HLA‐B*1586 is a novel, hybrid HLA‐B15/B22 allele with unique serology and haplotypic association

Recognition of the HLA‐B*15:86 allele and its associated HLA haplotype in a Taiwanese individual

“…We were unable to perform a family study to deduce the HLA haplotype in association with B*15:86 because of the lack of specimens from the family members of the individual with the novel allele. However, comparing the HLA typing of the individual identified by us with that of cell (VTIS123353) that had previously been reported to the IPD‐IMGT/HLA Database 1 and the haplotype of the family reported by Yang et al, 2 we propose that B*15:86 is possibly on the haplotype A*11:01‐C*01:02‐B*15:86 . In addition, we further propose that the haplotypes of our donor with B*15:86 are A*11:01‐C*01:02‐B*15:86‐DRB1*15:01‐DQB1*06:01 and A*11:01‐C*08:01‐B*15:02‐DRB1*15:01‐DQB1*06:01 since the individual is homozygous for DRB1*15:01 and DQB1*06:01 .…”

Identification of a New HLA-B Allele (B*1576) by Haplotype Specific Extraction

“…Haplotype specific extraction provides a rapid, automated method for determining true (i.e. non-statistically derived) and previously unknown haplotypes, directly from genomic DNA, without requiring any familial information [4][5][6][7][8][9][10]. The method does not rely on amplification-based phasing methods which can be affected by polymorphic variation and eliminates the need for cloning or subcloning for the characterization of new alleles.…”

Analysis of the complete genomic sequence of HLA‐A alleles in the Chinese Han population

“…Nonsilent point mutations (Parham et al, 1995) and gene conversion events (Madrigal et al, 1993) (A*4301 that is identical to A*2601 in all positions except for codons 62 and 63, which are identical to those of the A*2901 allele) have been reported as possible predominant mechanisms involved in the generation of the HLA polymorphism. In addition, other studies indicated that certain HLA Class I alleles could have been generated from intra loci single recombination events in intron 2 as the B*1522 allele (Martinez-Laso et al, 1995; the B*1522 allele has now been renamed as B*3543), gene conversion with participation of an intron 2 ⁄ exon 3 segment as B*3906 allele (Vargas-Alarco¢n et al, 1997) and allele as HLA-B*1586 that comes from hybrid HLA-B15 ⁄ B22 alleles (Yang et al, 2006).…”

“…, 1995; the B*1522 allele has now been renamed as B*3543), gene conversion with participation of an intron 2/exon 3 segment as B*3906 allele (Vargas‐Alarco′n et al. , 1997) and allele as HLA‐B*1586 that comes from hybrid HLA‐B15/B22 alleles (Yang et al. , 2006).…”