Histopathology of experimental toxocariasis in mice

Zyngier, Felix R.

doi:10.1080/00034983.1974.11686939

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…Inflammation lasted for 2 3 months. Mixed cellular infiltrates in the airways of T. canis infected mice were also reported by Zyngier (1974) and Kayes & Oaks (1978). We found that, before escaping to immune privileged sites, the sojourn of larvae in the lungs was very brief (unpublished results).…”

Section: Discussionsupporting

confidence: 71%

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Toxocara canis‐induced murine pulmonary inflammation: analysis of cells and proteins in lung tissue and bronchoalveolar lavage fluid

Buijs

Lokhorst

Robinson

et al. 1994

Parasite Immunology

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The pulmonary immuno-inflammatory reaction and its effect on microvascular integrity was studied in Toxocara canis infected BALB/c mice. The investigation aimed to compare changes in lung histology and composition of bronchoalveolar lavage fluid (BALF) caused by T. canis infection with those described to occur in allergic asthma. Groups of (non)-infected mice (1000 ova) were investigated until 90 days post infection (p.i.). Migration of the larvae through the lungs was followed by a rapidly progressing multifocal interstitial and alveolar inflammation. Eosinophils and lymphocytes formed perivascular and partially peribronchial mixed cellular infiltrates. Lymphocytes with plasma cell morphology staining intracellularly for either alpha, epsilon or gamma immunoglobulins were demonstrated. BALF, collected from mice infected with either 250, 500 or 1000 ova was analysed at 14 and 28 days p.i. A dose-related increase in cell numbers and in albumin and IgA concentration was observed. IgE increase was independent of the infective dose. Peak values were measured at 14 days p.i. Albumin increase in lung homogenate was highest at 28 days p.i. 30% of the lymphocytes consisted of T cells carrying Thy-1,2 and L3T4 surface antigens. It is concluded that T. canis-induced pulmonary inflammation affects the permeability of the microvasculature. This is expressed by interstitial oedema and plasma exudation in the airway lumen. Both phenomena occur also in allergic asthma. It is suggested that increased permeability of the microvasculature is mediated by T cells and eosinophils.

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Section: Discussionsupporting

confidence: 71%

“…Larvae, hatched in the intestines, penetrate the epithelium and migrate to the somatic tissues. Released metabolic (Parsons, Bowman & Grieve 1986) and surface (Rockey et al 1983) antigens incite inflammation in various tissues (Zyngier 1974, Cookston, Stober & Kayes 1990). Airway disorders are described for man and animals (Taylor et at.…”

Section: Introductionmentioning

confidence: 99%

Toxocara canis‐induced murine pulmonary inflammation: analysis of cells and proteins in lung tissue and bronchoalveolar lavage fluid

Buijs

Lokhorst

Robinson

et al. 1994

Parasite Immunology

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“…The pattern of larval distribution after infection observed in the lungs and brain was similar to that described by other authors in gerbils (Cho et al , 2007; Alba-Hurtado et al , 2009) and in mice (Burren, 1968; Abo-Shehada & Herbert, 1984; Guardis et al , 2002; Pecinali et al, 2005). The observed pulmonary lesions, especially the haemorrhagic lesions, were similar to those described in mice (Mossalam et al , 1972; Zyngier, 1974). In the encephalon, larvae were found starting at 72 h post-infection, and were more abundant after 1 week, confirming previous reports in gerbils (Cho et al , 2007; Alba-Hurtado et al , 2009) and mice (Burren, 1968; Abo-Shehada & Herbert, 1984; Guardis et al, 2002).…”

Section: Discussionsupporting

confidence: 85%

“…The histological picture observed in the liver was similar to that observed in mice examined at the same time of infection (Zyngier, 1974). Focal inflammatory lesions and poorly organized granulomata were observed in the liver.…”

Section: Discussionsupporting

confidence: 79%

Larval migration of the ascarid nematode Toxocara canis following infection and re-infection in the gerbil Meriones unguiculatus

et al. 2015

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A morphological and immunohistochemical study of larval migration patterns was performed in gerbils that were infected once (primary infected group) or twice (secondary infected group) with 1500 eggs of Toxocara canis. Animals from the primary infected and the re-infected group were killed at different times after infection, and larvae were counted in the intestines, liver, lungs and brain. Fragments of all organs were formalin fixed and paraffin embedded for histology and immunohistochemistry analyses (using polyclonal anti-Toxocara serum raised in rabbits infected with T. canis). In the primary infected group, larvae were more abundant in the intestine at 24 h, in the liver and lungs between 24 and 72 h and in the brain after 96 h; larvae predominated in the brain for up to 60 days after infection. In the re-infected group, an increase in the number of larvae in the liver and a reduction in the number of larvae in the brain was observed up to 60 days after re-infection. Inflammatory reactions were absent or limited. Eosinophils and loose granulomata were observed around the larvae and their antigens in the primary infected group and were more severe. Many eosinophils and typical epithelioid granulomata were observed around larvae in the re-infected group. These results demonstrate that the migration pattern of T. canis larvae in gerbils is similar to that in mice and rats, exhibiting a late neurotropic stage. In the re-infected group, there was histological evidence of an adaptive T-helper 2 (Th-2) response, and larvae were apparently retained within granulomata in the liver, without obvious signs of destruction.

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“…De igual forma coincidimos con quienes señalaron que las alteraciones por este ascaridio no solo afectan la retina sino otras estructuras del polo posterior (1)(2)(3)(4)(5)(6)(7)(8)(9). Por otra parte, también se afecta el polo anterior como las observadas en cornea, cristalino y cámara anterior, donde notamos alteraciones histológicas de su citoarquitectura normal, acompañadas de edema intenso y reacción inflamatoria granulomatosa.…”

Section: Discussionunclassified

Infección experimental en ratones con larva migrans ocular

Stangogiannis

Marval²,

De³

et al. 2007

Arch Soc Esp Oftalmol

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RESUMENObjetivo: Caracterizar las posibles alteraciones histológicas en el globo ocular de ratones infectados masivamente con Toxocara canis, durante los estadios agudos y crónicos de la infección. Método: Se realizó un estudio experimental, prospectivo in vivo, con globos oculares derechos e izquierdos correspondientes a cinco grupos de cuatro ratones cada uno (dos blancos (A/J) y dos negros (C57/BL) infectados por vía endovenosa con 1.000 huevos embrionados de Toxocara canis. Los ratones fueron sacrificados de forma secuencial post infección (3-180 días), se realizó microscópica convencional e histopatología fotografiadas. Resultados: Todos los ratones mostraron algún grado de daño ocular en uno o ambos ojos. La retina era la capa más afectada con edema, hemorragia y cambios degenerativos severos. Otras alteraciones incluyeron reacción inflamatoria de la córnea, obliteración de la cámara anterior con material fibrinoide, asimismo, se evidenció cambios en la histología normal de la uvea y del cristalino. En dos ojos de ratones se encontraron segmentos de larva en la retina, sin reacción inflamatoria alrededor de ellos. ARTÍCULO ORIGINAL ABSTRACTObjective: To characterize the histologic alterations in the ocular globe of mice infected with eggs of Toxocara canis, during both the acute and chronic stages of the infection. Method: Performance of an experimental, prospective study in vivo, with right and left ocular globes [using 5 groups of 4 mice in each study; 2 white (A/J) and 2 black (C57/BL)], infected by the intravenous injection of 1000 embrionated Toxocara canis eggs. The mice were sacrificed sequentially post infection (3-180 days), the eyes evaluated by conventional microscopic techniques and the histopathology findings recorded. Results: All the mice revealed some degree of ocular damage in one or both eyes. The retina was the most affected layer with edema, hemorrhage and severe degenerative changes seen. Other alterations included inflammation of the cornea, and obliteration of the anterior chamber with fibrinoid material. We also demonstrated changes in the normal histology of the uvea and lens. In only two eyes were segments of larvae found in the retina, and in each instance there was no evidence of an inflammatory reaction around them.

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Histopathology of experimental toxocariasis in mice

Cited by 8 publications

References 14 publications

Toxocara canis‐induced murine pulmonary inflammation: analysis of cells and proteins in lung tissue and bronchoalveolar lavage fluid

Toxocara canis‐induced murine pulmonary inflammation: analysis of cells and proteins in lung tissue and bronchoalveolar lavage fluid

Larval migration of the ascarid nematode Toxocara canis following infection and re-infection in the gerbil Meriones unguiculatus

Infección experimental en ratones con larva migrans ocular

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