Hl-a Antigen Studies With Leukocyte Cell Lines

Production of β2‐Microglobulin by Normal and Malignant Human Cell Lines and Peripheral Lymphocytes

“…Such lines have also been employed for the production of HL-A (Mann et al 1969, Reisfeld et al 1970. LCL have essentially normal diploid karyotypes (Huang & Moore 1969, L. Zech, personal communication) and are very stable in phenotypic expressions like immunoglobulin synthesis (Finegold et al 1967, Wakefield et al 1967, Matsuoka et al 1968, Nilsson 1971b), HL-A antigens (McDonald et al 1970, Reisfeld et al 1970, Lindblom & Nilsson 1973), G6-PD and PGM isoenzymes (Conover et al 1970, Fialkow, personal communication) and they are therefore suited for controlled quantitative studies of (^2-1.1 production. The main reason, however, why LCL were the first cell lines in vitro to become examined for y?2-// secretion was their capacity to synthesize immunoglobulin.…”

Epstein-Barr Virus Infection in Relation to Infectious Mononucleosis and Burkitt's Lymphoma

“…High levels of stimulation were observed whether the combination was au toch thonous or allogeneic, but in nearly every combination examined allo geneic pairs showed significantly greater stimulation than autochthonous pairs. This high level of autochthonous recognition is not due to changes in HL-A mosaic, for these are unchanged in LCL (90,91). Stimulation does not appear due to absorption of culture medium ingredients by the cell lines.…”

“…Interferon(84)(85)(86), migration inhibitory factor (87), lymphotoxin (87), inhibitors of DNA synthesis(88), mitogenic factors (88), HL-A antigens in large quantity(89)(90)(91), and intact immunoglobulins (92-103) have been described in the culture fluids in which these cell lines arc grown. The amount of immunoglobulin secreted by most cells is small, 15 mg per 109 cells per 24 hr in one study.…”