Histopathological features of liver damage induced by laser ablation in rabbits

Fujitomi, Yutaka; Kashima, Kenji; Ueda, Shinnya; Yamada, Yoshitake; Mori, Hiromu; Uchida, Yuzo

doi:10.1002/(sici)1096-9101(1999)24:1<14::aid-lsm4>3.3.co;2-u

Cited by 12 publications

(15 citation statements)

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“…This effect may be desirable in some cases, but may also present a limitation for HIFU ablation accuracy. Since cell death due to apoptosis occurs at lower thermal dose than thermal necrosis, the tissue adjacent to the HIFU target might be at risk from this effect (20).…”

Section: Tissue Heatingmentioning

confidence: 99%

HIFU for Palliative Treatment of Pancreatic Cancer

Khokhlova

Hwang

2016

Advances in Experimental Medicine and Biology

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KEY WORDSHigh intensity focused ultrasound (HIFU) is a novel non-invasive modality for ablation of various solid tumors including uterine fibroids, prostate cancer, hepatic, renal, breast and pancreatic tumors. HIFU therapy utilizes mechanical energy in the form of a powerful ultrasound wave that is focused inside the body to induce thermal and/or mechanical effects in tissue. Multiple preclinical and non-randomized clinical trials have been performed to evaluate the safety and efficacy of HIFU for palliative treatment of pancreatic tumors. Substantial tumor-related pain reduction was achieved in most cases after HIFU treatment, and no significant side-effects were observed. This review provides a description of different physical mechanisms underlying HIFU therapy, summarizes the clinical experience obtained to date in HIFU treatment of pancreatic tumors, and discusses the challenges, limitations and new approaches in this modality. therapeutic ultrasound; focused ultrasound; HIFU; pancreas cancer; review J Gastrointest Oncol 2011; 2: 175-184.

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Section: Tissue Heatingmentioning

confidence: 99%

HIFU for Palliative Treatment of Pancreatic Cancer

Khokhlova

Hwang

2016

Advances in Experimental Medicine and Biology

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“…This is supported by Goldberg et al (23), who reported the role of the adjuvant effect of chemotherapy on RF ablation in a subcutaneous tumor model and noted that at least 48 hours were required before coagulation fully evolved. The development and evolution of thermally ablated tissue over time at hematoxylin-eosin staining has been explored by others (24,25). It is agreed that a clear appearance of cell damage in the ablated area does not manifest on hematoxylin-eosinstained images within 1 or 2 days after thermal ablation.…”

Section: Experimental Studies: Contrast-enhanced Us For Monitoring Anmentioning

confidence: 99%

Real-time Monitoring of Radiofrequency Ablation and Postablation Assessment: Accuracy of Contrast-enhanced US in Experimental Rat Liver Model

Wu¹,

Wilkins²,

Ziats³

et al. 2014

Radiology

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Purpose:To examine the accuracy of the unenhanced zone at contrast material-enhanced ultrasonography (US) in predicting coagulative necrosis during and 21 days after radiofrequency (RF) ablation by using radiologic-pathologic comparison. Materials and methods:Animal studies were approved by the Institutional Animal Care and Use Committee. The livers of 28 rats underwent US-guided RF ablation. In four animals, contrast-enhanced US was performed during ablation and 2 hours and 2, 7, 14, and 21 days after ablation. The unenhanced zone area on US images was measured. DiI-labeled microbubbles were administered during ablation at 2, 4, and 6 minutes or at 2 hours and 2, 7, 14, and 21 days after ablation in the remaining 24 animals (n = 3 at each time point). One minute later, the animal was euthanized, and the ablated liver was harvested. Tissue samples were imaged to quantify total fluorescence, and NADH staining was performed on the same slice. Hematoxylin-eosin staining was also performed. The findings on fluorescence images, NADH-stained images, and hematoxylin-eosin-stained images were compared. The areas of DiI bubble-negative zones, NADH-negative zones, and lightly NADH-staining zones were measured. Data were analyzed by using one-way analysis of variance. Results:The area of the unenhanced zone on contrast-enhanced US images increased during RF ablation and reached a maximum within 2 days after ablation. At histopathologic examination, a transition zone manifested adjacent to the coagulation zone until 2 days after ablation. The DiI-bubble negative zone on fluorescence images and the damaged zone (transition zone plus coagulation zone) on NADH-stained images increased rapidly within 2 hours after ablation, then slowly reached the maximum on day 2. The ratios of the mean areas of these two zones at hour 2 to those at day 2 were 94.6% and 95.6%, respectively. High uniformity between the damaged zone on NADH-stained images and the DiI bubble-negative zone on fluorescence images was noted at all time points. Conclusion:The temporary transition zone in NADH staining is partially damaged and should transition to nonviability 2 days after ablation. These results demonstrate that contrast-enhanced US can help delineate the maximum area of cell damage (to within 5% of the maximum) as early as 2 hours after ablation. Contrast-enhanced US may be a simple and accurate tool for monitoring the effects of RF ablation and quantifying the size of thermal damage after treatment.q RSNA, 2013

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“…Previous studies reported that histopathological features of ablation zones induced by laser ablation or microwave ablation change over time. 14,15 They reported that the ablation zone was divided into two zones based on the histopathological features, central area with coagulation necrosis and peripheral area with dilated sinusoid and congestion of erythrocytes, and the size of the ablation zone increased progressively until several hours after ablation. Since RF ablation is also a thermal ablation method, its ablation zones might also change with time.…”

Section: Introductionmentioning

confidence: 99%

Chronological changes of radiofrequency ablation zone in rabbit liver: an in vivo correlation between gross pathology and histopathology

Song

Lee

Rhim

et al. 2017

The British Journal of Radiology

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Objective: To examine the gross pathology and histopathology of ablation zones created from radiofrequency (RF) ablation and to correlate their chronological changes. Methods: A total of 48 in vivo ablation zones (16 rabbit livers) were obtained immediately after and also 30 min, 1 h and 2 h after RF ablation and were subjected to haematoxylin and eosin (H&E) staining, nicotinamide adenine dinucleotide (NADH) diaphorase staining, terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining. Chronological changes in gross pathology and histopathology were evaluated and correlated with each other. Results: Peripheral red zones on gross pathology correlated with peripheral zones on H&E staining, lightly stained peripheral zones on NADH staining and peripheral positive zones on TUNEL staining. Central white zones on gross pathology correlated with combined central and border zones on H&E staining, central negative zones on NADH staining and combined central-positive and middle-negative zones on TUNEL staining. Boundary visibility between central white and peripheral red zones on gross pathology was significantly higher at 1 and 2 h than immediately after RF ablation. As time increased after RF ablation, visibility of the border zone on H&E staining and the grade of positively stained hepatocytes in the peripheral zone on TUNEL staining increased. Conclusion: Chronological changes in gross pathology of RF ablation zones correlated well with histopathology. The boundary between the central white and peripheral red zones tended to become clear at 1 h after RF ablation. Advances in knowledge: (1) RF ablation zones show chronological changes on gross pathology and histopathology. (2) Gross pathology and histopathology correlate well with each other.

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Histopathological features of liver damage induced by laser ablation in rabbits

Cited by 12 publications

References 0 publications

HIFU for Palliative Treatment of Pancreatic Cancer

HIFU for Palliative Treatment of Pancreatic Cancer

Real-time Monitoring of Radiofrequency Ablation and Postablation Assessment: Accuracy of Contrast-enhanced US in Experimental Rat Liver Model

Chronological changes of radiofrequency ablation zone in rabbit liver: an in vivo correlation between gross pathology and histopathology

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