HIV-1 single transcription start site mutants display complementary replication functions that are restored by reversion

Abstract

HIV-1 transcription initiates at two positions, generating RNAs with eithercap1G orcap3G 5′ ends. The replication fates of these RNAs differ, with viral particles encapsidating almost exclusivelycap1G RNAs andcap3G RNAs retained in cells where they are enriched on polysomes and among spliced viral RNAs. Here, we studied replication properties of virus promoter mutants that produced only one RNA 5′ isoform or the other: separately, in combination, and during spreading infection. Results showed that either single start RNA could serve as both mRNA and genomic RNA when present as the only form in cells, althoughcap3G RNA was more efficiently translated and spliced whilecap1G RNA was packaged into nascent virions slightly better than RNAs from the parental virus. When co-expressed from separate vectors,cap1G RNA was preferentially packaged into virions. During spreading infectioncap1G-only virus displayed only minor defects butcap3G-only virus showed severe replication delays in both the highly permissive MT-4 cell line and in primary human CD4+ T cells. Passage ofcap3G-only virus yielded revertants that replicated as well as the twinned (cap1G+cap3G) transcription start site parent. These revertants displayed restored packaging and splicing levels and had regained multiple transcription start site use.