Histopathology and phylogenetic tree of Eimeria species in goat in Al-Diwaniyah Province

Abstract

The aim of this study which conducted in Al-Diwaniyah province during the period from October (2021) to April (2022) was to study histopathological examination of intestine and molecular identification with phylogenetic analysis of Eimeria. A total of 25 tissue samples (small intestine and large intestine) of goat were collected from slaughter houses in Al-Diwaniyah province. Histopathological study of the intestine revealed immature oocyst of the parasite in the colon, and the reproductive stages of the parasite were observed, represented by the presence of female (macrogametes) and male (microgametes), in addition to observing some histopathological changes represented by the presence of infiltration of lymphocyte and eosinophil of villi in goat. Genomic DNA was extracted from 100 goat´s fecal samples and18S rRNA gene of Eimeria was amplified by polymerase chain reaction. PCR technique showed that, out of 100 goat´s fecal samples 87(87%) were positive for 18S rRNA gene of Eimeria. Fifteen PCR positive product of local Eimeria isolates were sent to Bioneer company in Korea for detected the DNA sequence and compared it with that of other Eimeria published in GenBank.