Histopathology and Histochemistry of Yersin Type Tuberculosis in Rabbits. Histochemical Study of Development of the Disease after Infection with Mycobacterium avium

Abstract

Cerny L.: Histopathology and Histochemistry of Yersin Type Tuberculosis in Rabbits. Histochemical Study of Development of the Disease after Infection with Mycobacterium avium. Acta vet. Brno, 48, 1979: 53-60. Organs of rabbits infected intravenously Mycobacterium avium were studied to reveal dynamics of changes in content of nucleic acids, carbohydrates and lipid substances and formation of fibres during development of Yersin type tuberculosis.Macrophages, young epitheloid cells and giant cells contained ribonucleic acid in their cytoplasm and nucleoli indicative of high activity of these cells. Gradually, amount of RNA in these cells diminished, first from their cytoplasm and then from the nucleoli and in prenecrotic stage the cells did not contain RNA at all.Along with the development of tuberculous process, in the cells of epitheloid and giant type content of PAS-positive carbohydrates increased. Greater part of these were glycogen while caseous material contained complexes of carbohydrates and proteins.In the cytoplasm of epitheloid and giant cells in the center of tuberculous foci, in necrotizing cells and in caseous material neutral fats, phospholipids, in later stages cholesterol and fatty acids were present. This finding showed that there was a metabolic disturbance of hypoxemic type.Formation of reticulin fibres in tuberculous lesions was connected with presence of acid mucopolysaccharides.