Histopathological effects of larval trematodes on the digestive gland of freshwater snail species, Vivipara bengalensis and Lymnaea acuminata

Choubisa, S. L.; Sheikh, Zulfiya; Jaroli, V. J.

doi:10.1007/s12639-012-0116-1

Cited by 9 publications

(7 citation statements)

References 6 publications

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“…The hepatopancreas is an important digestive organ in P. canaliculata [ 16 ], and the expression of digestive enzyme genes in P. canaliculata may be closely related to nutrient metabolism and energy balance. The relative lack of information on enzyme genes has impeded research on the digestive physiology of P. canaliculata .…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of hepatopancreas transcriptomes in satiated and starving Pomacea canaliculata

2017

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BackgroundAlthough Pomacea canaliculata is native to South and Central America, it has become one of the most abundant invasive species worldwide and causes extensive damage to agriculture and horticulture. Conventional physical and chemical techniques have been used to eliminate P. canaliculata, but the effects are not ideal. Therefore, it is important to devise a new method based on a greater understanding of the biology of P. canaliculata. However, the molecular mechanisms underlying digestion and absorption in P. canaliculata are not well understood due to the lack of available genomic information for this species, particularly for digestive enzyme genes.ResultsIn the present study, hepatopancreas transcriptome sequencing produced over 223 million high-quality reads, and a global de novo assembly generated a total of 87,766 unique transcripts (unigenes), of which 19,942 (22.7%) had significant similarities to proteins in the UniProt database. In addition, 296,675 annotated sequences were associated with Gene Ontology (GO) terms. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was performed for the unique unigenes, and 262 pathways (p-value < 10−5) in P. canaliculata were found to be predominantly related to plant consumption and coarse fiber digestion and absorption. These transcripts were classified into four large categories: hydrolase, transferase, isomerase and cytochrome P450. The Reads Per Kilobase of transcript per Million mapped reads (RPKM) analysis showed that there were 523 down-regulated unigenes and 406 up-regulated unigenes in the starving apple snails compared with the satiated apple snails. Several important genes are associated with digestion and absorption in plants: endo-beta-1, 4-glucanase, xylanase, cellulase, cellulase EGX1, cellulase EGX3 and G-type lysozyme genes were identified. The qRT-PCR results confirmed that the expression patterns of these genes (except for the longipain gene) were consistent with the RNA-Seq results.ConclusionsOur results provide a more comprehensive understanding of the molecular genes associated with hepatopancreas functioning. Differentially expressed genes corresponding to critical metabolic pathways were detected in the transcriptome of starving apple snails compared with satiated apple snails. In addition to the cellulase gene, other genes were identified that may be important factors in plant matter metabolism in P. canaliculata, and this information has the potential to expedite the study of digestive physiology in apple snails.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0485-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparative profiling of hepatopancreas transcriptomes in satiated and starving Pomacea canaliculata

2017

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“…The study revealed severe damage to the cell constituents of the digestive and hermaphrodite glands in infected B. alexandrina and B. truncatus snails caused by trematode larvae. Changes in the digestive glands and ovotestis induced by larval digenean trematode parasites have been reported to depend on the severity of infection, larvae size, and types of larvae [ 98 ]. Possible explanations for these alterations include mechanical damage resulting from the migration, feeding, growth, and multiplication of trematode larvae, as well as physiological changes such as autolysis and/or necrosis.…”

Section: Discussionmentioning

confidence: 99%

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Dokmak,

Hammam,

Ibrahim

2024

Acta Parasit.

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Background Trematode infections of the genus Schistosoma can induce physiological and behavioral changes in intermediate snail hosts. This is because the parasite consumes essential resources necessary for the host's survival, prompting hosts to adapt their behavior to maintain some level of fitness before parasite-induced mortality occurs. Methods In this study, the reproductive and biochemical parameters of Biomphalaria alexandrina and Bulinus truncatus were examined during the cercareal shedding stage of infection with Schistosoma mansoni and Schistosoma haematobium, respectively, compared with controls. Results The study revealed an infection rate of 34.7% for S. mansoni and 30.4% for S. haematobium. In B. alexandrina infected with S. mansoni, a survival rate of 65.2% was recorded, along with a mean prepatent period of 30.3 ± 1.41 days, a mean shedding duration of 14.2 ± 0.16 days, and a mean lifespan of 44.1 ± 0.24 days. Meanwhile, in B. truncatus infected with S. haematobium, a survival rate of 56.4% was observed, with a mean prepatent period of 44.3 ± 1.41 days, a mean shedding duration of 22.6 ± 2.7 days, and a mean lifespan of 66.9 ± 1.6 days. Feeding increased in both infected species of snails, while the net reproductive rate (Ro) of the infected snails decreased. Total antioxidant (TAO) and lipid peroxidation activity increased in the two infected snail species during shedding, while Glutathione-S-transferase levels decreased. Lipid peroxidase activity and nitrogen oxide levels significantly decreased in infected B. alexandrina and increased in infected Bulinus. Steroid hormone levels were elevated in infected Biomphalaria, whereas they were reduced in infected Bulinus. Comet assay parameters showed an increase in the two infected genera after infection compared to control snails, indicating genotoxic damage and histopathological damage was observed. Conclusions These findings demonstrate that infection with larva species diverse biochemical, hormonal, genotoxic, and histopathological changes in the tissues responsible for fecundity and reproduction in B. alexandrina and B. truncates comparing with controls.

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“…The study found severe damage to the cell constituents of the digestive and hermaphrodite glands of infected B. alexandrina and B. truncatus snails caused by trematode larvae. Changes in digestive glands and ovotestis induced by larval digenean trematode parasites have been reported to be dependent on the severity of infection, size, and types of larvae (Choubisa et al, 2012). Mechanical damages resulting from the migration, feeding, growth, and multiplication of trematode larvae, as well as physiological changes such as autolysis and/or necrosis, are possible explanations for these alterations.…”

Section: Snail's Infection Ratementioning

confidence: 99%

Impact of Schistosoma sp., infection on biological, behavioral, physiological, histological, and genotoxicological aspects of Biomphalaria alexandrina and Bulinus truncatus snails

Dokmak,

hamam,

ibrahim

2023

Preprint

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Trematodes infection of genus Schistosoma can lead to physiological and behavioral changes in intermediate snail hosts. This is because the parasite consumes essential resources required for the host's survival, but the hosts can adaptively modify their behavior to ensure some level of fitness before parasite-induced mortality. The present study examined the reproductive and biochemical parameters of Biomphalaria alexandrina and Bulinus truncatus during the shedding stage of infection with Schistosoma mansoni and haematobium, respectively. The study found that the infection rate with S. mansoni was 34.7% and 30.4% with S. haematobium. In B.alexandrina infected with S. mansoni, a survival rate of 65.2% was recorded, along with a mean duration of shedding of 32.8 ± 5.5 days, a mean prepatent period of 37.5 ± 1.15 days, and a mean life span of 57.0 ± 1.21 days. Meanwhile, in B. truncatus infected with S. haematobium, a survival rate of 56.4% was recorded, with a mean duration of shedding of 42.6 ± 2.6 days, a mean prepatent period of 46.7 ± 2.3 days, and a mean life span of 65.9 ± 1.6 days. The feeding behavior was increased in the two infected species snail, while the net reproductive rate (Ro) of the infected species snails was reduced. Total antioxidant (TAO) and lipid peroxidation activity were increased in the two infected snails during shedding, while Glutathione-S-transferase was reduced. Lipid peroxidase (LPO) activity and nitrogen oxide (NO) levels decreased significantly in infected B. alexandrina and increased in infected Bulinus. Steroid hormone measurements were increased in the infected Biomphalaria, while they were reduced in infected Bulinus. Comet assay parameters were increased in the two infected genera after infection than control snails, and histopathological damage occurred. These observations demonstrated that infection initiates diverse biochemical, hormonal, genotoxic, and histopathological change to the tissues responsible for fecundity and reproduction in B. alexandrina and B. truncatus.

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Histopathological effects of larval trematodes on the digestive gland of freshwater snail species, Vivipara bengalensis and Lymnaea acuminata

Cited by 9 publications

References 6 publications

Comparative profiling of hepatopancreas transcriptomes in satiated and starving Pomacea canaliculata

Comparative profiling of hepatopancreas transcriptomes in satiated and starving Pomacea canaliculata

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Impact of Schistosoma sp., infection on biological, behavioral, physiological, histological, and genotoxicological aspects of Biomphalaria alexandrina and Bulinus truncatus snails

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