Histopathological evaluation of the effect of vitamin E on reversing changes induced by Tartrazine in parotid glands of albino rats

Abstract

Aim: Tartrazine (TZ) is an artificial azo dye made from coal tar. It is used all over the world as food coloring in a variety of foods, medicines, and cosmetics. was to assess the harmful effects of Tartrazine on the parotid gland and also study the reparative effect of Vitamin E on affected tissues. Materials and methods: Rats were grouped into Control group and experimental groups; tartrazine treated groups and Vitamin E and tartrazine treated groups. The control group was administered orally with water alone. Each subgroup consisted of 5 rats. All doses were given daily by oral intubation using a gastro pharyngeal tube. Sacrifice was carried out on two intervals: after14 days and after28 days The experimental group was administered orally with tartrazine (7.5 mg/kg, b.wt.). The specimens were collected after the study to be prepared for light microscopic examination. Results: The findings demonstrated that tartrazine caused severe histological changes in the parotid glands, including acini nuclei, changes in the cytoplasm, and loss of the normal architecture and ducts. Vitamin E treated group was nearly similar to control group and it obviously reversed the damage caused by Tartrazine. Conclusion: According to our study findings, it's interesting to note that vitamin E lowered the toxicity of Tartrazine and reversed the degeneration produced in the parotid gland tissues.