Histopathological Effects of Aloe barbadensis and Soybean Oil on Rat Liver

Abstract

Barbadensis, which is a species of Aloe vera, is a popular plant used by the common people and in alternative medicine. This study aimed to analyze the effects of Aloe Barbadensis and soybean oil on liver. For this study Wistar Albino female rats were taken and divided into 3 equal groups; the first group was the control group wherein no treatment was applied, second group in which the dissolved form of A. barbadensis in the soybean oil was applied (25 mg/day), and the third group which only soybean oil was applied (500 mg/day). Biopsy materials were taken from the lobus dexter of the livers of the rats and analyzed with light microscope after the necessary standard processing of histologic slides. Group I demonstrated normal structural characteristics of rat liver. In Group II and Group III, we observed nuclear enlargement, mild increase in chromatin and hydropic degeneration and binucleation in some hepatocytes. Liver histology demonstrated congestion in portal veins, sinusoids and the central veins. Merely in Group III, portal venous congestion and in Group II sinusoidal congestion was evident parenchyma of the liver. Additionally in Group III liver histology demonstrated plasmocyte infiltration in portal areas. Our study showed that using soybean with Aloe Barbadensis is synergystic and increasing each others effects. However we didn't observe mononeuclear infiltrations in Group II, these show antinflammatory effects of Aloe Barbadensis. It is determined that, depending on the used dose of Aloe Barbadensis, the toxic effect can change. If Aloe Barbadensis used very high doses it can have toxic effect on hepatocytes.

Peer Review #3 of "Sunset Yellow and Allura Red modulate Bcl2 and COX2 expression levels and confer oxidative stress-mediated renal and hepatic toxicity in male rats (v0.2)"

“…The measurement of antioxidant capacity is an appropriate indication for the total antioxidant defences of various kinds of tissues and organs (Ferrara, Gerber & LeCouter, 2003). Our study showed a reduced antioxidant capacity in rats treated with the dyes that can be attributed to the accelerated production of free radicals during (Kosif et al, 2010;Ramos et al, 2015). Meanwhile, the marked increase in infiltration of leucocytes implies a possible inflammatory response in affected tissues, as noted in earlier works (Soltan & Shehata, 2012;El-Desoky et al, 2017) following exposure to synthetic colouring agents in foods.…”

Sunset Yellow and Allura Red modulate Bcl2 and COX2 expression levels and confer oxidative stress-mediated renal and hepatic toxicity in male rats

“…Inflammation, necrosis and vacuolisation of the tubular epithelium occurred, in addition to the destruction of glomeruli, with thickening of Bowman’s capsule. The hydropic degeneration of hepatocytes and vacuolisation of tubular cells indicate the occurrence of intracellular oedema, as a result of toxicity or immune aggressions ( Kosif et al, 2010 ; Ramos et al, 2015 ). Meanwhile, the marked increase in infiltration of leucocytes implies a possible inflammatory response in affected tissues, as noted in earlier works ( Soltan & Shehata, 2012 ; El-Desoky et al, 2017 ) following exposure to synthetic colouring agents in foods.…”

Histopathological and biochemical assessment of d -limonene-induced liver injury in rats

“…The hydropic degeneration refers to the occurrence of reversible intracellular edema as a consequence of toxic, infectious or immune aggressions. The increase in cell volume and clearness is associated with the formation of microvacuoles or clear cytoplasmic spaces as a result of the action of injurious agents that cause intracellular accumulation of sodium and increase in water content [13] . These lesions were observed in the liver of all d -limonene-treated rats at both dose levels (25 and 75 mg/kg), but not in control and ethanol-treated animals.…”