History of the discoveries of the first human retroviruses: HTLV-1 and HTLV-2

Gallo, Robert C.

doi:10.1038/sj.onc.1208980

Cited by 163 publications

(111 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HTLV-1, the first human retrovirus discovered, is the causative agent of many ailments, most notably adult T cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis (Gallo, 2005;Poiesz et al, 1981). Despite over 10 million people infected worldwide with HTLV-1, the mechanisms of disease pathogenesis are not yet completely understood.…”

Section: Nih Public Accessmentioning

confidence: 99%

A novel high throughput quantum dot-based fluorescence assay for quantitation of virus binding and attachment

Kampani

Quann

Ahuja

et al. 2007

Journal of Virological Methods

View full text Add to dashboard Cite

Quantum dots (QDots) are fluorescent semiconductor nanocrystals with a narrow emission spectrum, high quantum yield, and excellent photostability. These unique properties of QDots have been utilized to develop a fluorescent binding assay using biotinylated human T cell leukemia virus type 1 (biot-HTLV-1) conjugated with streptavidin-coated Qdots that enabled both qualitative and quantitative analyses of viral binding. The specificity and linearity of the assay was demonstrated utilizing T cells, the primary HTLV-1-susceptible cell population. Furthermore, differential binding of HTLV-1 was analyzed in additional cell types of clinical relevance including primary CD4 + and CD8 + T cells, dendritic cells (DCs), monocytes, bone marrow progenitor cells, and epithelial cells. DCs exhibited maximum binding affinity when compared to other examined cell types except the Jurkat and SUP-T1 T cell lines. Finally, blocking antibodies directed against a putative HTLV-1 receptor on DCs; DC-SIGN (dendritic cell-specific ICAM-3-grabbing non-integrin), were utilized to study the inhibition of HTLV-1 binding to target cells. Overall, these results demonstrated that this novel high throughput assay can be utilized to study the binding of a biotinylated virus and has implications for screening of viral binding inhibitors as well as host membrane proteins that may serve as receptors for viral entry. KeywordsHTLV-1; quantum dot; viral binding assay 1.IntroductionViral binding and attachment to a host cell membrane, while seemingly simplistic, is a complex area of research for a wide range of viruses. It is often viewed as the first step in infection, whereby a virion is able to attach to a target cell, fuse to the cell membrane, and deliver the contents of the capsid to the cytoplasm of the newly infected cell. The exact mechanism of binding to a host cell varies between viruses and is usually determined by the composition of attachment proteins located within the viral and cellular membranes. The population of cells † A part of this work was presented during 7th International Symposium on NeuroVirology (ISNV), Philadelphia, PA, USA, May 30 - Street, Philadelphia, PA 19102, USA, Tel.: 215-762-8586; Fax: 215-762-1955, E-mail: pjain@drexelmed.edu, Web site: http://www.drexelmed.edu/ Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. (Dhawan et al., 1991;Inghirami et al., 1988). Occasionally, radioactive labels are also utilized for the quantitative estimation of binding (Hubbard, 2003). However, organic fluorophores conventionally used for labeling nucleotides and proteins have poor pho...

show abstract

Section: Nih Public Accessmentioning

confidence: 99%

A novel high throughput quantum dot-based fluorescence assay for quantitation of virus binding and attachment

Kampani

Quann

Ahuja

et al. 2007

Journal of Virological Methods

View full text Add to dashboard Cite

show abstract

“…H uman T-cell leukemia virus type 1 (HTLV-1) causes a malignancy named adult T-cell leukemia (ATL) and several inflammatory diseases including HTLV-1-associated myelopathy/ tropical spastic paraparesis (HAM/TSP) (1,2). HTLV-1 encodes a critical transactivator, Tax, that induces the activation and subsequent clonal expansion of infected T cells in vivo (2,3).…”

mentioning

confidence: 99%

TCF1 and LEF1 act as T-cell intrinsic HTLV-1 antagonists by targeting Tax

Ma¹,

Yasunaga²,

Akari³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…En 1982, Yoshida y colaboradores (11) identificaron un virus tipo C como causa de una enfermedad previamente descrita en 1977 en Japón, conocida como leucemia de células T en el adulto (LTA); tiempo después se demostró que este virus y el HTLV tenían secuencias genómicas idénticas, por lo que recibió el nombre de "HTLV tipo 1" y su enfermedad asociada "leucemia/linfoma de células T en el adulto" (12). Posteriormente, se describieron tres tipos más de HTLV, de los cuales HTLV-1 y HTLV-2 se conocen como agentes etiológicos de enfermedades en seres humanos, mientras que HTLV-3 y HTLV-4 se han asociado con enfermedades en animales (13,14).…”

Section: Historiaunclassified