodsmentioning

confidence: 99%

Section: Pathologic Evaluationmentioning

confidence: 99%

Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

Tatlipinar

Güneş

Özbeyli

et al. 2011

Otolaryngol.--head neck surg.

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“…The use of light microscopy to determine the effects of various chemicals and treatments on the histomorphologic structure of nasal and sinus‐ciliated respiratory epithelium is well established in the literature 35–40. In addition, any changes in the histomorphologic structure of the paranasal sinus epithelium has correlated with functional sinus mucosal changes, such as a change in ciliary beat frequency 35–40. Furthermore, the human highly differentiated ciliated respiratory EpiAirway tissues used in this study are ideal for basic cell and molecular biology studies, pharmaceutical target validation, and preclinical toxicity/efficacy determination, as well as inhalation toxicity studies of occupational or environmental air pollutants and toxins 25.…”

Section: Discussionmentioning

confidence: 99%

The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa

et al. 2012

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Background Chronic recurrent sinusitis (CRS) is one of the most common chronic conditions in the United States. There is a significant subpopulation of CRS patients who remain resistant to cure despite rigorous treatment regimens including surgery, allergy therapy and prolonged antibiotic therapy. Antimicrobial photodynamic therapy (aPDT) is a non-invasive non-antibiotic broad spectrum antimicrobial treatment. Our previous in vitro studies demonstrated that aPDT reduced CRS polymicrobial biofilm and planktonic bacteria and fungi by >99.9% after a single treatment. Prior to human treatment however, aPDT treatment must be demonstrated to not result in histologic damage to the sinus ciliated respiratory epithelium. The objective of this study was to demonstrate the safety of aPDT treatment on a living human ciliated respiratory mucosal model (EpiAirway™). Methods A study of aPDT treatment of EpiAirway™ was performed. Treatment groups included a non-treatment control, laser light alone, photosensitizer alone and therapeutic photosensitizer and light combination (aPDT). At completion of treatment, the EpiAirway™ tissue was fixed in 10% formalin, paraffin-embedded, sectioned, H &E stained and mounted. All samples were blinded and microscopically examined by a human pathologist to assess any effect of aPDT on the tissue, cilia or mucosal glands. The results were correlated with the treatment parameters. Results The EpiAirway™ histologic study demonstrated no histologic alteration of the respiratory cilia or mucosal epithelium in any of the treatment groups. Conclusions aPDT is a safe treatment for CRS resulting in no histologic alteration of human ciliated respiratory mucosa as is found in the human sinuses.

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Chamomile flowers extract protects against thinner inhalation-induced lung toxicity via attenuating cytochrome P2E1 activity, surfactant deficiency, and alveolar structural injury in rats

Elgharieb,

El-Wakf,

Abdrabouh

2024

Beni-Suef Univ J Basic Appl Sci

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Background Lungs are adversely affected by repeated exposure to thinner fumes. This study aimed to examine the pulmonary toxic effects of chronic thinner inhalation and the possible protection by chamomile administration. Adult male Wistar rats were exposed to thinner fumes for 8 weeks (4 h/day, 6 days/week), while chamomile flower extract (400 mg/kg body weight) was given orally during thinner exposure for the same period. Results The study showed lung damage following chronic thinner exposure through increased cytochrome P2E1 (CYP2E1), superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde (MDA), with decreased antioxidant enzymes; superoxide dismutase (SOD), and catalase (CAT). Moreover, an elevation of lung enzymes; alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and lactate dehydrogenase (LDH) with depletion in total protein and albumin contents in serum and bronchoalveolar lavage fluid were observed. Thinner exposure also exhibited increased lung deoxyribonucleic acid (DNA) damage, transforming growth factor-β1 (TGF-β1), insulin-like growth factor-1 (IGF-1), hydroxyproline (HYP), and collagen type 1 (COL-1), with decreased serum surfactant protein-A (SP-A), total and differential leukocytes (WBCs) count, except for neutrophils. Histological investigations revealed deteriorative changes along with accumulated collagen fibers affecting the lung and other respiratory organs. Conclusion Supplementation of chamomile extract succeeded in preventing thinner-induced lung oxidative stress, enzyme leakage, surfactant deficiency, DNA damage, fibrosis, and histological injury. Therefore, consumption of chamomile extract could be recommended for alleviating thinner-induced health hazards and lung toxicity.

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Histopathological changes of rat larynx mucosa with exposure to chronic thinner inhalation

Abstract: On the basis of histopathological evaluations, it was shown that the harmful effect of inhalation of thinner in high concentrations to larynx mucosa is similar to the effect on other organs of the respiratory system.

Cited by 4 publications

References 11 publications

Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa

Chamomile flowers extract protects against thinner inhalation-induced lung toxicity via attenuating cytochrome P2E1 activity, surfactant deficiency, and alveolar structural injury in rats

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