History of Gumboro (infectious bursal disease) in Pakistan

Khan, Rashid Ahmed; Sajid, Sanaullah; Habib, Md. Ahasan; Ali, Waqas; Shah, Muhammad Arif; Sarfraz, Maliha

doi:10.1016/j.jsps.2017.04.005

Cited by 21 publications

(13 citation statements)

References 22 publications

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“…In the late 1980s, vvIBDV (also known as hyper virulent strain (hvIBDV)) was first reported in Europe (Heine et al 1991), which is responsible for causing severe (up to 100%) mortality in immature chickens (Chettle et al 1989). Importantly, strains of vvIBDV have rapidly circulated worldwide, being enzootic in Europe, America, Asia, and Africa (Jackwood and Nielsen 1997;Zorman et al 2003;Banda et al 2003;Stoute et al 2013;Mohamed et al 2014;Khan et al 2017). IBD mainly affects the bursa of Fabricius with substantial destruction of B lymphocytes (Käufer and Weiss 1980).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

Zafar

Shah

Shehzad

et al. 2020

Appl Microbiol Biotechnol

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Birnaviridae is a family of viruses (birnaviruses) which consists of four genera, members of which cause diseases in fish, birds, mollusks, and insects. The genome of birnaviruses encodes the highly immunogenic VP2 capsid protein. In order to demonstrate that the VP2 protein can be exploited as a diagnostic antigen for birnaviruses, we developed a lateral flow assay based on the surface-exposed VP2 protrusion domain of a representative birnavirus, infectious bursal disease virus (IBDV) of serotype 1 which causes the highly devastating infectious bursal disease in chickens. The biophysical characterization of the purified domain reveals that the domain predominantly consists of β-sheets, exists in a trimeric form, and remains folded at high temperatures, making it suitable for diagnostic purposes. Owing to its highly immunogenic nature and excellent biophysical properties, we employed the VP2 protrusion domain in a gold nanoparticle-based lateral flow assay for rapid detection of anti-IBDV antibodies in serum samples of infected chickens. Our results indicate that the domain binds anti-IBDV antibodies with high specificity during laboratory testing and on-site testing. The lateral flow assay reported here yields comparable results in a qualitative manner as obtained through a commercial enzyme-linked immunosorbent assay (ELISA). As VP2 is a common capsid protein of birnaviruses, the lateral flow assay can be generalized for other birnaviruses, and members of Tetraviridae and Nodaviridae families which contain homologous VP2 capsid proteins. Keywords Birnavirus . Circular dichroism . Gold nanoparticle-based lateral flow assay . Infectious bursal disease virus . Infrared spectroscopy . VP2 protrusion domain Electronic supplementary material The online version of this article (https://doi.

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Section: Introductionmentioning

confidence: 99%

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

Zafar

Shah

Shehzad

et al. 2020

Appl Microbiol Biotechnol

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“…During postmortem diagnosis, the chickens showed hemorrhages in the pectoral or thigh muscles, dehydration, urate deposits within the kidneys, enlarged, edematous, hyperemic and or atrophic bursa of Fabricius (Aliyu et al, 2016;Khan et al, 2009). In chronic cases, presence of hemorrhage within the connection between gizzard and proventriculus is observed (Khan et al, 2017;Aliyu et al, 2016). The virus generally infected the lymphoid tissue especially the bursa (Dey et al, 2019;Wang et al, 2011;Van den Berg et al, 2000) and thus others immune organs like as spleen, bone marrow, thymus are also involved.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

Afrin¹,

Chowdhury²,

Cho³

et al. 2020

Afr. J. Microbiol. Res.

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Infectious Bursal Disease (IBD) is an economically important irreversible immunosuppressive disease of young birds. The present study was designed to confirm the efficacy of two common diagnostic tests for the detection of Infectious Bursal Disease Virus (IBDV) from the three types of bursal samples collected from a recent outbreak in layer and broiler chickens of Gazipur district, Bangladesh. This study compared the degree of sensitivity between Ouchterlony Double Immunodiffusion Test (ODIT)and Reverse Transcription Polymerase Chain Reaction (RT-PCR) for the detection of IBD viral antigen from the bursal samples. A total of 180 field bursal samples (80 broiler and 100 from layer chicken) from suspected IBDV infected dead chickens were collected from 50 different poultry farms. Bursal homogenates were used to detect IBDV using ODIT and RT-PCR. Three types of bursal samples, hemorrhagic bursa (90), edematous bursa (78) and atrophied bursa (12) were selected for the detection of viral antigen. A panel of anti-sera and IBDV specific primer for VP2 gene was used for RT-PCR. The data demonstrated that, out of 180 field samples, 164 (91%) were positive by RT-PCR and 120 (67%) were positive by ODIT. Haemorrhagic bursas were more sensitive compared to oedematous bursas while no virus was detected from the samples of atrophied bursa. This study demonstrated that, RT-PCR was more sensitive and effective diagnostic tool compared to that of ODIT.

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“…Infectious bursal disease (Gumboro, IBD) was first observed in the area of Gumboro, in Delaware, USA [ 1 ]. The virus belongs to the family Birnaviridae, genus Avibirnavirus .…”

Section: Introductionmentioning

confidence: 99%

Assessment of antibody assay methods in determination of prevalence of infectious bursal disease among local chickens and guinea fowls in Kwara state, North Central Nigeria

et al. 2018

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Aim:This study aimed to assess available assay methods for infectious bursal disease (IBD) diagnosis and seromonitoring in local birds. It also sought to know the prevalence of IBD antibodies among local chickens and guinea fowls in Kwara state, North Central Nigeria.Materials and Methods:Sera were obtained from local chickens and guinea fowls and IBD virus (IBDV) antibodies were assayed using enzyme-linked immunosorbent assay (ELISA), indirect hemagglutination (IHA) test, and agar gel immunodiffusion (AGID) test.Results:A total of 265 sera were obtained from local birds during dry and wet seasons. ELISA recorded the highest prevalence of 81.1% (215/265) while IHA and AGID detected IBDV antibodies in 183 (69.1%) and 122 (46%) birds, respectively. Significant differences were established for IBD-positive sera based on the assay method used, bird species, and seasons.Conclusion:This study indicated that ELISA is the most sensitive and reliable assay method while AGID is the least. It also showed that there is a high prevalence of IBDV antibodies among local birds which were not vaccinated, and this implies a high IBDV activity among these bird species in the study area. This may have significant epidemiological implications on the spread of the virus to exotic bird reared in the rural areas on a commercial scale. Thus, this study suggests continuous surveillance, awareness campaign, and advocacy for vaccination of indigenous birds against IBD.

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History of Gumboro (infectious bursal disease) in Pakistan

Cited by 21 publications

References 22 publications

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

Assessment of antibody assay methods in determination of prevalence of infectious bursal disease among local chickens and guinea fowls in Kwara state, North Central Nigeria

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