Histopathology of Heart of Freshwater Spiny Eel, Mastacembelus armatus Naturally Infected with Tetracotyle Metacercaria (Trematoda: Strigeidae)

Vankara, Anu Prasanna; Chikkam, Vijayalakshmi

doi:10.3923/jp.2013.45.54

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…They use these compounds to invade the host tissue and get nutrients for themselves ( 24 ). Infection with metacercariae of various digenean is often accompanied by increased eosinophils ( 25 , 26 ). Adeyemo and Agbede ( 25 ) observed eosinophilic granular cell growth in the gills of Oreochromis niloticus parasitized with C. tilapia metacercariae.…”

Section: Discussionmentioning

confidence: 99%

“…Adeyemo and Agbede ( 25 ) observed eosinophilic granular cell growth in the gills of Oreochromis niloticus parasitized with C. tilapia metacercariae. In the heart of Mastacembelus armatus infected with Tetracotyle metacercariae, an increase in the number of eosinophilic granular cells was reported in the region between the host tissue and the parasite ( 26 ). In addition, histopathological alterations of Nandus nandus parasitized with C. complanatum were seen in the liver, characterized by loosening of hepatic tissue, eccentrically located nuclei of hepatocytes, and necrosis ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

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Pathological effects due to metacercariae of Clinostomum piscidium migration in snakeskin gourami (Trichopodus pectoralis) in Thailand

2023

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Clinostomum spp. is a fish-borne pathogen and a digenetic trematode with a global range. Despite its zoonotic relevance, the pathogenic impact of the parasite in Thai aquaculture is currently unclear. The present study deals with the pathogenic changes that fluke causes in their host, Trichopodus pectoralis, and the molecular confirmation of the Clinostomum piscidium by targeting 18 s rDNA and ITS gene. The metacercariae of C. piscidium were discovered in the body cavity of infected fish. The gross pathological examination revealed a few white migratory tracks on the surface of the liver and spleen. The migratory track showed histologically as a primary hemorrhage and necrosis of hepatic cells surrounded by a layer of macrophages and epithelioid cells, inflammatory cells, and eosinophilic granular cells in the cytoplasm of liver cells and close to the epithelial cells of the intestine. Also, the migratory track in the spleen appeared as a marked decrease of Red Blood Cell (RBC) count and changes in the necrotic tissue. Infection with this metacercaria produced hepatic tissue injury, which disrupted hepatic metabolism and decreased body weight in the fish hosts. The findings of the study suggest that the pathological effect of C. piscidium on farm T. pectoralis can cause significant economic loss by stunting fish development and predisposing fish to opportunistic pathogens in the environment. Hence, the treatment and control of C. piscidium infections are crucial for the viability of the aquaculture sector since this parasite has been found to cause pathological damage to the vital organs of fish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pathological effects due to metacercariae of Clinostomum piscidium migration in snakeskin gourami (Trichopodus pectoralis) in Thailand

2023

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“…These compounds are utilized to infiltrate the host tissue and acquire nutrients for their own sustenance (Kasny et al, 2009). Increased eosinophils often accompany infection with metacercariae of different digenea (Adeyemo and Agbede, 2010;Prasanna and Chikkam, 2013). Oreochromis niloticus, which was parasitized by C. tilapia metacercariae, had an increase in eosinophilic granular cells in the gills, according to Adeyemo & Agbede (2008).…”

Section: Discussionmentioning

confidence: 99%

“…Oreochromis niloticus, which was parasitized by C. tilapia metacercariae, had an increase in eosinophilic granular cells in the gills, according to Adeyemo & Agbede (2008). Tetracotyle metacercariae infection of Mastacembelus armatus resulted in an increase in eosinophilic granular cells in the area between the host tissue and the parasite (Prasanna and Chikkam, 2013). According to Milbourne and Howell (1993), the ES product from the fluke has similar biological properties to interleukin-5 (IL-5), a cytokine that stimulates myeloid precursor cells to differentiate and activate eosinophils.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Clinostomum spp. infecting cultured Snakeskin gourami (Trichopodus pectoralis) in Thailand

Islam

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The digeanean Clinostomum spp. is a significant parasitic pathogen of freshwater fish. It has low host specificity and a wide distribution geographically. Morphological and molecular data analyses have been performed on Clinostomum to assess its diversity. However, the molecular occurrence of Clinostomum spp. in aquaculture in Thailand has yet to be reported. Snakeskin gourami (Trichopodus pectoralis), a freshwater fish cultured in Thailand, has significant economic importance. Herein, the study focuses on describing a digenean Clinostomum in the abdominal cavity of T. pectoralis and reports the prevalence of this parasite from central Thailand. Two hundred and sixty-two 4 to 8- month-old T. pectoralis were obtained from Samut Sakorn, Samut Songkhram, Samut Prakarn, and Kanchanaburi provinces of central Thailand. We measured their overall length as well as their body weight. The body cavity of T. pectoralis was examined, and metacercariae of C. piscidium were discovered inside. In the abdominal cavity of infected fish, the parasites were discovered either free or adhered to adipose tissue and the outer membrane of the visceral organs. It was found that the total prevalence was 13.35 %, and the parasite intensities were found to be higher in the females taken from the farms than in the males (P<0.05). Pathological examination showed a few white migratory patterns on liver and spleen cells. The track presented histologically as the main central hepatic necrosis and hemorrhage surrounded�by layers�of macrophages and epithelioid cells. This metacercaria infection caused damage to the fish hosts' hepatic tissue, which disrupted their hepatic metabolism. As a result, the fish hosts experienced a slowdown in their rate of development and a reduction in their total body mass. In addition, morphological and molecular characterization of species was conducted using 18S rDNA and inter-transcribed spacer (ITS1 and ITS2) sequence data. A BLAST search of 18S rDNA sequence revealed 100% sequence similarity with C. piscidium (FJ970655) infecting banded gourami (Colisa fasciata) from India and 9098% sequence similarity with other clinostomid in Australia, USA, China, Israel, and Italy. ITS sequence data also revealed 100% sequence homology with the C. pisicidium isolated from C. fasciata from India. Based on phylogenetic analysis, this study reports the first molecular identification of C. piscidium species in cultured T. pectoralis in Thailand. �

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Description of Tetracotyle wayanadensis n. sp. (Digenea: Strigeidae) metacercaria infecting six species of freshwater fishes from Western Ghats, India

Jithila

Prasadan

2018

J Parasit Dis

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n. sp. (Digenea, Strigeidae) infecting different organs of six species of freshwater fishes, (eye, buccal cavity), (eye, buccal cavity), (operculum, heart, mesenteries), (brain), (muscles, mesenteries) and (mesenteries) collected from water bodies in the Wayanad region of the Western Ghats is described and illustrated. n. sp. is new to the genus and is separated from its congeners on the basis of differences in morphology and morphometry. The present paper also describes the prevalence, intensity, mean abundance and sites of infection of the parasite in each host. The prevalence of infection of n. sp. in is 7.5%, intensity of infection is 2.66 and mean abundance is 0.2; in 10%, 6 and 0.6; in 12.2%, 6.2 and 0.76; in 7.14%, 5 and 0.36; in 100%, 2 and 2; in 10%, 2 and 0.2 respectively.

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Histopathology of Heart of Freshwater Spiny Eel, Mastacembelus armatus Naturally Infected with Tetracotyle Metacercaria (Trematoda: Strigeidae)

Cited by 6 publications

References 12 publications

Pathological effects due to metacercariae of Clinostomum piscidium migration in snakeskin gourami (Trichopodus pectoralis) in Thailand

Pathological effects due to metacercariae of Clinostomum piscidium migration in snakeskin gourami (Trichopodus pectoralis) in Thailand

Molecular characterization of Clinostomum spp. infecting cultured Snakeskin gourami (Trichopodus pectoralis) in Thailand

Description of Tetracotyle wayanadensis n. sp. (Digenea: Strigeidae) metacercaria infecting six species of freshwater fishes from Western Ghats, India

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