2017
DOI: 10.1007/s00430-017-0494-1
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HIV-1 persistent viremia is frequently followed by episodes of low-level viremia

Abstract: After the start of antiretroviral therapy (ART), plasma HIV-RNA levels should fall below the limit of detection (LOD) within 24 weeks. Hence, the prolonged decline of HIV-RNA after ART initiation is defined as persistent viremia (PV). In this retrospective study, we analyzed factors associated with PV. Next-generation sequencing of viral RNA/DNA was performed to study viral evolution and the emergence of drug-resistance mutations in HIV-infected patients with PV (n = 20). In addition, HIV-DNA species, immunolo… Show more

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Cited by 17 publications
(14 citation statements)
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“…Our study was not well suited to evaluate this effect, as rifampin was co-administered with NVP in only three participants. Suboptimal antiretroviral concentrations at times of LLV have been reported from other studies [ 48 50 ], suggesting that poor-adherence or drug–drug interactions could be a cause of LLV. However, given the generally therapeutic levels in our study, this was not likely a cause of LLV.…”
Section: Discussionmentioning
confidence: 71%
“…Our study was not well suited to evaluate this effect, as rifampin was co-administered with NVP in only three participants. Suboptimal antiretroviral concentrations at times of LLV have been reported from other studies [ 48 50 ], suggesting that poor-adherence or drug–drug interactions could be a cause of LLV. However, given the generally therapeutic levels in our study, this was not likely a cause of LLV.…”
Section: Discussionmentioning
confidence: 71%
“…By using an input volume of 100 µl plasma the detection limit was 300 copies/ml. Total HIV-1 DNA was quantified performing a pre-PCR followed by a probe-based real-time PCR approach as described previously 19,20 . Briefly, genomic DNA was isolated from splenic CD4 + cells using QIAamp DNA Mini Kit (Qiagen).…”
Section: Methodsmentioning
confidence: 99%
“…TaqMan probes for CD3 and HIV-1-DNA quantification were dually labelled with YAK-BHQ-1 and 6-FAM-BHQ-1, respectively. Standard curves were generated with plasmid DNA templates harboring the corresponding amplicon regions or genomic DNA of HIV-1 LTR-harbouring cells for the quantification of integrated provirus 20 . Determinations of HIV infection levels in plasma for group assignments were done by p24 ELISA (Advanced Bioscience Laboratories, Rockville MD).…”
Section: Methodsmentioning
confidence: 99%
“…Depending on the available sample volume, the detection limit was 375 cp/mL during follow-up or 150 cp/mL at the time of euthanasia. For the quantification of HIV DNA, genomic DNA of splenocytes was isolated using QIAamp DNA blood mini kit (Qiagen) and subjected to a probebased real-time PCR approach, as previously described 71,72 . Briefly, HIV-specific and control (hCD3) DNA sequences were pre-amplified (12 cycles) in a TProfessional TRIO Thermocycler…”
Section: Measurement Of Hiv In Tko-blt Humanized Micementioning
confidence: 99%