HIV-1-specific B cell activation. A major constituent of spontaneous B cell activation during HIV-1 infection.

Abstract

B cell activation is a well known consequence of HIV-1 infection, and seropositive subjects show high numbers of spontaneously activated Ig-secreting cells in circulation. To better define the importance of the HIV-1-specific response in this phenomenon, we first studied whether in vitro spontaneous anti-HIV-1 antibody production was accompanied by reactivation of memory B lymphocytes. Unstimulated PBL from HIV-1-infected individuals with prior history of hepatitis B and/or EBV infection did not consistently show spontaneous in vitro synthesis of anti-hepatitis B core Ag or anti-EBV antibodies; in addition, PWM-induced synthesis of anti-hepatitis B virus and anti-EBV antibodies was decreased compared to HIV-1-seronegative subjects. Moreover, in comparing the frequencies of activated HIV-1-specific B cell precursors and activated Ig-secreting precursors in limiting dilution experiments, a sizable fraction (20 to 40%) of circulating cells spontaneously secreting Ig produced antibody against HIV-1 determinants. The ratio between the two frequencies fitted in very well with the amount of Ig removed from unstimulated culture supernatants after HIV-1-specific antibody absorption with solid-phase HIV-1. These findings indicate that B cell activation during HIV-1 infection is mainly oriented toward a specific response to HIV-1 determinants; the possible relevance of this phenomenon to lymphomagenesis in AIDS patients is discussed.

Sex, Age, and Risk Group Variations among Individuals Infected with HIV, HTLV-1, and HTLV-2: Review of Data Records (1983–2017) from a Public Health Laboratory in São Paulo, Brazil

“…Considering the literature that highlights the polyclonal activation of B lymphocytes in HIV infection [24], along with the spontaneous secretion of HIV antibodies in the supernatant of prolonged lymphocyte cultures [25], we developed an alternative method for use with children. This technique is referred to as in vitro-induced antibody production (IVIAP).…”

Desafios e contribuição do Laboratório de Imunologia Celular do Instituto Adolfo Lutz de São Paulo nos primeiros anos da epidemia de AIDS no Brasil

“…Utilizando informações de literatura que descreviam ativação policlonal de linfócitos B na AIDS e secreção espontânea de anticorpos anti-HIV em sobrenadante de cultura de células mononucleares (CMNs) do sangue periférico de longa duração de pacientes infectados, 16,17 decidiu-se em 1999, investir na possibilidade de utilizar a produção de anticorpos in vitro como ferramenta para o diagnóstico de infecção perinatal pelo HIV. A técnica denominada in vitro antibody production (IVAP) levava cerca de oito dias para produzir anticorpos em quantidade suficiente para serem detectados em ensaios sorológicos, apesar de esta produção ter início nas primeiras 24h de cultura (Figura 7).…”