Historadiography

Engström, Arne

doi:10.1016/b978-1-4831-6744-2.50018-7

Cited by 4 publications

(1 citation statement)

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“…This value, lying close to the centre of the range reported for various cell types, was used for calculating dry mass. Similarly, as extraction procedures indicated that about 70 % of the dry mass of hypoglossal nerve cell bodies is protein, a finding conforming with analyses of Deiter neurones (BrattgArd & Hyden, 1952) and hypoglossal neurones (Brattgard, Edstrbm & Hyd6n, 1957) of the rabbit by X-ray absorption historadiography (Engstrom, 1956), a value of 0-0018 was used as the specific refraction increment. The use of these values was justified by comparing the values obtained for ribonuclease extractable RNA of nerve cell bodies and of nucleoli using interference microscopy with values obtained using ultra-violet absorption microspectrography.…”

Section: Methodsmentioning

confidence: 99%

The response of motor neurones to intramuscular injection of botulinum toxin

Watson¹

1969

The Journal of Physiology

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SUMMARY1. The dry mass and nucleic acid content of both nerve cell bodies and their nucleoli were measured by interference microscopy and ultra-violet absorption microspectrography respectively: succinoxidase and acetylcholine hydrolase activities were also determined. Autoradiography was used to follow synthesis of deoxyribonucleic acid (DNA) by glial cells, and to follow nucleic acid and protein metabolism in muscle fibres.2. After injection of botulinum toxin the synthesis of ribosomal RNA by the neurone followed closely the pattern found after axotomy.3. After injection of toxin neuronal dry mass increased before the rate of ribosomal RNA synthesis was raised. This early increase, which was not due to increased protein synthesis, probably represents a 'damming back' of proteins within the nerve cell body.4. After injection of toxin no local accumulation of microglial cells synthesizing DNA was found around the affected neurones: it is suggested that this reflects the intact system for intra-axonal transport under these conditions. 5. The affected muscles show increased nucleic acid and protein synthesis.6. It is suggested that the results obtained indicate that membrane expansion or synthesis which occurs both in muscle and in neurone under these circumstances is the factor responsible for inducing directly or indirectly the changes found in nucleic acid metabolism after injection of botulinum toxin and after axotomy.

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Section: Methodsmentioning

confidence: 99%