HIV-1 Proteins gp120 and Tat Promote Epithelial-Mesenchymal Transition and Invasiveness of HPV-Positive and HPV-Negative Neoplastic Genital and Oral Epithelial Cells

Lien, Kathy; Wasima, Mayer; Herrera, Rossana; Padilla, Nicole T; Cai, Xiaodan; Lin, Vicky; Pholcharoenchit, Rangsimon; Palefsky, Joel M.; Tugizov, Sharof M.

doi:10.1128/spectrum.03622-22

Cited by 12 publications

(11 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, HIV 1 and its virulence proteins (gp120 and tat) exposure to the HPV 16 immortalised cell lines advanced the EMT transition and further metastasis. The same results were observed in the HPV‐negative cell line 98 . The correlation between OSCC and HIV‐HPV confection is not studied.…”

Section: Hiv and Hpv Virus Impact On Osccsupporting

confidence: 53%

See 1 more Smart Citation

A systematic review on the molecular and clinical association between Human Papillomavirus and Human Immunodeficiency Virus co‐infection in Head, Neck and Oral squamous cell carcinoma

Chakraborty

Ramasubbu

Banerjee

et al. 2023

Reviews in Medical Virology

View full text Add to dashboard Cite

Head and neck cancer, one of the most commonly prevalent malignancies globally is a complex category of tumours that comprises cancers of the oral cavity, pharynx, and larynx. A specific subgroup of such cancers has been found with some unique chromosomal, therapeutic, and epidemiologic traits with the possibility of affecting via co‐infection. About 25% of all head and neck cancers in the population are human papillomavirus infection (HPV)‐associated, typically developing in the oropharynx, which comprises the tonsils. In the period of efficient combined antiviral treatment, HPV‐positive oral cancers are also becoming a significant contributor to illness and fatality for Human Immunodeficiency Virus (HIV)‐infected persons. Although the prevalence and historical background of oral HPV transmission are not thoroughly understood, it seems likely that oral HPV transmission is relatively frequent in HIV‐infected people when compared to the overall population. Therefore, there is a need to understand the mechanisms leading to this co‐infection, as there is very little research related to that. Hence, this study mainly focus on the therapeutical and biomedical analysis of HPV and HIV co‐infection in the above‐mentioned cancer, including oral squamous cell carcinoma.

show abstract

Section: Hiv and Hpv Virus Impact On Osccsupporting

confidence: 53%

“…The same results were observed in the HPV-negative cell line. 98 The correlation between OSCC and HIV-HPV confection is not studied. The molecular mechanism must be explored to determine possible treatment options.…”

Section: Hiv and Hpv Virus Impact On Osccmentioning

confidence: 99%

A systematic review on the molecular and clinical association between Human Papillomavirus and Human Immunodeficiency Virus co‐infection in Head, Neck and Oral squamous cell carcinoma

Chakraborty

Ramasubbu

Banerjee

et al. 2023

Reviews in Medical Virology

View full text Add to dashboard Cite

show abstract

“…Increased production of TGF-β which promotes immunosuppression was noted in HIV and also in simian immunodeficiency virus (SIV) infection. An enhanced intestinal TGF-β/Smad-dependent signaling in SIV-infected rhesus macaques was reported by Boby et al (2021) [ 233 ].…”

Section: Roles Of Tgf β In Viral Infection At the Non-maternal–fetal ...mentioning

confidence: 78%

Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives

Trinh

Pham

Takada

et al. 2023

IJMS

View full text Add to dashboard Cite

Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic growth factor playing various roles in the human body including cell growth and development. More functions of TGF-β1 have been discovered, especially its roles in viral infection. TGF-β1 is abundant at the maternal–fetal interface during pregnancy and plays an important function in immune tolerance, an essential key factor for pregnancy success. It plays some critical roles in viral infection in pregnancy, such as its effects on the infection and replication of human cytomegalovirus in syncytiotrophoblasts. Interestingly, its role in the enhancement of Zika virus (ZIKV) infection and replication in first-trimester trophoblasts has recently been reported. The above up-to-date findings have opened one of the promising approaches to studying the mechanisms of viral infection during pregnancy with links to corresponding congenital syndromes. In this article, we review our current and recent advances in understanding the roles of TGF-β1 in viral infection. Our discussion focuses on viral infection during pregnancy, especially in the first trimester. We highlight the mutual roles of viral infection and TGF-β1 in specific contexts and possible functions of the Smad pathway in viral infection, with a special note on ZIKV infection. In addition, we discuss promising approaches to performing further studies on this topic.

show abstract

“…Both gp120 and RT increase the expression of HPV16 E6 in HPV16 infected immortalized and/or completely transformed epithelial cells, while other HIV-1 proteins, such as capsid protein p24, had no effect [ 2 ]. Furthermore, the prolonged interaction of HIV-1 proteins gp120 and tat and cell-free HIV-1 virions with HPV16-immortalized anal, cervical, and oral epithelial cells was found to stimulate EMT and increase the invasiveness of HPV16-infected cells [ 47 , 48 ]. Overall, these data indicate that the presence of HIV-1 and/or its antigens in HPV16-infected neoplastic cells may potentiate HPV-associated tumorigenicity, making HIV-1 infection an integral part of the process of HPV-associated tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

HIV-1 Reverse Transcriptase Expression in HPV16-Infected Epidermoid Carcinoma Cells Alters E6 Expression and Cellular Metabolism, and Induces a Hybrid Epithelial/Mesenchymal Cell Phenotype

Zhitkevich,

Bayurova,

Avdoshina

et al. 2024

Viruses

Self Cite

View full text Add to dashboard Cite

The high incidence of epithelial malignancies in HIV-1 infected individuals is associated with co-infection with oncogenic viruses, such as high-risk human papillomaviruses (HR HPVs), mostly HPV16. The molecular mechanisms underlying the HIV-1-associated increase in epithelial malignancies are not fully understood. A collaboration between HIV-1 and HR HPVs in the malignant transformation of epithelial cells has long been anticipated. Here, we delineated the effects of HIV-1 reverse transcriptase on the in vitro and in vivo properties of HPV16-infected cervical cancer cells. A human cervical carcinoma cell line infected with HPV16 (Ca Ski) was made to express HIV-1 reverse transcriptase (RT) by lentiviral transduction. The levels of the mRNA of the E6 isoforms and of the factors characteristic to the epithelial/mesenchymal transition were assessed by real-time RT-PCR. The parameters of glycolysis and mitochondrial respiration were determined using Seahorse technology. RT expressing Ca Ski subclones were assessed for the capacity to form tumors in nude mice. RT expression increased the expression of the E6*I isoform, modulated the expression of E-CADHERIN and VIMENTIN, indicating the presence of a hybrid epithelial/mesenchymal phenotype, enhanced glycolysis, and inhibited mitochondrial respiration. In addition, the expression of RT induced phenotypic alterations impacting cell motility, clonogenic activity, and the capacity of Ca Ski cells to form tumors in nude mice. These findings suggest that HIV-RT, a multifunctional protein, affects HPV16-induced oncogenesis, which is achieved through modulation of the expression of the E6 oncoprotein. These results highlight a complex interplay between HIV antigens and HPV oncoproteins potentiating the malignant transformation of epithelial cells.

show abstract

HIV-1 Proteins gp120 and Tat Promote Epithelial-Mesenchymal Transition and Invasiveness of HPV-Positive and HPV-Negative Neoplastic Genital and Oral Epithelial Cells

Cited by 12 publications

References 95 publications

A systematic review on the molecular and clinical association between Human Papillomavirus and Human Immunodeficiency Virus co‐infection in Head, Neck and Oral squamous cell carcinoma

A systematic review on the molecular and clinical association between Human Papillomavirus and Human Immunodeficiency Virus co‐infection in Head, Neck and Oral squamous cell carcinoma

Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives

HIV-1 Reverse Transcriptase Expression in HPV16-Infected Epidermoid Carcinoma Cells Alters E6 Expression and Cellular Metabolism, and Induces a Hybrid Epithelial/Mesenchymal Cell Phenotype

Contact Info

Product

Resources

About