Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis

Yahia, Samah Hassan; Etewa, Samia E.; Saleh, Nesreen S.; Mohammad, Samira; Aboulfotouh, Nora; Kandil, Ahmad; Sarhan, Mohamed

doi:10.1155/2022/2165205

Cited by 5 publications

(2 citation statements)

References 110 publications

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“…The disrupted liver architecture revealed by the present study in GII was previously reported as a result T. gondii infection [40,41].…”

Section: Discussionsupporting

confidence: 79%

Spiramycin-chitosan Nanoparticles Decline Parasite Burden and Renovate Patent Histopathological Changes in Liver and Lung in Mice Experimentally Infected with Acute Toxoplasmosis

Osama,

El-Dib,

khattab

et al. 2024

Egyptian Journal of Veterinary Sciences

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HE present study was designed to evaluate the use of nanoparticles in improving the antiparasitic effect of Spiramycin, Spiramycin loaded on Carboxymethyl chitosan nanoparticles (CMC Np), ) on experimental toxoplasmosis. Different doses of the drugs under study were assessed using parasitological and histopathological investigations. For this purpose, a total of 38 female Swiss albino mice were divided as follows; Group 1 (5 mice) as a negative control group (non-infected, non-treated group). Group 2 (5 mice) as a positive control group (infected, nontreated group); Group 3: In this group, 28 mice were infected, and treatment started after 10-15 days post-infection for 1 week on a daily basis. The third group was subdivided into four subgroups (7 mice for each), which were treated as follows; subgroup (3a); the infected mice treated with Spiramycin alone in a dose of 100 mg/ kg /day orally, sub group (3b); the infected mice treated with CMC Np orally. Sub group (3c); the infected mice treated with Spiramycin loaded on CMC Np of concentration of 0.35 gm/100 ml H 2 O (low dose-LD) orally. Subgroup (3d); the infected mice treated with Spiramycin loaded on CMC Np of concentration of 0.70 g/ 100 ml H 2 O (High dose-HD) orally. At the end of the experiment, liver and lung were dissected for detection of the parasite burden and the histopathological examination of the tissues was carried out to allocate the histopathological findings in these organs and detection of tissue cysts. Remarkably, a noticeable decrease in parasitic load was stated pooled with renovation of histopathological alterations were prominent with treated groups in association with infected non-treated group.

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“…The disrupted liver architecture revealed by the present study in GII was previously reported as a result T. gondii infection [40,41].…”

Section: Discussionsupporting

confidence: 79%

Spiramycin-chitosan Nanoparticles Decline Parasite Burden and Renovate Patent Histopathological Changes in Liver and Lung in Mice Experimentally Infected with Acute Toxoplasmosis

Osama,

El-Dib,

khattab

et al. 2024

Egyptian Journal of Veterinary Sciences

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“…The technique of immunohistochemistry (IHC) involves the utilization of an antigen-antibody reaction to visually assess the distribution and quantity of a certain molecule within different tissue samples. The procedure is conducted while preserving the histologic structure of tissues, so enabling the observation of the molecule's expression pattern within the microenvironment [16] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation the Sensitivity of three Immunological Diagnostic Technique for the diagnosis of Toxoplasmosis

Ahmed,

Al-Azizz,

jori

2024

PJBAS

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Background: Toxoplasmosis is widely recognized as a highly widespread parasitic infection in humans, capable of vertical transmission from mother to fetus. Clinical problems associated with toxoplasmosis during pregnancy include spontaneous miscarriage, premature labor, stillbirth, and fetal abnormalities. Objective: to evaluate the sensitivity of three immunological methods for the diagnosis of Toxoplasma. gondii in serum and placental tissue of aborted women. Materials and Methods:80 blood and placental Specimens were obtained from women who underwent abortion and were admitted to the Maternity ward and Child hospital in Kirkuk City. Blood samples were analyzed for anti-Toxoplasma antibodies IgM and IgG using ELISA and Combo Rapid test and placental samples were tested for T. gondii antigen in the placental tissue using the Immunohistochemistry technique. Results:The presence of anti-T. gondii antibodies in women who have undergone abortions was 30% (IgG) when the Combo Rapid test was used and 16.3% (IgG) when ELISA was used. In all cases, neither the combo rapid test nor the ELISA demonstrated seropositivity of IgM. IHC examination revealed that 60% of tissues had toxoplasmosis infection, whereas 40% of tissues were negative. Conclusion: the finding of this study indicates that IHC is a more sensitive immunological technique than a serological assay for the direct diagnosis of T.

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Spiramycin-loaded maltodextrin nanoparticles as a promising treatment of toxoplasmosis on murine model

Abdel-Wahab,

Shafey,

Selim

et al. 2024

Parasitol Res

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Despite being the initial choice for treating toxoplasmosis, sulfadiazine and pyrimethamine have limited effectiveness in eliminating the infection and were linked to a variety of adverse effects. Therefore, the search for new effective therapeutic strategies against toxoplasmosis is still required. The current work is the first research to assess the efficacy of spiramycin-loaded maltodextrin nanoparticles (SPM-loaded MNPs) as a novel alternative drug therapy against toxoplasmosis in a murine model. Fifty laboratory-bred Swiss albino mice were divided into five groups: normal control group (GI, n = 10), positive control group (GII, n = 10), orally treated with spiramycin (SPM) alone (GIII, n = 10), intranasal treated with SPM-loaded MNPs (GIV, n = 10), and orally treated with SPM-loaded MNPs (GV, n = 10). Cysts of Toxoplasma gondii ME-49 strain were used to infect the mice. Tested drugs were administered 2 months after the infection. Drug efficacy was assessed by counting brain cysts, histopathological examination, and measures of serum CD19 by flow cytometer. The orally treated group with SPM-loaded MNPs (GV) showed a marked reduction of brain cyst count (88.7%), histopathological improvement changes, and an increasing mean level of CD19 (80.2%) with significant differences. SPM-loaded MNPs showed potent therapeutic effects against chronic toxoplasmosis. Further research should be conducted to assess it in the treatment of human toxoplasmosis, especially during pregnancy. Graphical Abstract

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Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis

Cited by 5 publications

References 110 publications

Spiramycin-chitosan Nanoparticles Decline Parasite Burden and Renovate Patent Histopathological Changes in Liver and Lung in Mice Experimentally Infected with Acute Toxoplasmosis

Spiramycin-chitosan Nanoparticles Decline Parasite Burden and Renovate Patent Histopathological Changes in Liver and Lung in Mice Experimentally Infected with Acute Toxoplasmosis

Evaluation the Sensitivity of three Immunological Diagnostic Technique for the diagnosis of Toxoplasmosis

Spiramycin-loaded maltodextrin nanoparticles as a promising treatment of toxoplasmosis on murine model

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