HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

Muciaccia, Barbara; Corallini, Serena; Vicini, Elena; Padula, Fabrizio; Gandini, L.; Liuzzi, Giuseppina; Lenzi, Andrea; Stefanini, Mario

doi:10.1093/humrep/dem288

Cited by 55 publications

(32 citation statements)

References 85 publications

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“…These results agree with these found by only one author [28], to the best of our knowledge, who used the same slide technique to analyze sperm DNA fragmentation in HIV-1 infected patients. In this previous study, sperm DNA fragmentation rates were higher than ours, but no details on sperm parameters were indicated.…”

Section: Fragmentation Rate and Viral Infectionsupporting

confidence: 92%

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Frainais

Vialard

Rougier

et al. 2010

J Assist Reprod Genet

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Introduction According french legislation, sperm freezing/ thawing procedures are used to prevent ART contaminations in couple with HIV-1 infected men. We determined sperm nuclear fragmentation rate before and after selection and freezing/thawing in HIV-1 14 patients.Methods Two groups of patients were studied: 20 control patients with normal sperm (group 1) and without viral infection and 20 fertile treated HIV-1 patients (group 2). DNA fragmentation was evaluated using terminal uridine nick end labeling, before and after gradient selection, and after cryopreservation and thawing procedures. Results DNA fragmentation rates in fresh semen were increased in HIV patients (6.38% vs 3.39%) (p<0.05) compared with control patients. After sperm migration, fragmentation rates were significantly lower (p<0.0001) in the two groups compared with fresh sperm rates. After freezing/thawing, values were similar to those of fresh semen with an increased rate (p<0.01) for HIV-1 patients, with respectively 3.40% and 5.18% rates in control and infected patients. HIV-1-infected patients treated by antiretroviral therapy showed a significant increase in sperm DNA fragmentation in fresh sperm and also after freezing/ thawing procedures, but these two fragmentation rates were not significantly different. Conclusion So, freezing/thawing procedures do not seem to impair sperm DNA and preserve probability of conception for couples with HIV-1 infected men.

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Section: Fragmentation Rate and Viral Infectionsupporting

confidence: 92%

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Frainais

Vialard

Rougier

et al. 2010

J Assist Reprod Genet

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“…By reviewing studies conducted in raw semen from similar groups Muciaccia et al 2007;O'Flaherty et al 2008;Cohen-Bacrie et al 2009;Tunc et al 2008;Martin et al 2005Formaldeyde d Muratori et al 2003Caglar et al 2007;Tarozzi et al 2009;Domínguez-Fandos et al 2007;Young et al 2003;Avendañ o et al 2009a and2009b;Stronati et al 2006;Ramos et al 2008;Tesarik et al 2004 Permeabilization with detergents Absent Martin et al 2005;O'Flaherty et al 2008;Young et al 2003;Said et al 2006Present e Chohan et al 2006Aoki et al 2006;Caglar et al 2007;Varum et al 2007;Muratori et al 2008a andTunc et al 2008;Frydman et al 2008;Avendañ o et al 2009a and2009b Labelling Direct labeling f O'Flaherty et al 2008;Zhang et al 2008;Aoki et al 2006;Torregrosa et al 2006;Young et al 2003;Muratori et al 2008a;.Greco et al 2005;Paasch et al 2004;Brugnon et al 2006;Donnelly et al 2000 Indirect labeling g Varum et al 2007;Sergerie et al 2005;...…”

Section: Comparing Flow Cytometry and Fluorescence Microscopy For Spementioning

confidence: 99%

Critical Aspects of Detection of Sperm DNA Fragmentation by Tunel/Flow Cytometry

Muratori

Tamburrino

Marchiani

et al. 2010

Systems Biology in Reproductive Medicine

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Despite the many studies documenting an increase of sperm DNA damage in subfertile and infertile subjects, determining whether this parameter is relevant for the clinic is still an open question. Indeed, results of clinical investigations on sperm DNA damage and outcome of ART procedures are often conflicting as many factors affect the predictive power of these tests. The techniques used to reveal such damage is one of these factors. Techniques detecting sperm DNA damage are indeed numerous and heterogeneous. In addition, it is not obvious that they reveal the same type of DNA damage and that their results are equivalent. One of the available methods to detect sperm DNA damage is the TUNEL assay. Since it was developed in the 1990s the TUNEL assay has been widely employed, becoming one of the most popular strategies to investigate the origin, the mechanism, and the clinical meaning of sperm DNA damage. Our group has used TUNEL coupled to flow cytometry to investigate sperm DNA fragmentation for about 10 years. According to our experience, this technique presents some pitfalls and limitations in the accuracy and reproducibility of the measures of sperm DNA fragmentation. In this review, we discuss several technical features of TUNEL and report the solutions adopted by our group to overcome some of its limitations.

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“…All procedures were performed in a laboratory dedicated to the handling of semen samples from patients known to be HIV-1 sero-positive (Muciaccia et al, 2007), in a Class II biological safety cabinet (Labotec, South Africa, Midrand, Gauteng). Standard macroscopic and microscopic semen analyses were performed on all samples according to the World Health…”

Section: Evaluation Of Neat Semen Samplesmentioning

confidence: 99%

Semen decontamination for the elimination of seminal HIV-1

Fourie

Loskutoff²,

Huyser

2015

Reproductive BioMedicine Online

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The risk of HIV transmission to the female partner, or potential offspring of an HIV-1 infected male can be reduced using semen decontamination procedures prior to assisted reproductive treatment (ART). The objective of this study was to determine the efficiency to decontaminate semen samples (N=186) from 95 HIV-1 sero-positive patients. Aliquots of neat semen were submitted for viral validation by qualitative and quantitative polymerase chain reaction. Semen samples were processed by density gradient centrifugation in combination with a ProInsert™ tube where after aliquots of the processed sperm samples were analysed for the presence of HIV-1. Fifty four percent of all tested neat semen samples tested positive for HIV-1 DNA, RNA or both (13.4%, 11.3% and 29.0%, respectively). From a total of 103 processed sperm samples that were submitted for viral validation, two samples tested positive for HIV-1 DNA and none for RNA. In conclusion, semen processing with the ProInsert™ followed by viral validation of processed sperm samples should be performed when providing assisted reproductive treatment to couples where the male partner is HIV-1 seropositive.

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HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

Abstract: We report molecular evidence demonstrating that HIV-1 infected subjects can ejaculate small amounts of HIV-1 DNA-positive abnormal spermatozoa. Their possible role in HIV-1 sexual transmission remains to be clarified.

Cited by 55 publications

References 85 publications

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Critical Aspects of Detection of Sperm DNA Fragmentation by Tunel/Flow Cytometry

Semen decontamination for the elimination of seminal HIV-1

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