HLA-linked B cell alloantigens of a new segregant series: Population and family studies of the SB antigens

Shaw, Stephen; Pollack, Marilyn S.; Payne, Susan N.; Johnson, Armead H.

doi:10.1016/0198-8859(80)90104-4

Cited by 78 publications

(24 citation statements)

References 18 publications

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“…Polymorphism of the DP subregion products has until recently been analyzed by primed lymphocyte typing (PLT), and there are eight defined allotypes, designated HLA-DPwl-6 (Shaw et al 1980), Cp63 (Nose et al 1985), and CPD-6EI (Odum et al 1986). Despite considerable efforts to DP type Caucasoids using PLT (Shaw et al 1981, Pawelec et al 1982, Moen et al 1983, Wank and Schendel 1984, Morling et al 1984, the lack of alternative methods is probably responsible for the paucity of information on DP allele frequencies in non-Caucasoid populations, or within disease groups.…”

Section: Introductionmentioning

confidence: 99%

Southern blot analysis of HLA-DP gene polymorphisms in Caucasoid rheumatoid arthritis (RA) patients and controls

et al. 1989

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Despite extensive analysis of the incidence of HLA-DR and HLA-DQ allele frequencies in defined autoimmune disease groups, there is very little information available on HLA-DP allele frequencies. This is largely because HLA-DP typing has until recently been restricted to primed lymphocyte typing (PLT). However, allelic polymorphism of the HLA-DP subregion can now be studied by Southern blot analysis or genotyping with DPA1 and DPB1 probes. By direct counting of allele-specific DNA fragments, we have analyzed the frequencies of five major DP genotypes (DPw1, DPw2, DPw3/6, DPw4, and DPw5), in a large number of Caucasoid rheumatoid arthritis (RA) patients (n = 74), and controls (n = 91). The predicted frequency of DP alleles in both patient and control groups was comparable to PLT-determined DP allele frequencies in normal Caucasoids. However, the gene frequency of DPw4 was increased in the RA patients, with 51% of the patients studied scoring as DPw4, 4 homozygotes. With the exception of one possible combination (DPw5 and DRw6) in the controls, no significant linkage disequilibrium was detected between DP and DR alleles in either patient or control groups. Thus the prevalence of DPw4 in the RA patients is independent of any disease association with the DR loci, and may represent a new class II association with RA.

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Section: Introductionmentioning

confidence: 99%

Southern blot analysis of HLA-DP gene polymorphisms in Caucasoid rheumatoid arthritis (RA) patients and controls

et al. 1989

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“…Previous studies on the HLA-D region have shown that the various class I1 gene products might have similar functions. Both HLA-DR and DP antigens have been described as restriction elements in the lyses of haptenated or virus infected target cells (Bergholz & Thorsby 1977, Hansen et al 1978, Lamb et al 1982, Eckels et al 1983) and DP-as DR-antigens have been shown capable of simulating the proliferative and cytotoxic responses (Mawas et al 1978, Shaw et al 1980, Ohta et al 1984.…”

Section: Discussionmentioning

confidence: 99%

HLA‐DP related suppression of mixed lymphocyte reaction with alloactivated lymphocytes

et al. 1986

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We studied the influence of HLA class I and class II antigens on the suppression of the MLR induced by primed lymphocytes (PLs) alloactivated in vitro. The suppression of 14 different PLs of 83 MLRs was analyzed. The PLs were primed against (i) HLA‐DP (SB) (ii) HLA‐DR/DQ or (iii) both HLA‐DP and DR/ DQ. The suppression was analyzed with special reference to the sharing of HLA‐antigens between (i) the stimulator in the MLR and (ii) the stimulator generating the PL. HLA‐DP and HLA‐DR/DQ antigens were equally capable of generating suppressor cells. When these cells were added to MLRs, the specific stimulators induced the strongest suppression (74%), while allogeic cells sharing class II antigens induced a slightly weaker suppression (66%). The suppression related to HLA‐DP (60%) was almost identical to that related to HLA‐DR/DQ (59%). The HLA‐A, B, C related suppression was of the same magnitude (58%). The unspecific suppression (40%), i.e. no relation to known HLA‐antigens, was significantly lower than the class II related suppression, but not significantly lower than the class I related suppression. The suppression of the MLR did not seem to be caused by cytotoxic cells, consumption of lymphokines, nor changes in the kinetics of the MLR. Thus, HLA‐DP antigens can ‐ like DR/DQ antigens ‐ induce PLs with the ability to suppress the MLR in an HLA‐class II (DP or DR/DQ) related, and possibly a class I related, as well as an unspecific fashion.

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“…Also, Table 1 illustrates that most serologically defined DR and DQ antigens have uniquely distinct eplets. The cellularly defined DP specificities [originally called SB (9)] do not have unique eplets, and this explains why they cannot be readily determined serologically with monospecific sera.…”

Section: Epitopes and A Historical Perspective Of Serological Hla Typingmentioning

confidence: 99%

Reflections on HLA Epitope-Based Matching for Transplantation

Duquesnoy

2016

Front. Immunol.

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HLA antibodies are primary causes of transplant rejection; they recognize epitopes that can be structurally defined by eplets. There are many reviews about HLA epitope-based matching in transplantation. This article describes some personal reflections about epitopes including a historical perspective of HLA typing at the antigen and allele levels, the repertoires of antibody-verified HLA epitopes, the use of HLAMatchmaker in determining the specificities of antibodies tested in different assays, and, finally, possible strategies to control HLA antibody responses.

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HLA-linked B cell alloantigens of a new segregant series: Population and family studies of the SB antigens

Cited by 78 publications

References 18 publications

Southern blot analysis of HLA-DP gene polymorphisms in Caucasoid rheumatoid arthritis (RA) patients and controls

Southern blot analysis of HLA-DP gene polymorphisms in Caucasoid rheumatoid arthritis (RA) patients and controls

HLA‐DP related suppression of mixed lymphocyte reaction with alloactivated lymphocytes

Reflections on HLA Epitope-Based Matching for Transplantation

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