Histopathological evaluation of different methods of experimental induction of periapical periodontitis

Tanomaru, Juliane Maria Guerreiro; Leonardo, Mário Roberto; Silva, Léa Assed Bezerra; Poliseli-Neto, Ângelo; Tanomaru‐Filho, Mário

doi:10.1590/s0103-64402008000300012

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…After a 90-day experimental period, the animals were euthanized with an intravenous overdose of sodium pentobarbital. The maxillas and mandibles were dissected and sectioned to obtain individual roots that were fixed in sodium cacodylate solution with added sucrose and glutaraldehyde (17). The specimens were then washed and demineralized with an EDTA-based solution.…”

Section: Methodsmentioning

confidence: 99%

“…In case of disagreement, the specimen was re-evaluated and a consensus was reached between the examiners. Scores of A, B, C or D. from best to worst, were given to each of the following parameters (16,17): Intensity and extension of the inflammatory infiltrate, periodontal ligament thickness, bone and apical cementum resorption, and apical opening sealing ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

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Periapical repair after root canal filling with different root canal sealers

et al. 2009

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The aim of this study was to evaluate periapical repair after root canal filling with different endodontic sealers. Sixty-four root canals from dog s teeth were filled, divided into 4 groups (n=16). Root canals were instrumented with K-type files and irrigated with 1% sodium hypochlorite solution. Root canals were filled in the same session by active lateral condensation of the cones and sealers: Intrafill, AH Plus, Roeko Seal and Resilon/Epiphany System. After 90 days, the animals were euthanized and the tissues to be evaluated were processed and stained with hematoxylin and eosin. For histopathological analysis, the following parameters were evaluated: inflammatory process, mineralized tissue resorption, and apical mineralized tissue deposition. Histopathological analysis demonstrated that Intrafill had less favorable results in terms of apical and periapical repair, compared to the other sealers (p<0.05). AH Plus, Roeko Seal, and Epiphany sealers had similar and satisfactory results (p>0.05). In conclusion, AH Plus and the materials Roeko Seal and Epiphany are good options for clinical use in Endodontics.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Periapical repair after root canal filling with different root canal sealers

et al. 2009

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“…The second version of the in-vivo canine model was designed to evaluate the effects of classic endodontic filling materials on the healing of pre-existing apical periodontitis [94–98]. The experiments were similar to the first version, with the exception that the root canals were first exposed to the oral environment to induce the formation of periapical lesions over time [99] prior to shaping, cleaning and obturation of the canal spaces.…”

Section: The Classics (Traditional Gutta-percha and Sealer)mentioning

confidence: 99%

Ability of new obturation materials to improve the seal of the root canal system: A review

Li¹,

et al. 2014

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Objectives New obturation biomaterials have been introduced over the past decade to improve the seal of the root canal system. However, it is not clear whether they have really produced a three-dimensional impervious seal that is important for reducing diseases associated with root canal treatment. Methods A review of the literature was performed to identify models that have been employed for evaluating the seal of the root canal system. Results and Significance In-vitro and in-vivo models are not totally adept at quantifying the seal of root canals obturated with classic materials. Thus, one has to resort to clinical outcomes to examine whether there are real benefits associated with the use of recently-introduced materials for obturating root canals. However, there is no facile answer because endodontic treatment outcomes are influenced by a host of other predictors that are more likely to take precedence over the influence of obturation materials. From the perspective of clinical performance, classic root filling materials have stood the test of time. Because many of the recently-introduced materials are so new, there is not enough evidence yet to support their ability to improve clinical performance. This emphasizes the need to translate anecdotal information into clinically relevant research data on new biomaterials.

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“…The experimental induction of chronic periapical periodontitis in teeth in animals is important in the evaluation of new root canal materials and procedures for clinical use under similar conditions in human. 27 Periapical pathoses of pulpal origin develop in response to microbial irritants in the root canal system. Persistent injury to the dental pulp usually causes irreversible pulpitis and pulpal necrosis.…”

Section: Discussionmentioning

confidence: 99%

Morphological Effect of Diode Laser Irradiation of Periapical Lesion in Rat

Khan¹,

Kokubu²,

Tsuruoka³

et al. 2013

International Journal of Laser Dentistry

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The aim of this study was to evaluate the morphological effects of diode laser irradiation in the experimentally produced periapical lesion in rat. Fourteen adult male Sprague-Dawley rats weighing approximately 200 gm each were used. Pulp was extirpated from the mesial root of the maxillary first molar using 06 to 25 mm conventional reamers and files. After extirpation, the root canal was exposed to oral flora for 4 weeks to allow periapical periodontitis to develop. After the development of periapical periodontitis, the lesions were irradiated using a diode laser at 5 W for 5 seconds. The root canal was then sealed with cavity filling material for another 4-week period. After 4 weeks, the experimental rats were sacrificed by cervical dislocation. The maxillary first molar was then collected along with the surrounding tissue, which was processed in the laboratory. Hematoxylin and eosin and immunohistochemical staining were used to observe the morphological effects. Proliferating cell nuclear antigen (PCNA), STRO-1 and CD44 were used as the primary antibodies for the immunohistochemical study.A reduction in inflammatory cells, which were mainly composed of lymphocytes, was observed in the periapical lesions after irradiation. The number of PCNA-positive cells increased to approximately twice that observed in the nonirradiated control group. These PCNA-positive cells included STRO-1 and CD44-positive cells, indicating enhancement of wound healing and reduction in inflammatory cells.The present results showed that diode laser irradiation enhanced proliferation of PCNA-positive cells, which included STRO-1 and CD44-positive cells. This increase in these types of cell may improve wound healing in periapical lesions.

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Histopathological evaluation of different methods of experimental induction of periapical periodontitis

Cited by 9 publications

References 20 publications

Periapical repair after root canal filling with different root canal sealers

Periapical repair after root canal filling with different root canal sealers

Ability of new obturation materials to improve the seal of the root canal system: A review

Morphological Effect of Diode Laser Irradiation of Periapical Lesion in Rat

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