HIV-1 viral load measurement in venous blood and fingerprick blood using Abbott RealTi m e HIV-1 DBS assay

Tang, Ning; Pahalawatta, Vihanga; Frank, Andrea M.; Bagley, Zowie; Viana, Raquel; Lampinen, John; Leckie, Gregor; Huang, Shihai; Abravaya, Klara; Wallis, Carole L.

doi:10.1016/j.jcv.2017.05.002

Cited by 35 publications

(39 citation statements)

References 7 publications

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“…We assessed the sensitivity of HIV RNA detection in DBS using the cut-off at 1,000 copies/ mL of plasma, the usual cut-off to consider for treatment failure. We found similarly to studies conducted in other settings [5,16,23] a very high sensitivity of Abbott system to detect HIV Table 1. Baseline characteristics of study population.…”

Section: Discussionsupporting

confidence: 86%

“…The higher level in plasma may also be due to the lack of hematocrit adjustment as reported in other studies [19,20]. This slightly lower average HIV RNA levels in DBS than in plasma has also been observed in other studies with average differences ranging between 0.07-0.76 log 10 copies/mL [5,12,16,21,22]. The smaller volume of blood tested in DBS might also explain that 2 DBS were undetectable while the corresponding plasma specimens had low HIV RNA VL (2.18 and 2.19 log 10 cp/mL) which were below the threshold of detection in DBS.…”

Section: Discussionsupporting

confidence: 77%

“…Due to the low number of specimens (n = 4) with viral load below 1,000 copies/mL in plasma, the specificity was 75% with a wide confidence interval. In other studies conducted on high numbers of DBS with low HIV VL, specificity of Abbott system ranged between 95-96% [5,16,23].…”

Section: Baseline Characteristics (N = 80)mentioning

confidence: 91%

“…Indeed, DBS present several advantages which make their use particularly attractive whenever access to VL testing is difficult: it is simple to prepare and convenient for blood collection and transportation (shipping by mail to a central testing laboratory) with low cost, and small volume of blood is needed. In addition, fingerpick blood specimen can be applied onto the DBS [5].…”

Section: Introductionmentioning

confidence: 99%

“…Routine monitoring of HIV RNA VL using DBS has been successfully implemented in resource-limited settings such as Angola, Tanzania, and Cameroon [10][11][12]. Several studies have evaluated the performances of the Abbott m2000 platform for the measurement of RNA in DBS versus plasma [5,[13][14][15][16]. Those studies were conducted among different populations (naïve or on ART) and used different DBS processing protocols.…”

Section: Introductionmentioning

confidence: 99%

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HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

Khamduang

Kaewbundit²,

Duangmano³

et al. 2020

PLoS ONE

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World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log 10 copies/mL. Using the 1,000 copies/mL cutoff , the sensitivity of DBS was 97% (95%CI, 91-100%) and specificity was 75% (95%CI, 19-99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 77%

Section: Baseline Characteristics (N = 80)mentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

Khamduang

Kaewbundit²,

Duangmano³

et al. 2020

PLoS ONE

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Feasibility of using dried blood spots for HIV viral load testing among HIV‐infected individuals in Thailand using QIAGEN QIAsymphony‐artus HIV‐1 platform

Thepbundit

Ngo‐Giang‐Huong

Salvadori

et al. 2019

Journal of Medical Virology

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In settings where plasma preparation and sample centralization are not feasible or inconvenient, dried blood spots (DBS) could be used as an alternative specimen to plasma to assess antiretroviral treatment response among HIV-infected individuals.This study was aimed to (1) validate the recent QIAsymphony-artus assay for DBS HIV viral load (VL) and (2) assess the feasibility of measuring HIV VL on DBS using this assay in Thailand. Ethylenediaminetetraacetic acid-blood samples from 99 HIVinfected individuals were used to prepare paired DBS and plasma. Also, DBS samples were shipped to three distant hospitals in the northern region. After short-term storage, DBS were returned by regular post to the AMS laboratory and were retested for HIV VL using the same platform. HIV VL results were compared using Pearson's correlation and Bland-Altman analysis. DBS HIV VL fairly correlated to plasma HIV VL (R = 0.62) with a mean difference of 0.02 log 10 IU/mL (SD = 1.06). A high correlation (R = 0.79) was observed between HIV VL in DBS before and after shipping (mean difference = 0.14 log 10 IU/mL, SD = 0.74), indicating good stability of HIV RNA in DBS. DBS can be used as an alternative specimen for HIV VL monitoring in Thailand. However, measurement of HIV VL with the QIAGEN QIAsymphony-artus assay should be improved, especially the DBS pre-extraction process. K E Y W O R D S DBS, HIV RNA load, HIV RNA measurement, HIV RNA quantification, performance, plasma viral load The research was performed at

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Examining Concordance Between Self-Report and Biomedical HIV Viral Load Data: A Scoping Review

et al. 2023

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HIV-1 viral load measurement in venous blood and fingerprick blood using Abbott RealTi m e HIV-1 DBS assay

Cited by 35 publications

References 7 publications

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

Feasibility of using dried blood spots for HIV viral load testing among HIV‐infected individuals in Thailand using QIAGEN QIAsymphony‐artus HIV‐1 platform

Examining Concordance Between Self-Report and Biomedical HIV Viral Load Data: A Scoping Review

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