HIV-1 infection using dried blood spots can be confirmed by Bio-Rad Geenius™ HIV 1/2 confirmatory assay

McPhee, Carolina Fernández; Álvarez, Patrícia; Prieto, Luís; Obiang, Jacinta; Avedillo, Pedro; Vargas, Antonio; Rojo, Pablo; Abad, Carlota; Ramos, José Tomás; Holguín, África

doi:10.1016/j.jcv.2014.12.018

Cited by 14 publications

(13 citation statements)

References 10 publications

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“…HIV diagnosis was firstly performed in DRC using rapid serological tests: Determine™ HIV-1/2 Ag/Ab (Alere), Double-Check Gold HIV 1&2 (Orgenics) and Uni-Gold HIV (Trinity Biotech) from 18-months old and by Biomerieux 4th generation immunoassay VIDAS® HIV Duo Ultra or exceptionally by molecular Abbott real-time HIV-1 Qualitative in infants under 18-months old. In Madrid, Spain, HIV serological status in the 85 children was confirmed with BioRad Geenius TM HIV-1/2 confirmatory assay using one DBS dot per patient, as previously reported 33 . All HIV seropositive and undetermined pediatric DBS by Geenius were then tested by POC Cepheid Xpert Qual (Xpert Qual), which provides a binary “detected”/“not detected” result 34 .…”

Section: Methodsmentioning

confidence: 73%

“…A correct early HIV diagnosis is also essential, since false positive HIV tests might result in unnecessary antiretroviral treatment and psychological distress in falsely diagnosed individuals and families 50 . The use of DBS has been proposed as an alternative sample to plasma/serum, easier to be collected, stored and shipped, very convenient in limited resource countries 15,33,37,51 . The aim of this study was to evaluate the performance of two POC HIV-1 assays (Xpert-VL and Xpert Qual) for HIV detection and/or quantification using DBS in the DRC, a country with a high HIV-1 diversity including a high prevalence of complex recombinants, mainly URFs.…”

Section: Discussionmentioning

confidence: 99%

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Utility Of POC Xpert HIV-1 Tests For Detection-Quantification Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congo

Rubio-Garrido

Ndarabu

Reina

et al. 2019

Sci Rep

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Point-of-Care (POC) molecular assays improve HIV infant diagnosis and viral load (VL) quantification in resource-limited settings. We evaluated POC performance in Kinshasa (Democratic Republic of Congo), with high diversity of HIV-1 recombinants. In 2016, 160 dried blood samples (DBS) were collected from 85 children (60 HIV−, 18 HIV+, 7 HIV-exposed) and 75 HIV+ adults (65 treated, 10 naive) at Monkole Hospital (Kinshasa). We compared viraemia with Cepheid-POC-Xpert-HIV-1VL and the non-POC-COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1-Testv2 in all HIV+, carrying 72.4%/7.2% HIV-1 unique/complex recombinant forms (URF/CRF). HIV-1 infection was confirmed in 14 HIV+ children by Cepheid-POC-Xpert-HIV-1Qual and in 70 HIV+ adults by both Xpert-VL and Roche-VL, identifying 8 false HIV+ diagnosis performed in DRC (4 adults, 4 children). HIV-1 was detected in 95.2% and 97.6% of 84 HIV+ samples by Xpert-VL and Roche-VL, respectively. Most (92.9%) HIV+ children presented detectable viraemia by both VL assays and 74.3% or 72.8% of 70 HIV+ adults by Xpert or Roche, respectively. Both VL assays presented high correlation (R 2 = 0.89), but showing clinical relevant ≥0.5 log VL differences in 15.4% of 78 cases with VL within quantification range by both assays. This is the first study confirming the utility of Xpert HIV-1 tests for detection-quantification of complex recombinants currently circulating in Kinshasa.

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Section: Methodsmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Utility Of POC Xpert HIV-1 Tests For Detection-Quantification Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congo

Rubio-Garrido

Ndarabu

Reina

et al. 2019

Sci Rep

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“…In addition to screening for metabolic disorders, DBS cards have been utilised for human immunodeficiency virus (HIV) screening, laboratory quality control, drug testing and detection of pathogens in diverse sample types, including blood and dried plasma spots [6,7]. Numerous studies have also demonstrated that antibodies can be detected on DBS, such as in the prospective cohort study of congenital cytomegalovirus [8] and HIV infection [9]. DBS samples are cost-effective as easily portable equipment (i.e.…”

Section: Introductionmentioning

confidence: 99%

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

et al. 2021

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Background Increasingly, vaccine efficacy studies are being recommended in low-and-middle-income countries (LMIC), yet often facilities are unavailable to take and store infant blood samples correctly. Dried blood spots (DBS), are useful for collecting blood from infants for diagnostic purposes, especially in low-income settings, as the amount of blood required is miniscule and no refrigeration is required. Little is known about their utility for antibody studies in children. This systematic review aims to investigate the correlation of antibody concentrations against infectious diseases in DBS in comparison to serum or plasma samples that might inform their use in vaccine clinical trials. Methods and findings We searched MEDLINE, Embase and the Cochrane library for relevant studies between January 1990 to October 2020 with no language restriction, using PRISMA guidelines, investigating the correlation between antibody concentrations in DBS and serum or plasma samples, and the effect of storage temperature on DBS diagnostic performance. We included 40 studies in this systematic review. The antibody concentration in DBS and serum/plasma samples reported a good pooled correlation, (r2 = 0.86 (ranged 0.43 to 1.00)). Ten studies described a decline of antibody after 28 days at room temperature compared to optimal storage at -20°C, where antibodies were stable for up to 200 days. There were only five studies of anti-bacterial antibodies. Conclusions There is a good correlation between antibody concentrations in DBS and serum/plasma samples, supporting the wider use of DBS in vaccine and sero-epidemiological studies, but there is limited data on anti-bacterial antibodies. The correct storage of DBS is critical and may be a consideration for longer term storage.

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“…by molecular Abbott real-time HIV-1 Qualitative in infants under the age of 18 months old. In Madrid, Spain, HIV serological status in the 85 children was confirmed with the BioRad Geenius TM HIV-1/2 confirmatory assay using one DBS spot per patient, as previously reported (22). All HIV seropositive and undetermined pediatric DBS by Geenius were then tested by molecular POC test Cepheid Xpert Qual (Xpert Qual), which provides a binary "detected"/"not detected" result (23).…”

Section: Hiv Diagnosis and Viremia Quantificationmentioning

confidence: 99%

Dried Blood Specimens as an Alternative Specimen for Immune Response Monitoring During HIV Infection: A Proof of Concept and Simple Method in a Pediatric Cohort

et al. 2021

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Programs to prevent mother-to-child HIV transmission do not reduce the number of infants exposed during pregnancy and breastfeeding. HIV-exposed but uninfected children (HEU) present higher risk of morbidity and mortality than HIV-unexposed and uninfected children (UU). In this line, the study of immune biomarkers in HIV could improve prediction of disease progression, allowing to diminish comorbidity risk. Dried blood specimens (DBS) are an alternative to serum for collecting and transporting samples in countries with limited infrastructure and especially interesting for groups such as pediatrics, where obtaining a high sample volume is challenging. This study explores the usefulness of DBS for immune profile monitoring in samples from 30 children under clinical follow-up in Kinshasa: 10 HIV-infected (HIV+), 10 HEU, and 10 UU. We have measured the gene expression levels of 12 immune and inflammatory markers (CD14, IL-6, TNFα, HVEM, B7.1, HIF-1α, Siglec-10, IRAK-M, CD163, B7H5, PD-L1, and Galectin-9) in DBS samples by reverse transcription of total RNA and RT-qPCR. Principal component analysis, Kruskal–Wallis test, and Mann–Whitney test were performed in order to study group differences. HIV+ children presented significantly higher levels of seven biomarkers (CD14, IL-6 HVEM, B7.1, Siglec-10, HIF-1α, and CD163) than the UU group. In HEU, we found seven biomarkers significantly elevated (CD14, IL-6, HVEM, B7.1, Siglec-10, HIF-1α, and IRAK-M) vs. UU. Six biomarkers (CD14, IL-6, HVEM, B7.1, Siglec-10, and HIF-1α) showed a significantly higher expression in both HIV+ and HEU vs. UU, with HVEM and CD14 being significantly overexpressed among HIV+ vs. HEU. Our data reveal the utility of DBS for immune response monitoring. Moreover, significant differences in specific biomarker expression across groups strongly suggest the effect of HIV infection and/or HIV exposure on these immune biomarkers' expressions.

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HIV-1 infection using dried blood spots can be confirmed by Bio-Rad Geenius™ HIV 1/2 confirmatory assay

Cited by 14 publications

References 10 publications

Utility Of POC Xpert HIV-1 Tests For Detection-Quantification Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congo

Utility Of POC Xpert HIV-1 Tests For Detection-Quantification Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congo

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

Dried Blood Specimens as an Alternative Specimen for Immune Response Monitoring During HIV Infection: A Proof of Concept and Simple Method in a Pediatric Cohort

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