HIV-1 genotyping tropism profile in an HIV-positive population throughout the Russian Federation

Lopatukhin, Alexey; Kireev, Dmitry; Куевда, Д А; Pokrovskaya, Anastasia; Tsyganova, G. M.; Korovina, Galina; Pinsker, Alexander; Дементьева, Наталья Евгеньевна; Sizova, N V; Peksheva, O.Yu.; Zaytseva, Natalia; Носов, Н. Н.; Urazov, Nail; Герасимов, В. В.; Ermolinskaya, Natalia; Герасимова, Н. А.; Сандырева, Т П; Волова, Л Ю; Grezina, Lilia; Коломеец, А. Н.; Сергеева, И. В.; Нешумаев, Д. А.; Boyko, A. L.; Котова, В. О.; Балахонцева, Л. А.; Kolpakov, Dmitry; Shemshura, Andrey; Saukhat, Sergey; Букин, Е. К.; Polyakov, A.; Kaiser, Rolf; Shipulin, German A.; Pokrovsky, V I

doi:10.1080/2331205x.2017.1311470

Cited by 2 publications

(3 citation statements)

References 17 publications

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“…The analysis of the resistant HIV-1 transmission in Krasnoyarsk Krai revealed infection with HIV-1 carrying mutations associated with the development of the virus resistance to PI, NRTI, and NNRTI in 8.2% of studied cases. Described HIV-1 mutations and the degree of their incidence and analysis of HIV-1 tropism correspond well with data obtained by other researchers for other Russian regions [ 15 , 23 , 45 – 49 ]. Most frequently detected was substitution K103N (3/10), which is DR-mutation to NNRTI.…”

Section: Discussionsupporting

confidence: 86%

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Genetic Diversity of HIV-1 in Krasnoyarsk Krai: Area with High Levels of HIV-1 Recombination in Russia

Maksimenko

Gashnikova

et al. 2020

BioMed Research International

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More than a quarter of HIV-infected individuals registered in Russia live in Siberia. Unlike Central Russia where HIV-1 subtype A6 is predominant, in most Siberian regions since 2012, a new HIV-1 CRF63_02A1 genetic variant has spread, with the share of this variant attaining 75–85% among newly identified HIV cases. Krasnoyarsk Krai is considered to be a high-risk territory according to morbidity rate and HIV infection incidence among the population. The current paper aims to study the molecular epidemiologic characteristics of HIV-1 spreading in Krasnoyarsk Krai. Phylogenetic and recombination analyses of pol (PR-RT, IN) and env regions of the virus were used for genotyping 159 HIV-1 isolated in Krasnoyarsk Krai. 57.2% of the isolates belonged to subtype A (A6) specific to Russia, 12.6% to CRF63_02A1, and 0.6% to CRF02_AGСА, and in 29.6% HIV-1 URFs were detected, including URF63/А (23.9%), URFА/В (4.4%), and URF02/А (1.3%). In 6 of 7, HIV-1 URFА/В identical recombination model was detected; the origin of 38 URF63/А was proven to be the result of individual recombination events. Since 2015, a share of the population with newly diagnosed HIV who were infected with HIV-1 URF reached an exceptionally high rate of 38.6%. As distinct from adjacent Siberian regions, the HIV-1 CRF63_02A1 prevalence rate in Krasnoyarsk Krai is within 16%; however, the increased contribution of new HIV-1 into the regional epidemic development was observed due to the recombination of viruses of subtypes А, В, and CRF63_02A1. The difference between the described molecular epidemiologic picture in Krasnoyarsk Krai and in adjacent areas is likely caused by differences in predominant routes of HIV transmission and by more recent HIV-1 CRF63_02A1 transmission in the PWID group, which had a high prevalence of HIV-1 subtype A by the time of the new virus transmission, resulting in increased possibility of coinfection with various HIV-1 genetic variants.

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Section: Discussionsupporting

confidence: 86%

“…It was shown that random shuffling of HIV genetic material not only increases viral diversity but also enables the virus to evade the response of the human immune system [ 51 ]. The increase in circulating HIV-1 genetic diversity is a general characteristic for many current territorial epidemics [ 43 , 49 , 52 – 56 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of HIV-1 in Krasnoyarsk Krai: Area with High Levels of HIV-1 Recombination in Russia

Maksimenko

Gashnikova

et al. 2020

BioMed Research International

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“…Additionally, there is also some correlation with HIV-1 genetic variants. R5 viruses are more often detected in subtype A, regardless of the stage of the disease [20], [21], [22].…”

Section: Introductionmentioning

confidence: 99%

HIV Coreceptors Related Genes (CCR5 and CXCR4) Promoter Methylation: Original Research

Esman,

Salamaikina,

Vinokurov

et al. 2024

Preprint

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The persistence of human immunodeficiency virus (HIV) in viral reservoirs is a topical issue. There are many factors that can potentially influence this process. Epigenetic alterations according to the literature could be such factors. In this study, we present a detailed description of gene promoters research and development techniques for determining the methylation level of CpG loci in these promoters based on Sanger sequencing and pyrosequencing. The presented methods could be used for measuring the methylation levels in other research.A total of 51 samples of biological material from 17 patients at three visits were used in this study. Applying the developed techniques, we found that the CpG loci of theCXCR4gene promoter were not methylated. For CpG loci of theCCR5gene promoter no change in methylation levels was observed visit to visit. CpG 1 methylation level ranged from 33% to 53%, CpG 2 - from 4.4% to 11%, CpG 3 - from 0 to 42% and CpG 4 - from 1% to 21%. These values were derived from measurements of C-peaks in sequences.Key ContributionThe breakthroughs or highlights of the manuscript. Authors can write one or two sentences to describe the most important part of the paper.

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HIV-1 genotyping tropism profile in an HIV-positive population throughout the Russian Federation

Cited by 2 publications

References 17 publications

Genetic Diversity of HIV-1 in Krasnoyarsk Krai: Area with High Levels of HIV-1 Recombination in Russia

Genetic Diversity of HIV-1 in Krasnoyarsk Krai: Area with High Levels of HIV-1 Recombination in Russia

HIV Coreceptors Related Genes (CCR5 and CXCR4) Promoter Methylation: Original Research

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