Histopathological features of infections caused by Fusarium oxysporum and F. solani in purple passionfruit plants (Passiflora edulis Sims)

Ortíz, E; Cruz, Marisol; Melgarejo, Luz Marina; Marquínez, Xavier; Hoyos-Carvajal, Lilliana

doi:10.1590/0100-5405/1910

Cited by 39 publications

(25 citation statements)

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“…Nevertheless, hypertrophy, cell wall degradation, losses of turgor pressure in the cells, and rot was observed in stem internal tissues. These symptoms are likely a sign of enzymatic activity produced by the fungus (Ortíz, et al, 2014;Köller, et al, 1982;Bateman & Basham, 1976 produce extracellular enzymes such as amylase, lipase, cellulase, protease, and lacase. Some similar symptoms of cell degradation have been reported in wheat infected by F. culmorum and F. graminearum (Jackowiak, et (Meiting & Shaosheng, 2010), potato infected by F. roseum (Mullen & Bateman, 1975), and maize infected by F. graminearum (Gao, et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Passionfruit is widely used in the food industry due to its edible qualities while passionflower (flowers of P. edulis) is recognized by its effects in the Central Nervous System and its implementation in traditional medicine in South America and India (Patel, 2009;Montanher, et al, 2007;Ocampo, 2007) (Ortíz, et al, 2014;Londoño, 2012;Gardner, 1989). Both F. oxysporum and F. solani produce structural damages in xylem, phloem, cambium, and parenchymatous cells (Saniewska, Dyki, & Jarecka, 2004;Bishop & Cooper, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…However, F. oxysporum penetrates through the roots and moves into the vascular system and F. solani f. sp. passiflorae concentrates in the collar area causing the collar rot disease of P. edulis (Ortíz, et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…This fact forces to eradicate complete crops and performs the cultivation in new areas free from the pathogen (Flores, et Histopathological features of plants of passionfruit infected by F. solani f. sp. passiflorae and its symptomatology associated with collar rot have been studied in detail (Ortíz, et al, 2014;Ortíz & Hoyos, 2012). However, damages produced by this pathogen in the surface of the plant and its progress over time in P. edulis f. flavicarpa are not well known.…”

Section: Introductionmentioning

confidence: 99%

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Environmental Scanning Electron Microscopy of the Infection Process of Fusarium solani f. sp. passiflorae in Seedlings of Passionfruit (Passiflora edulis f. flavicarpa)

Robledo-Buriticá

Ángel-García

Castaño-Zapata

2017

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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Environmental scanning electron microscopy (ESEM) was performed in seedlings of Passiflora edulis f. flavicarpa inoculated with Fusarium solani f. sp. passiflorae (teleomorph: Haematonectria haematococca) causal agent of passionfruit collar rot. Inoculations were carried out every 24 h until the seventh day and from this day until the fifteenth the interval of inoculation was 72 h. The pathogen isolated in PDA media was placed on the surface of the collar through the modified test-tube screening methodology. Epidermis of collar, stem, and leaves and longitudinal sections of collar and stem were observed. After 24 h of inoculation, conidia and dense septate mycelium were observed over the epidermis of the stem and the collar, respectively. Hypertrophy and cell wall degradation of the vascular tissues were also found during this period. Five days after the inoculation macroconidia were formed from monophialides in the aerial mycelium on the stem. Ten days after inoculation, xylem and pith cells of the collar were colonized by hyphae, inclusions, and mature sporodoquia over the stem surface. Hyphae colonization started six days after inoculation on stomata and thirteen days after inoculation monophialides with in situ microconidia were observed over the surface of the leaves. Based on the evidence and previous studies, internal hyphae colonization of F. solani f. sp. passiflorae concentrates on the collar area and the damages of the cells indicate an extracellular enzymatic activity of the fungus. The incubation and latent periods of F. solani f. sp. passiflorae were 1.4 and 4 days, respectively. © 2017. Acad. Colomb. Cienc. Ex. Fis. Nat.Key Words: Plant diseases; fungal diseases; Passifloraceae; collar rot; scanning microscopy. Microscopía electrónica de barrido ambiental del proceso de infección de ResumenSe realizó microscopía electrónica de barrido ambiental (ESEM) en plántulas de maracuyá (Passiflora edulis f. flavicarpa) inoculadas con Fusarium solani f. sp. passiflorae (teleomorfo: Haematonectria haematococca) agente causal de la secadera. Las inoculaciones se realizaron cada 24 h hasta el séptimo día y hasta el día quince el intervalo fue de 72 h. El aislamiento en medio de papa (PDA) se colocó sobre el cuello de la raíz mediante la metodología de cribado en tubos de ensayo modificada. La superficie del cuello, tallo, hojas y secciones longitudinales del cuello y tallo fueron observadas. Después de 24 h de la inoculación, se observaron conidios y micelio denso septado sobre la epidermis del tallo y el cuello e hipertrofia y degradación de la pared celular de los tejidos vasculares. Al quinto día se formaron macroconidios a partir de monofiálides en el micelio aéreo en el tallo. Diez días después, las células del xilema y médula del cuello fueron colonizadas por hifas, esporodoquios maduros e inclusiones. La colonización de las estomas por las hifas comenzó seis días después de la inoculación y trece días después de la inoculación se observaron monofiálides con microconidios in situ sobre la superfi...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental Scanning Electron Microscopy of the Infection Process of Fusarium solani f. sp. passiflorae in Seedlings of Passionfruit (Passiflora edulis f. flavicarpa)

Robledo-Buriticá

Ángel-García

Castaño-Zapata

2017

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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“…Once inside the root system, the fungi are initially limited to the root or the plant base and, at some point, begin to spread to the vascular system. The damage is due to colonization of xylem vessels by hyphae and microconidia; hypertrophy and hyperplasia of the cambium, xylem, and phloem; destruction of xylem fibers and amyloplasts in parenchymatous cells; and production of gels by the plant (Ortiz et al, 2014). Eventually, the combination of the fungal growth in the vascular system, fungal toxins such as naphthoquinones and fusaric acid (Rocha et al, 2016), and defense structures produced by the plant hamper the absorption and transport of water, causing wilt and death of the plant (Wheeler and Rush, 2001).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil

Dâ€TMAddazio¹,

Santos²,

Leitao³

et al. 2016

Afr. J. Microbiol. Res.

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The black pepper (Piper nigrum L.) is one of the most popular peppers in the world. Nonetheless, there are several limitations on cultivation, resulting in reduced production or a complete loss of the crop. The major disease affecting this crop is fusarium root rot caused by Fusarium solani f. sp. piperis, which is responsible for decimating whole crops in Brazil, with losses reaching millions of dollars per year. So far, there is no effective control measure against this fungus and no cultivars resistant to it. In this study, in vitro effects of different products on colony growth was evaluated. Carbendazim, chitosan, silicon, and phosphate were tested against F.solani f. sp. piperis isolates CML-2466, CML-2353, E-637, and E-596. Chitosan and silicon did not inhibit mycelial growth of any of the isolates, while phosphite inhibited mycelial growth by 100%. Carbendazim was found to be fungitoxic for isolates CML-2353 and E-596 and fungistatic for CML-2466 and E-637, inhibiting the mycelial growth of these isolates by 60 and 80%, respectively. There were no dose effects of the products tested.

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Effect of necrotrophic fungus and PGPR on the comparative histochemistry of Vigna radiata by using multiple microscopic techniques

Javed

Javaid

Hanif

et al. 2021

Microscopy Res & Technique

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Rapid advances in the field of pathogen detection have opened new opportunities and better understanding for their management approaches. Aim of this study was to elucidate histopathological observations of different tissues affected by Macrophomina phaseolina and to observe the defense responses of plant growth promoting rhizobacteria (PGPR) in mungbean plants. Sections of the stem and root were prepared and stained with ferric chloride, Lugol's iodine and Wiesner's reagent and were then observed under multiple microscopic techniques. Results revealed that both pathogen and PGPR produce responses on the plant that include colonization of xylem vessels by hyphae and sclerotia, hypertrophy and hyperplasia of the cells, destruction of xylem fibers and amyloplasts in parenchymatous cells; and production of gels by the plant were observed. There was a significant increase in lignin and phenolic compounds deposition in stem and root sections of PGPR treated and non‐treated mungbean plants. Whereas the soil amended with PGPR showed very less to no starch production. Moreover, production of gels and gums were also observed in both stem and root sections. Compared to light microscopy, scanning electron microscope provided greater depth of focus and resolution of the pathogen attack on plant tissues, associated bacteria. As a whole, the data demonstrated that inoculation of PGPR can be an effective strategy to stimulate plant growth and they could significantly activate disease resistance against M. phaseolina.

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Histopathological features of infections caused by Fusarium oxysporum and F. solani in purple passionfruit plants (Passiflora edulis Sims)

Cited by 39 publications

References 15 publications

Environmental Scanning Electron Microscopy of the Infection Process of Fusarium solani f. sp. passiflorae in Seedlings of Passionfruit (Passiflora edulis f. flavicarpa)

Environmental Scanning Electron Microscopy of the Infection Process of Fusarium solani f. sp. passiflorae in Seedlings of Passionfruit (Passiflora edulis f. flavicarpa)

Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil

Effect of necrotrophic fungus and PGPR on the comparative histochemistry of Vigna radiata by using multiple microscopic techniques

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