History of orchid propagation: a mirror of the history of biotechnology

Yam, Tim Wing; Arditti, Joseph

doi:10.1007/s11816-008-0066-3

Cited by 113 publications

(54 citation statements)

References 153 publications

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“…In this study, three media formulations were used as basal media: Vacin and Went (VW), modified MS containing half-strength macro and micro nutrients (½ MS), and Knudson C (KC) (Yam & Arditti, 2009). VW enriched with 10 % (v/v) potato homogenate was used for seed germination, ½ MS was used as basal medium for protocorm development into seedlings in which the leaves were used as the source of explants in experiment I.…”

Section: Medium Preparation and Culture Conditionmentioning

confidence: 99%

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Jainol

Gansau

2017

Agrivita.J.Agr.Sci

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Dimorphorchis lowii is a rare epiphytic orchid endemic to Borneo and very popular due to its ornamental value. For the purpose of mass propagation, procedures for in vitro propagation through callus were developed. The objectives of this study were to determine the effects of plant growth regulators (PGRs) on callus induction from leaf tip explant and the effects of complex additives on PLBs formation and shoot development. The best combination of PGR was 3.0 mg L -1 Thidiazuron (TDZ) with 0.046 mg L -1 NAA supplemented in half-strength medium (½ MS) (Murashige & Skoog, 1962). The percentage of survival and callus formation obtained were 96.0 % ± 19.8 and 52.0 % ± 16.5 respectively. Maximum shoot proliferation from PLBs was observed in Knudson C (KC) medium enriched with 15 % (v/v) coconut water. In this treatment, 10.2 ± 6.2 shoots were produced from one callus explant. Shoots were rooted on KC medium containing 15 % (v/v) coconut water and transferred in a medium mixture containing sphagnum moss, charcoal, brick and coco peat with the ratio of 1:1:1:1. After 2 months being acclimatized in the glasshouse, 78 % of plantlets survived. Histology observations showed that embryogenic callus derived from leaf tip explants might originate from epidermis and mesophyll cells and was capable of developing into complete plantlets.

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Section: Medium Preparation and Culture Conditionmentioning

confidence: 99%

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Jainol

Gansau

2017

Agrivita.J.Agr.Sci

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“…From then on, propagation as well as breeding through hybridization of orchid is done globally. However, hybrids of initial growers could not even have presumed became achievable (Yam and Arditti 2009). The process that involves breeding fresh varieties of Phalaenopsis is rather overwhelmingly extensive and time taking.…”

Section: Introductionmentioning

confidence: 99%

Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Balilashaki

Gantait

Naderi

et al. 2015

Physiol Mol Biol Plants

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We explored the influence of pollination season and maturity of capsule on post-pollination capsule formation and in vitro asymbiotic seed germination, respectively. Three Phalaenopsis orchid hybrids, namely, 'Athens', 'Moscow' and 'Lusaka' flowers were artificially self-pollinated during winter, spring, summer and fall seasons and the impact of the pollination seasons was evident during capsule formation. It was observed that winter was the most suitable season for pollination of all the three Phalanaeopsis hybrids resulting in 80-88 % capsule formation. During summer, the pollination success rate was 24-28 %, but resulted in successful capsule formation. Season of pollination further delimited the germination efficiency of seeds harvested from capsules of variable maturity levels. Invariably, seeds collected from winterpollinated capsules performed best in germination compared to other seasons, for instance, 'Moscow' seeds took less than 14 days to germinate from capsules developed following winter-pollination. Regarding the influence of capsule maturity on seed germination, we observed that seeds derived from 5-month mature capsules, invariably took least time to germinate than that of the 3-month or 7-month in all three hybrids, e.g., for 'Moscow' it was 13.9 days with a maximum of 90.3 % germination.

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“…The practice is done in a controlled laboratory environment which is not susceptible to changing weather conditions, such that production cycles can be planned (Yam and Arditti 2009). Clean, high quality and uniform plants can be produced from otherwise unclean mother plants.…”

Section: Modern Methods Of Seed Yam Productionmentioning

confidence: 99%

Improved propagation methods to raise the productivity of yam (Dioscorea rotundata Poir.)

et al. 2015

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White Guinea yam (Dioscorea rotundata Poir.) is an important staple to millions of people in West Africa. Obtaining good quality planting material for yam cultivation is a major challenge. Multiplication ratios are low, and seed tubers are prone to contamination with pests and pathogens in the traditional systems of production. Some approaches to producing quality seed of yam are as follows: farmers select small whole tubers from a ware crop harvest; stimulate the production of seed tubers by 'milking' ware tubers while the leaves of the plant are still green (double harvest system); cut ware tubers into setts about the same sizes as regular seed tubers; or use the 'Anambra' system where smaller setts are cut and used to produce seed tubers. New methods that have been developed to address some of the challenges of quantity and quality of seed tubers are not yet widely applied, so farmers continue to use traditional methods and save seed from a previous harvest to plant the ware crop. This document presents an overview of traditional and modern methods of seed yam production and gives a perspective for the future. Among the modern methods of seed yam production, only the minisett technique, which uses 25-100 g tuber pieces, is currently used at farmer level, although on a limited scale. While tissue and organ culture techniques are the most rapid methods of multiplying disease free propagules, their limitations include high costs, need for skilled personnel and specialized equipment. The aeroponics and temporary immersion bioreactor methods of producing seed yam are relatively new, and still need more research. To build and sustain a viable seed yam production system, a multiplication scheme is required that combines two or more methods including tissue culture for cleaning the seed stock.

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History of orchid propagation: a mirror of the history of biotechnology

Cited by 113 publications

References 153 publications

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Improved propagation methods to raise the productivity of yam (Dioscorea rotundata Poir.)

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