Histopathological mapping of metastatic tumor cells in sentinel lymph nodes of oral and oropharyngeal squamous cell carcinomas

Denoth, Seraina; Broglie, Martina A.; Haerle, Stephan K.; Huber, Gerhard; Haile, Sarah R.; Soltermann, Alex; Jochum, Wolfram; Stoeckli, Sandro J.

doi:10.1002/hed.23782

Cited by 7 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible that these techniques may have revealed metastatic cells, previously unidentified microscopically. Step sections and immunohistochemistry are standard practice in assessment of human lymph nodes and have recently been evaluated in veterinary patients . It is likely that the use of immunohistochemistry would have increased the number of lymph nodes in our population identified as containing isolated tumour cells; however, no change would be expected in the number of lymph nodes with macrometastasis .…”

Section: Discussionmentioning

confidence: 99%

“…Step sections and immunohistochemistry are standard practice in assessment of human lymph nodes and have recently been evaluated in veterinary patients. 41,[55][56][57] It is likely that the use of immunohistochemistry would have increased the number of lymph nodes in our population identified as containing isolated tumour cells; however, no change would be expected in the number of lymph nodes with macrometastasis. 57 Ultimately, the use of step sections and immunohistochemistry would not be expected to alter the conclusions of this study and may have led to a further decrease in overall sensitivity of CT for lymph node metastasis.…”

Section: Limitationsmentioning

confidence: 94%

See 1 more Smart Citation

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Skinner

Boston

Giglio

et al. 2018

Vet Comparative Oncology

View full text Add to dashboard Cite

The diagnostic accuracy of contrast-enhanced CT for detection of cervical lymph node metastasis in dogs is unknown. The purpose of this retrospective, observational, diagnostic accuracy study was to assess the efficacy of CT for detection of mandibular and medial retropharyngeal lymph node metastasis in dogs. Histopathology of dogs with cancer of the head, CT and bilateral mandibular and medial retropharyngeal lymphadenectomy was reviewed. A single radiologist measured lymph nodes to derive short axis width and long-short axis ratios. Two blinded radiologists separately assessed lymph node margins, attenuation and contrast enhancement and each provided a final subjective interpretation of each node site as benign or neoplastic. Where radiologists' opinions differed, a consensus was reached. Sensitivity, specificity and accuracy were calculated for mandibular and medial retropharyngeal sites. Agreement between radiologists was assessed. Fisher's exact test and the Kruskal-Wallis H-test were used to assess associations between variables. Forty-one primary tumours were recorded in 40 dogs. Metastasis to mandibular or retropharyngeal lymph nodes occurred in 16 out of 40 dogs (43/160 nodes). Agreement between radiologists was almost perfect for margination, attenuation and enhancement, strong for interpretation of mandibular lymph node metastasis, and weak for interpretation of medial retropharyngeal lymph node metastasis. Sensitivity of CT was 12.5% and 10.5%, specificity was 91.1% and 96.7%, and accuracy was 67.5% and 76.3% for mandibular and medial retropharyngeal lymph nodes respectively. No individual CT findings were predictive of nodal metastasis. Given the low sensitivity of CT, this modality cannot be relied upon alone for assessment of cervical lymph node metastasis in dogs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Limitationsmentioning

confidence: 94%

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Skinner

Boston

Giglio

et al. 2018

Vet Comparative Oncology

View full text Add to dashboard Cite

show abstract

“…Seraina denoth et al 25 studied to assess the threedimensional distribution of metastatic tumour cells within sentinel node in oral squamous cell carcinoma. They concluded that with step serial sectioning the detection rate of micro metastasis and isolated tumour cells was increasing in subsequent sections, it was found that micro metastasis and isolated tumour cells were not randomly distributed, rather showed predominance in central planes close to the lymphatic inlet.…”

Section: Isolated Tumour Cellsmentioning

confidence: 99%

Sentinel Lymph Node- A Captain in the Management of Oral Squamous Cell Carcinoma

Bhopal¹,

Vadla²,

Mallikarjun³

et al. 2018

jemds

View full text Add to dashboard Cite

BACKGROUND Squamous cell carcinoma represents about 2-3% of all malignant neoplasms & 47% of those arising in Head & Neck area. Incidence of Oral Squamous Cell Carcinoma is increasing especially in young people. The status of lymph node involvement holds prime importance in the prognosis and therapy of Oral Squamous Cell Carcinoma (OSCC). The risk of neck metastasis depends on the site, size, grading and depth of infiltration of tumour. Neck dissection is the only surgical option for pathologic staging of neck in patients with OSCC. However, often a minimally invasive sentinel lymph node biopsy may be adequate. Sentinel Node biopsy is a novel useful technique alternative to neck dissection in the management of OSCC. Sentinel lymph node offers accurate staging with minimal morbidity. Selective excision, meticulous histopathological examination of sentinel lymph nodes play a very important role in preserving the surgical approach to a N0 carcinoma. Aim of this study was to evaluate the role of sentinel lymph node in staging and treatment of Oral Squamous Cell Carcinoma in a Tertiary cancer care center.

show abstract

“…58 According to Denoth et al, metastatic deposits are not randomly distributed within SLNs but are predominantly found in the central planes, closer to the lymphatic inlet; analysis of the distribution pattern of metastatic spread within SLNs with a virtual microscope resulted in a detection rate of 90% and 80% for micrometastasis and ITCs, respectively. 59 Van Den Berg et al introduced the concept of hybrid tracers containing both radioactive and fluorescent labels which allowed for the direct integration of preand intraoperative guidance technologies when used in combination with new surgical imaging modalities and navigation tools in SLN detection. 57 Using ultrasound-guided spectroscopic photoacoustic imaging of molecularly-activated plasmonic nanosensors in an OSCC murine model, Luke et al demonstrated that lymph node metastases as small as 50 μm could be detected in vivo at a depth of 1 cm with high sensitivity and specificity; this new approach could potentially be a sensitive alternative to a SLN biopsy.…”

mentioning

confidence: 99%

Sentinel Lymph Node Biopsy: A new approach in the management of head and neck cancers

Sharma¹,

Koshy²,

Grover³

et al. 2017

SQUMJ

View full text Add to dashboard Cite

‫شارما‬ ‫بوشان‬ ‫جروفر,‬ ‫سونال‬ ‫كوشي,‬ ‫جورج‬ ‫شارما,‬ ‫ديبيت‬ abstract: Cervical lymph node metastasis affects the prognosis and overall survival rate of and therapeutic planning for patients with head and neck squamous cell carcinomas (HNSCCs). However, advanced diagnostic modalities still lack accuracy in detecting occult neck metastasis. A sentinel lymph node biopsy is a minimally invasive auxiliary method for assessing the presence of occult metastatic disease in a patient with a clinically negative neck. This technique increases the specificity of neck dissection and thus reduces morbidity among oral cancer patients. The removal of sentinel nodes and dissection of the levels between the primary tumour and the sentinel node or the irradiation of target nodal basins is favoured as a selective treatment approach; this technique has the potential to become the new standard of care for patients with HNSCCs. This article presents an update on clinical applications and novel developments in this field. A s opposed to primary tumours, metastasis is responsible for the high mortality rate of most cancer patients; moreover, cancer cells primarily invade the regional lymph nodes before spreading to other parts of the body. Keywords1 Genetic instability results in tumour cell heterogeneity, leading to the emergence of metastatic clones and dissemination of the cancer from the primary tumour site.2 Malignant cells metastasise due to an interaction between the host factors and tumour cells. Genes related to the extracellular matrix, adhesion, motility and protease inhibition constitute a significant part of the metastatic process. The migration and invasion of cancer cells into the lymphatic system is governed by a variety of intricate genotypic, phenotypic and microenvironmental processes. After entering the lymphatic draining channels, the tumour cells metastasise to the regional lymph nodes in the neck and form the metastatic foci.4 Macrometastases refer to lymph nodes that appear suspicious on clinical or radiographical examinations; in contrast, nodal metastases-which are not detectable by imaging methods or physical examination-indicate occult or subclinical metastasis. Hermanek microscopically differentiated occult metastases into macrometastases (metastatic deposits of >2 mm), micrometastases (metastatic deposits of <2 mm) and isolated or small clusters of tumour cells (metastatic deposits of <0.2 mm).5 Isolated tumour cells (ITCs) can also be defined as a cluster of ≤200 tumour cells visible on one histology slide; these cells can further be categorised into those detectable by light microscopy, immunohistochemistry or molecular methods.6 Chemoradiation or elective neck dissection (END) should be considered in patients

show abstract

Histopathological mapping of metastatic tumor cells in sentinel lymph nodes of oral and oropharyngeal squamous cell carcinomas

Abstract: Based on the distribution we recommend an initial cut through the hilus and to proceed with the 4 most central 150-µm slices. Complete step sectioning is only required in case of a so far negative result.

Cited by 7 publications

References 41 publications

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Sentinel Lymph Node- A Captain in the Management of Oral Squamous Cell Carcinoma

Sentinel Lymph Node Biopsy: A new approach in the management of head and neck cancers

Contact Info

Product

Resources

About