Histopathological Study of Protective Effects of Honey on Subacute Toxicity of Acrylamide-Induced Tissue Lesions in Rats’ Brain and Liver

Abstract

Background: The therapeutic potential of honey is related to antioxidant activity against reactive oxygen species because it contains compounds such as polyphenols; therefore, we evaluated the potential protective effect of honey on subacute toxicity of ACR by histopathologic study on tissue lesions in rat. Methods: In Ferdowsi University of Mashhad, Mashhad, Iran, 2016, male Wistar rats were divided into 7 groups. To induce toxicity, ACR was injected (50 mg/kg for 11 d) to rats in 5 groups. In treatment groups, rats received three doses of honey 1.25, 2.5, and 5 g/kg in addition to the ACR. The two remaining groups received vitamin E (200 IU/kg) and normal saline as positive and negative control respectively. On the last day, after necropsy, tissue specimens from brain and liver were collected for histopathological studies. Results: Receiving of ACR caused tissue injuries including degeneration, necrosis, hyperemia, hemorrhage and inflammation in liver; ischemic cell change, hyperemia, hemorrhage and edema in brain tissue. Administration of honey considerably reduced tissue damages caused by ACR, particularly with dosage 5 g/kg. Conclusion: The severity of tissue lesions caused by the ACR can be reduced by honey, likely through its antioxidant activity. Increasing concentrations of honey will enhance its effectiveness.

Effect of Honey and Aqueous Garlic Extracts against Short-Term Exposure of Cigarette Tobacco Smoking in Mice: Histopathological and Biochemical Investigations

“…When chrysin (a polyphenol available in honey) was administered intraperitoneally in various dosages, it prevented cigarette smoke-induced inflammation by decreasing the inflammatory cytokines and myeloperoxidase activity by lowering the phosphorylation of p38 and ERK [ 39 , 40 ]. Honey protects rat testes from smoking-induced histological alterations and oxidative stress, according to several studies [ 41 , 42 ]. Honey considerably decreased the harmful effects of cigarette smoking on testicular structures and oxidative stress by lowering lipid peroxidation and restoring the antioxidant system [ 43 , 44 ].…”

The Effects of Vitamin C on Histological Changes Induced by Acrylamide on Rat Ovary

“…The chemical structure of ACR and its metabolic conversion ability make it able to react with various cells. Various studies of the effects of ACR on various tissues such as the testis, liver, kidney, and brain have been investigated and it has been observed that It has led to changes in the tissues of the organs [6][7][8]. ACR affects especially the enzymatic and endocrine function of the testis [9] ACR causes cystic changes in the ovary and structural changes in the granulosa layer of the follicles.…”