Histopathology of Liver and Cardiac Muscle of Alloxan-Induced Diabetic Rats (Rattus norvegicus) After Administration of Bosibosi (Timonius flavescens (Jacq.) Baker) Leaves Ethanol Extract

Abstract

This research was aimed to study the effect of ethanol extract of bosibosi leaves (Timonius flavescens) (EEBL) on histopathology of liver and cardiac muscles of diabetic male rats. Animals (25) were randomly distributed into groups of NC (normal + NaCl), DC (diabetes + NaCl), DM (diabetes + metformin), DE300 (diabetes + EEBL 300) and DE500 (diabetes + EEBL 500 mg/kg bw/day); treated for 28 consecutive days and sacrificed for tissue collection. Tissues were processed (formalin, paraffin, H & E) and examined for histopathology. All diabetic rats showed hepatic tissue necrosis which is characterized by pycnotic nuclei, karyorhexis and karyolysis. The order of groups based on the percentage tissue damage is NC ( 27.61±2.44), DM (38.62±3.87), DE500 (45.15±3.76), DE300 (61.20±5.51) and DC (75.17±3.21). Cardiac muscle shows cell damage form mild degeneration to necrosis. The results indicate the potential of EEBL to repair tissues damaged by diabetes.