HL-A Antigens and Genes - III. Production of HL-A Typing Antisera of Desired Specificity

Abstract

Eight skin graft donor-recipient pairs were selected by lymphocyte typing for production of HL-A typing antisera. After the second or third skin graft from the same donor, antibodies appeared in the sera of five of the recipients. The antibodies were directed against antigens which seem to be determined by alleles of the LA and 4 sub-loci, and the specificity showed close correlation to the HL-A incompatibility between donor and recipient. By re-immunization with intradermal leucocyte injections, two additional recipients produced antibodies against donor cells. One of these antisera seemed to detect a new HL-A antigen of the 4 sub-locus (called FJH). It is concluded that skin grafting and leucocyte injections is a simple and efficient method for production of HL-A typing antisera of desired specificity. The results also indicate that the remaining alleles of the HL-A system may be identified by antisera obtained by this method.

New HL-A Alleles

“…In addition, the donor must have one or two unknown LA antigens (x). After three skin grafts from the donor, anti-HL-A5 antibodies appeared in the serum of the recipient, as previously reported [15,16].…”

“…A major incompatibility with respect to unidentified HL-A antigens may then exist. In this way antigen FJH was identi fied, most probably determined by a new allele at the Four sub-locus [16]. The LA antigen HL-A9 (Lcll, LA4, TH -BS) was identified by Walford et al in a similar way [21].…”

“…Each series of antigens seems to be determined by multiple mutually exclusive genes which behave as alleles in population and family studies [2,5,6,13,14,16]. The two loci or sub-loci within the HL-A chromosomal region are closely linked; a maximum recombination frequency of less than 0.53% has been calculated [13].In a recent paper we reported how the remaining alleles of the HL-A system may be identified with antisera obtained by immuniza tion within selected donor-recipient pairs [16]. The recipients should then preferably contain two well-defined antigens of both sub-loci.…”

“…Each series of antigens seems to be determined by multiple mutually exclusive genes which behave as alleles in population and family studies [2,5,6,13,14,16]. The two loci or sub-loci within the HL-A chromosomal region are closely linked; a maximum recombination frequency of less than 0.53% has been calculated [13].…”

Complement-Fixing Platelet Iso-Antibodies V. HL-A Typing

“…Typing reagents were obtained by screening thousands of sera from parous women, transfused patients or individuals immunized by planned skin grafting and leukocyte injections [17]. The sera were treated aseptically and stored at -20°C or -75°C or at 4°C after lyophilization.…”