Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in DBA/1J Mouse

IMAIZUMI, Kazunori; Hinoue, Hiromi; Ueno, Masashi; Takata, Isao; SATO, Tadashi; Minato, Yoshio; Takeshita, Masakazu; Okaniwa, Azusa

doi:10.1538/expanim1978.39.1_27

Cited by 7 publications

(7 citation statements)

References 9 publications

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“…A previous histological study of arthritic lesions induced by type II collagen in mice [8] revealed that the knee joints were most fre quently involved, whereas the present study in rats revealed that the tarsal joints were affected most frequently. Although the reason for such a discrepancy between rats and mice is obscure, it is of interest that the dominant site of the arthritic involvement differed between the two species.…”

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confidence: 72%

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Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats

Imaizumi¹,

Hinoue

Ueno

et al. 1994

Experimental Animals

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contrasting

confidence: 72%

“…Previ ously, we reported the histopathological characteristics of arthritic lesions and pathological evaluation of the effects of anti-rheumatoid drugs on collagen-induced arthritis in DBA/1J mice [8,9].…”

mentioning

confidence: 99%

Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats

Imaizumi¹,

Hinoue

Ueno

et al. 1994

Experimental Animals

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“…In particular, the tibia bone loss with the formation of pannus was very clear in this region [11]. Here, we show that shikonin modifies disease progression by abrogating soft tissue and bone lesions and arresting pannus development.…”

Section: Discussionmentioning

confidence: 53%

“…This model is useful for destruction of cartilage and bone, and is characterized by the increase of some cytokines in synovial fluids [10]. Decreased bone formation and increased bone resorption have been demonstrated during the development of polyarthritis; that is, serum osteocalcin levels and trabecular bone formation rate decreased during the first 2 weeks after injection of Freund's adjuvant [11].…”

Section: Introductionmentioning

confidence: 99%

The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis

Kim

Hong

Yim

2010

Korean J Physiol Pharmacol

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ABBREVIATIONS: RA, rheumatoid arthritis; BMD, bone mineral density; BMC, bone mineral content; CIA, collagen-induced arthritis; TIMP, tissue inhibitors of metalloproteinase; MMP, matrix metalloproteinase.Received April 9, 2010, Revised August 7, 2010, Accepted August 11, 2010 The potential therapeutic action of shikonin in an experimental model of rheumatoid arthritis (RA) was investigated. As a RA animal model, DBA/1J mice were immunized two times with type II collagen. After the second collagen immunization, mice were orally administered shikonin (2 mg/kg) once a day for 35 days, and the incidence, clinical score, bone mineral density (BMD), bone mineral content (BMC) and joint histopathology were evaluated. BMD in the proximal regions of the tibia largely increased in the shikonin treatment group compared with the control group. W e also examined the effect of shikonin on inflammatory cytokines and cartilage protection. Shikonin treatment significantly reduced the incidence and severity of collagen-induced arthritis (CIA), markedly abrogating joint swelling and cartilage destruction. Shikonin also significantly inhibited the production of matrix metalloproteinase (MMP)-1 and up-regulated tissue inhibitors of metalloproteinase (TIMP)-1 in mice with CIA. In conclusion, shikonin exerted therapeutic effects through regulation of MMP/TIMP; these results suggest that shikonin is an outstanding candidate as a cartilage protective medicine for RA.

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“…Macroscopical observation : Gross lesions of each joint in all animals were scored on the scale of 0-2 according to the scoring method reported previously (Arthritis score) [4], and the score of one animal was calculated as a total of scores of four limbs (the score will be 8 at the maximum). In the positive control group, clinical onset of arthritis was observed 4 or 5 weeks after the first immunization, and the arthritis score at 12 weeks after the immunization reached near the 8 point level which was the highest value.…”

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Pathological Evaluation of Anti-rheumatic Drugs on Type II Collagen-induced Arthritis in DBA/1J Mouse

Imaizumi¹,

Hinoue²,

Ueno³

et al. 1991

Experimental Animals

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The effects of anti-rheumatic drugs (lobenzarit (CCA) ; 10 and 50mg/kg, cyclophosphamide (CP) ; 5mg/kg and dexamethasone (DM) ; 0.25mg/kg) were evaluated immunologically and histopathologically on DBA/1J mice that develop polyarthritis after immunization by the intradermal injection of type II collagen. Serum anti-type II collagen IgG levels in the groups treated with CP and DM were significantly suppressed to 1/2 and 1/10 as compared to those of the positive control group, respectively. Those of both groups treated with CCA were not different from those of the positive control group. Histopathological examination revealed that treatment with CP and DM markedly reduced or suppressed inflammatory changes and resulted in low incidence of arthritis. From the standpoint mentioned above, treatment with anti-rhematic drugs suppressed the development of arthritis in this model, and we could confirmthat this model was useful for evaluation of the effect of anti-rheumatic drugs.DBA/ 1 J mouse is known to develop polyarthritis after immunization by the injection of type II collagen [2,3,6,14], and recently it arouses general interest as an experimental model of human rheumatoid arthritis. Previous histological examination revealed that the sequence of arthritic lesions was not uniform by each joint [41. Evaluation of the effects of anti-rheumatic drugs had been conducted in this model using macroscopic or immunologic criteria by Phadke et al. [10], Paska et al. [9] and etc.In the present study as the second series of experiments dealing with type II collagen induced polyarthritis in DBA/1J mouse, anti-rheumatic drugs of different categories were administered to the model animals and the limb joints were examined histopathologically to investigate the feasibility of this model for the evaluation of anti-rhematic agents.

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Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in DBA/1J Mouse

Cited by 7 publications

References 9 publications

Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats

Histopathological Study of Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats

The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis

Pathological Evaluation of Anti-rheumatic Drugs on Type II Collagen-induced Arthritis in DBA/1J Mouse

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