Histopathological survey of the mussel Mytilus chilensis (Mytilidae) and the clam Gari solida (Psammobiidae) from southern Chile

Cremonte, Florencia; Puebla, Claudia; Tillería, Jorge; Videla, Viviana

doi:10.3856/vol43-issue1-fulltext-21

Cited by 18 publications

(8 citation statements)

References 10 publications

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“…The DN prevalence in this Gaydamak Bay population (4.0%) was probably underestimated, being based on flow cytometry, which is not very sensitive at the early stages of the disease 19,22 . Still, our estimate of DN level in the population from the Gaydamak Bay is close to the mean prevalence reported for Mytilus populations worldwide 39,[67][68][69][70] and much lower than the maximal prevalence of 56% reported for M. trossulus population from British Columbia 71 .…”

Section: Discussionsupporting

confidence: 75%

First description of a widespread Mytilus trossulus-derived bivalve transmissible cancer lineage in M. trossulus itself

Skazina

Odintsova

Maiorova

et al. 2021

Sci Rep

130

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Two lineages of bivalve transmissible neoplasia (BTN), BTN1 and BTN2, are known in blue mussels Mytilus. Both lineages derive from the Pacific mussel M. trossulus and are identified primarily by their unique genotypes of the nuclear gene EF1α. BTN1 is found in populations of M. trossulus from the Northeast Pacific, while BTN2 has been detected in populations of other Mytilus species worldwide but not in M. trossulus itself. Here we examined M. trossulus from the Sea of Japan (Northwest Pacific) for the presence of BTN. Using hemocytology and flow cytometry of the hemolymph, we confirmed the presence of disseminated neoplasia in our specimens. Cancerous mussels possessed the BTN2 EF1α genotype and two mitochondrial haplotypes with different recombinant control regions, similar to that of common BTN2 lineages. This is the first report of BTN2 in its original host species M. trossulus. A comparison of all available BTN and M. trossulus COI sequences suggests a common and recent origin of BTN2 diversity in populations of M. trossulus outside the Northeast Pacific, possibly in the Northwest Pacific.

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Section: Discussionsupporting

confidence: 75%

First description of a widespread Mytilus trossulus-derived bivalve transmissible cancer lineage in M. trossulus itself

Skazina

Odintsova

Maiorova

et al. 2021

Sci Rep

130

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“…Disseminated neoplasia (DN) has been previously observed in four of this species around the world: M. trossulus (Rasmussen 1986, Sunila 1987, Moore et al 1991, Elston et al 1992, Usheva and Frolova 2000, Vassilenko and Baldwin 2014, M. chilensis (Campalans et al 1998, Cremonte et al 2015, Lohrmann et al 2019, M. edulis (Farley 1969, Lowe and Moore 1978, Green and Alderman 1983, Elston et al 1988, Galimany and Sunila 2008 and M.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and polymorphism of a mussel transmissible cancer in Europe

Hammel

Simon

Arbiol

et al. 2021

Preprint

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Transmissible cancers are parasitic malignant cell lineages that acquired the ability to infect new hosts from the same species, or sometimes related species. First described in dogs and Tasmanian devils, transmissible cancers were later discovered in some marine bivalves affected by a leukemia-like disease. In Mytilus mussels, two lineages of Bivalve Transmissible Neoplasia (BTN), both emerged in a M. trossulus founder individual, have been described to date (MtrBTN1 and MtrBTN2). Here, we performed an extensive screening of genetic chimerism, a hallmark of transmissible cancer, by genotyping hundred SNPs of thousands of European Mytilus mussels. The genetic analysis allowed us to simultaneously obtain the genotype of hosts -M. edulis, M. galloprovincialis or hybrids- and the genotype of tumors of heavily infected individuals. In addition, a subset of individuals were systematically genotyped and analysed by histology in order to screen for possible non-transmissible cancers. We detected MtrBTN2 at low prevalence in M. edulis, and also in M. galloprovincialis and hybrids although at a much lower prevalence. No MtrBTN1 or new BTN were found but a few individuals with non-transmissible neoplasia were observed at a single polluted site on the same sampling date. We observed a diversity of MtrBTN2 genotypes that appeared more introgressed or more ancestral than MtrBTN1 and reference healthy M. trossulus individuals. The observed polymorphism is most likely due to somatic null alleles caused by structural variations or point mutations in primer-binding sites leading to enhanced detection of the host alleles. Despite low prevalence, two divergent sublineages, confirmed by mtCOI sequences, are co-spreading in the same geographic area, suggesting a complex diversification of MtrBTN2 since its emergence and host species shift.

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“…M. chilensis mussels are found along the western coast of South America, and disseminated neoplasia has been reported in multiple populations in Chile and Argentina since 1998 (Lohrmann et al, 2019; Cremonte et al, 2015; Campalans et al, 1998; Cremonte et al, 2011). Prevalence has varied by location, ranging from undetectable in some populations to as high as 12% in the Castro region of Chile and 13% in the Beagle Channel in southern Argentina, although no notable mass mortality events have been associated with neoplasia in these populations.…”

Section: Introductionmentioning

confidence: 99%

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

Yonemitsu

Giersch

Polo-Prieto

et al. 2019

eLife

Self Cite

264

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Transmissible cancers, in which cancer cells themselves act as an infectious agent, have been identified in Tasmanian devils, dogs, and four bivalves. We investigated a disseminated neoplasia affecting geographically distant populations of two species of mussels (Mytilus chilensis in South America and M. edulis in Europe). Sequencing alleles from four loci (two nuclear and two mitochondrial) provided evidence of transmissible cancer in both species. Phylogenetic analysis of cancer-associated alleles and analysis of diagnostic SNPs showed that cancers in both species likely arose in a third species of mussel (M. trossulus), but these cancer cells are independent from the previously identified transmissible cancer in M. trossulus from Canada. Unexpectedly, cancers from M. chilensis and M. edulis are nearly identical, showing that the same cancer lineage affects both. Thus, a single transmissible cancer lineage has crossed into two new host species and has been transferred across the Atlantic and Pacific Oceans and between the Northern and Southern hemispheres.

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Histopathological survey of the mussel Mytilus chilensis (Mytilidae) and the clam Gari solida (Psammobiidae) from southern Chile

Cited by 18 publications

References 10 publications

First description of a widespread Mytilus trossulus-derived bivalve transmissible cancer lineage in M. trossulus itself

First description of a widespread Mytilus trossulus-derived bivalve transmissible cancer lineage in M. trossulus itself

Prevalence and polymorphism of a mussel transmissible cancer in Europe

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

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