HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses

Abstract

Background: Conserved neutralizing epitopes are considered to be a key role for eliciting broadly neutralizing antibody (NAb). Previously, two conserved neutralizing epitopes of HIV-1 CRF01_AE envelope were identified at amino acid 93-112 of the C1 (C1E) and at 218-239 of the C2 (C2E) regions. To access the potency of antibody directed against conserved epitopes, a monoclonal antibody (MAb) specific to the C2E region was developed and characterized.

Designing of novel antigenic peptide cocktail for the detection of antibodies to HIV-1/2 by ELISA

“…To overcome this problem, diagnostic methods employing shorter HIV antigens were developed. Empirical methods to identify peptides corresponding to unique and highly conserved epitopes of the HIV viruses have been identified that helped in pinpointing amino acid sequences that are more likely to be exposed on the surface of native proteins (Sreepian et al, 2009;Nyambi et al, 2000).…”

Evolution of antibody landscape and viral envelope escape in an HIV-1 CRF02_AG infected patient with 4E10-like antibodies

“…Together with the fact that the C1 region is known to be less variable [ 43 ], the C1 epitopes are interesting targets for neutralizing antibodies. Previously, a report by Sreepian et al , described the presence of antibodies against the C1 region in a subtype CRF01_AE infected HIV-1 individual, however, immunization studies performed with C1 epitopes did not result in neutralizing antibodies [ 44 , 45 ]. The role of the C1 antibodies in the neutralization capacity of ITM4 should be further analyzed.…”