HIV variants and hepatitis B surface antigen mutants: Diagnostic challenges for immunoassays

Schochetman, Gerald; Kuhns, Mary C.

doi:10.1002/jmv.20598

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…HBV has a high mutation rate due to its unique features [Schochetman and Kuhns, 2006], and such mutations can greatly hamper the sensitivity of immunoassays and molecular detection [Veropalumbo et al, 2010]. Presurface and surface (pre-S/S) gene mutations create a problem in the diagnosis, treatment, and post-assessment of hepatitis B [Weber, 2006;Sheldon and Soriano, 2008].…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the hepatitis B virus presurface and surface gene in patients from eastern China with occult hepatitis B

Huang

Qin

et al. 2013

Journal of Medical Virology

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This study was designed to detect and analyze mutations that occur within the presurface and surface (pre-S/S) gene of HBV in patients with occult hepatitis B, and determine their relationship to that disorder. Among 254 HBsAg negative samples of blood collected in eastern China, 183 were positive for anti-HBc alone, 61 were positive for anti-HBe alone, and 10 samples were positive for HBeAg. Within this group, 15 samples were found to be HBV DNA positive by real-time PCR and were designated Group I. A control group of 28 HBsAg positive samples were chosen at random from patients with chronic hepatitis B and designated Group II. The HBV pre-S/S gene was amplified by PCR and subjected to sequencing analysis. Occult hepatitis B was found in 1.6% of the patients with anti-HBc alone and in 3.3% of those with anti-HBe alone. Occult hepatitis B also was found in all HBsAg negative but HBeAg positive samples. Sequencing analysis showed a significant correlation between point mutations within the "a" determinant and occult hepatitis B (P < 0.0001), and a close relationship between pre-S deletion mutations and occult hepatitis B (P = 0.06). There were unique amino acid mutations at the G145 position other than G145R. The HBV DNA levels in patients with occult hepatitis B were significantly lower than those found in the control group. The "a" determinant mutations and pre-S deletions may play important roles in occult hepatitis B by affecting the expression, synthesis and secretion of the S protein and by impeding viral release and replication.

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Section: Introductionmentioning

confidence: 99%

Molecular analysis of the hepatitis B virus presurface and surface gene in patients from eastern China with occult hepatitis B

Huang

Qin

et al. 2013

Journal of Medical Virology

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“…Several unique features of HBV are associated with higher mutation rates [Schochetman and Kuhns, 2006]. HBV mutants often mix with wild-type viruses in the HBV carrier.…”

Section: Introductionmentioning

confidence: 99%

PreS deletion mutations of hepatitis B virus in chronically infected patients with simultaneous seropositivity for hepatitis‐B surface antigen and anti‐HBS antibodies

Huang

Qin

Zhang

et al. 2009

Journal of Medical Virology

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Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg and anti-HBs (Group I) and those of another 28 HBsAg positive but anti-HBs negative patients (Group II) were collected from CHB patients. Direct sequencing of polymerase chain reaction products or sequencing of clones was applied to both groups to determine sequences of HBV preS and S genes. Genotyping of the S gene indicated that all sampled HBVs were either Genosubtype Ba or Genosubtype Ce. Seven samples in Group I harbored HBV preS deletion mutations. Three of the seven samples showed large deletion mutations in 3' terminus of preS1 and co-existence of the mutant type and the full-length wild type, and the remaining four samples showed deletion mutations in 5' terminus of preS2. All mutant strains were found to be genosubtype Ce. Only two samples in Group I showed G145R/A mutation. Only one sample in Group II contained preS deletion mutation. It is therefore concluded that HBV preS deletion mutations are likely to be related to the coexistence of HBsAg and anti-HBs in CHB patients (P-value = 0.024). Some immune reactions may select for the preS deletion in CHB patients with anti-HBs, the possible marker for immune selection.

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“…A similar situation can be imagined for HBV chronic carriers receiving liver transplantation supported by HBiG. Indeed, there are now several reports of the surprising prevalence of HBV "a" antigen mutants, which would be expected to refractory to current "a" epitope dependent vaccines [90]. In one report, the incidence of HBsAg mutants in infants born from HBV carrier mothers and treated with vaccine and HBiG was 4% [43].…”

Section: Immunizationmentioning

confidence: 69%

Molecular Virology of Hepatitis B Virus for Clinicians

Block

Guo

2007

Clinics in Liver Disease

153

128

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SynopsisThis chapter reviews the molecular biology of the hepatitis B virus (HBV) in an effort to explain its natural history from a molecular perspective. The life cycle of the virus, with special attention to virus replication, polypeptide production and morphogenesis, is described. The way in which these steps may influence the natural history of viral pathogenesis, as well as the effectiveness of interventions, receives special consideration.

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HIV variants and hepatitis B surface antigen mutants: Diagnostic challenges for immunoassays

Cited by 8 publications

References 15 publications

Molecular analysis of the hepatitis B virus presurface and surface gene in patients from eastern China with occult hepatitis B

Molecular analysis of the hepatitis B virus presurface and surface gene in patients from eastern China with occult hepatitis B

PreS deletion mutations of hepatitis B virus in chronically infected patients with simultaneous seropositivity for hepatitis‐B surface antigen and anti‐HBS antibodies

Molecular Virology of Hepatitis B Virus for Clinicians

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