Histopathological surveys of the redclaw freshwater crayfish, Cherax quadricarinatus, in Australia

Edgerton, B. F.; Owens, Leigh

doi:10.1016/s0044-8486(99)00195-7

Cited by 37 publications

(55 citation statements)

References 23 publications

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“…Similarly, Edgerton et al (1995) found the presence of 2 species of rickettsiae, 1 in the cytoplasm of hepatopancreatic cells (Edgerton & Prior 1999) with mortalities up to 80% in several farms (Jimenez & Romero 1997). The bacteria exhibited morphology and characteristics similar to those reported by Edgerton et al (1995).…”

Section: Introductionmentioning

confidence: 92%

Infectivity, transmission and 16S rRNA sequencing of a rickettsia, Coxiella cheraxi sp. nov., from the freshwater crayfish Cherax quadricarinatus

Tan

Owens²

2000

Dis. Aquat. Org.

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Section: Introductionmentioning

confidence: 92%

Infectivity, transmission and 16S rRNA sequencing of a rickettsia, Coxiella cheraxi sp. nov., from the freshwater crayfish Cherax quadricarinatus

Tan

Owens²

2000

Dis. Aquat. Org.

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“…Spore size in histological sections can vary and it is therefore not appropriate to place taxonomic emphasis on such morphometrics (EDGERTON and ORWENS, 1999); for this reason we prefer define the detected pathogen organism as Thelohania-like.…”

Section: Thelohania-like Organismmentioning

confidence: 99%

Preliminary Investigations of Disease-Causing Organisms in the White-Clawed Crayfish Austropotamobius Pallipes Complex From Streams of Northern Italy

Quaglio

Morolli

Galuppi

et al. 2006

Bull. Fr. Pêche Piscic.

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A total of 99 live and 3 dead white-clawed crayfish Austropotamobius pallipes complex were collected during the summer and autumn of 2003 from one creek in Emilia Romagna region and four watercourses in Friuli Venezia Giulia region (Northern Italy) for experimental breeding and health status monitoring. Fifty-one animals from the Bidente River were maintained in tanks for breeding, and restocking, while another 30 specimens (27 living and 3 dead) were examined in the laboratory to evaluate their health status. Parasitological surveys were carried out on gills; mycological examinations on the exoskeleton (the walking legs and the abdomen); bacteriological examinations of inoculations from the haemolymph, and histopathological analysis on full transverse and sagittal sections of cephalothorax and abdomen.In the branchial-chambers, the gill filaments and the exoskeleton were observed for ectocommensal peritrich protozoans Epystilis spp. and Cothurnia sieboldii. In crayfish sampled from five streams and rivers, Branchiobdella italica, B. astaci, B. parasita and B. hexodonta were isolated. Heavily damaged gills in the presence of branchiobdellid cocoons were observed in the crayfish found dead in Orvenco creek.Hyaline and dematiaceous hyphomycetes were frequently observed and Fusarium spp. and Saprolegnia sp. were isolated from cuticular lesions.Mortalities occurred in two tanks, after 2 and 4 weeks respectively, in the adult crayfish coming from Bidente River utilized for breeding, with approximately a 23.5% cumulative mortality rate. In both cases the crayfish were weak, anorexic and lethargic. Bull. Fr. Pêche Piscic. (2006) 380-381 : 1271-1290 -1272 -Four and eight moribund samples respectively, coming from the two breeding tanks in which the mortalities occurred, underwent examination. In the first case the mortalities were attributed to Thelohania-like organisms and in the second case the mortalities were associated with enteric bacteria Citrobacter freundii.Key-words: white-clawed crayfish, Austropotamobius pallipes complex, diseases, Northern Italy. INVESTIGATIONS PRÉLIMINAIRES SUR LES PATHOLOGIES DES ÉCREVISSES À PATTES BLANCHES AUSTROPOTAMOBIUS PALLIPES COMPLEX DANS DES COURS D'EAU DU NORD DE L'ITALIE RÉSUMÉ

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“…Mostly, bacteria found in freshwater crayfish inhabit the ecosystem in which they live, may be found in water and sediments, and they reside on the exoskeleton, gills or in the gut. Bacteriological investigations of crayfish have predominantly been performed on their haemolymph using standard microbiological methods, and also by histopathological examinations of tissues (Colwell et al, 1975;Johnson, 1976;Scott and Thune, 1986;Madetoja and Jussila, 1996;Edgerton and Owens, 1999;Edgerton et al, 2002;Romero and Jiménez, 2002;Quaglio et al, 2006b;Jiravanichpaisal et al, 2009). When performing health status evaluations, considering correct identification and treatment of bacterial diseases and conditions, of (primarily) farmed crayfish, speed is always an issue.…”

Section: Introductionmentioning

confidence: 99%

High-throughput discrimination of bacteria isolated fromAstacus astacusandA. leptodactylus

Popović

Klobučar

Maguire

et al. 2014

Knowl. Managt. Aquatic Ecosyst.

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Key-words: crayfish, bacteria, MALDI-TOF MS, API 20EBacterial diseases and pathogens of crayfish are common, widespread, and occasionally causing serious mortalities. In order to take rapid measures for correct treatment of crayfish diseases, the turnover time and accuracy in bacterial identification is an issue. Bacteria isolated from tissues of apparently healthy Astacus astacus and A. leptodactylus were identified by the commercial phenotypic tests (API 20E) and by the matrix assisted laser induced desorption ionization connected to the time of flight mass spectrometry (MALDI-TOF MS). For Gram-negative rods, API 20E resulted in fewer species identifications than MALDI-TOF MS (5.2% versus 52.61%). The most frequently identified genus from A. astacus and A. leptodactylus was Pseudomonas spp.: API 20E (47.82%) and MALDI-TOF MS (52.17%). Both systems identified 60.86% of total isolates identically to the genus. Hafnia alvei was the only isolate for which API 20E and MALDI-TOF MS had a concordant reading to the species. MALDI-TOF MS proved to be a powerful, low-cost, rapid tool in bacterial genus identification. This is the first report of a direct comparison between the two systems for the identification of bacteria in crayfish, and also the first report on using MALDI-TOF MS for discrimination of freshwater crayfish bacterial isolates. ont identifié 60,86 % des isolats totaux au même genre. Hafnia alvei était le seul isolat dont API 20E et MALDI-TOF MS ont une lecture concordante à l'espèce. MALDI-TOF MS s'est avéré être un outil rapide, puissant, à faible coût, pour l'identification au niveau du genre bactérien. Ce travail est le premier d'une comparaison directe entre les deux systèmes pour l'identification des bactéries chez les écrevisses, et aussi le premier travail sur l'utilisation de MALDI-TOF MS pour la discrimination des isolats bactériens d'écrevisses d'eau douce.

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Histopathological surveys of the redclaw freshwater crayfish, Cherax quadricarinatus, in Australia

Cited by 37 publications

References 23 publications

Infectivity, transmission and 16S rRNA sequencing of a rickettsia, Coxiella cheraxi sp. nov., from the freshwater crayfish Cherax quadricarinatus

Infectivity, transmission and 16S rRNA sequencing of a rickettsia, Coxiella cheraxi sp. nov., from the freshwater crayfish Cherax quadricarinatus

Preliminary Investigations of Disease-Causing Organisms in the White-Clawed Crayfish Austropotamobius Pallipes Complex From Streams of Northern Italy

High-throughput discrimination of bacteria isolated fromAstacus astacusandA. leptodactylus

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