Nearly every study dealing with temperature extremes underscores the lack of a universal and broadly used method of identifying such events. The most popular are relative methods, which are based on the empirical distribution of temperature at each location (i.e., percentiles). The aim of this study was to evaluate the effects of the various percentile-based methods of defining hot days on the analysis of their frequency of occurrence, trends, and geographic patterns in summer in Europe. The basis for the research consists of daily maximum (TX) and minimum (TN) values of air temperature for 1961-2017 for Europe obtained from the E-OBS database. A hot day occurs when air temperature exceeds the 90th percentile-based threshold. These thresholds are determined using the following: (I) various temperature metrics (TX and TN), (II) various baseline periods (1961-1990, 1971-2000, 1981-2010), and (III) different timeframes within the year that the percentile is calculated for (summer season, separate summer months, and each calendar day). Our results indicate that the use of different variants of the percentile-based definition leads to differences in the geographic patterns of frequencies of and trends in summer hot days in Europe. The differences are especially substantial within the results obtained using various temperature metrics and baseline periods, and they are relatively small when different timeframes within the year that the percentile is calculated for are considered. On the example of the case study, we also show how the use of different research approaches may affect the intensity and spatial extent of an extreme temperature event.