Hla-Restricted Virus-Specific Cytotoxic T-Lymphocyte Responses to Live and Inactivated Influenza Vaccines

Ennis, F A; Qi, Yihua; Riley, Diana; Rook, A H; Schild, G. C.; Pratt, Robert; Potter, C. W.

doi:10.1016/s0140-6736(81)91389-1

Cited by 94 publications

(58 citation statements)

References 18 publications

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“…It was previously shown that CTL are detectable in blood after 6-14 days and reach peak level at day 14 than return to baseline 12 months after influenza vaccination [4] which is consistent with our results. We found that the frequency of two monitored populations of influenza-specific CTL (HA [344][345][346][347][348][349][350][351][352][353] , and HA 541-549 ) significantly increased 7 days after vaccination.…”

Section: Discussionsupporting

confidence: 93%

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

et al. 2007

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With emergence of MHC class I tetramers loaded with CD8+ T‐cell viral epitopes, it is possible to study virus‐specific CD8 cells in humans during infection and after vaccination. MHC class I tetramers was used to detect the frequency of haemagglutinin (HA)‐specific T cells in 26 healthy influenza‐vaccinated humans. Peripheral blood was collected before, and 7, 14 and 28 days after vaccination. Four‐colour flow cytometry was used for monitoring of vaccine induced T‐cell response. In 15 donors, two‐ to fivefold increase in frequency of HA‐specific T cells was observed 7 days after vaccination. In addition, in 12 of these donors, this increase was accompanied with fourfold increase of H1N1 antibody titre. The increase in frequency of HA‐specific CD8+/IFN‐γ+ cells was low and peaked 28 days after vaccination in three of the six donors tested. Frequencies of HA‐specific CD8+ T cells and antibody titre returned to prevaccination values 1 year after vaccination. Subunit influenza vaccines have the ability to induce HA‐specific CD8+ cells. As the immune response to this vaccine decreased significantly after 1 year, our results confirm the importance of annual immunization for adequate protection.

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Section: Discussionsupporting

confidence: 93%

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

et al. 2007

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“…An increase in cytotoxic lymphocytes (CTL) responses in PBMCs after IVV has been reported but these responses have been low and transient (18,19). CTL response were sought in the present study and detected in an occasional volunteer but differences between the two vaccines for different antigen stimulations at the different test times were not seen.…”

Section: Discussionmentioning

confidence: 99%

Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvant

Mbawuike¹,

Zang²,

Couch³

2007

Vaccine

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To evaluate humoral (antibody) and cell mediated immune (CMI) responses, 30 healthy young adults were either given inactivated influenza vaccine with or without QS21 adjuvant. Vaccination site pain and postvaccination myalgias were greater in the QS21 group. Serum antibody increases occurred in 73 to 93% of subjects for each vaccine and antigen at 2 weeks and 4 weeks but frequencies and mean titers for the two vaccines were not different. No differences in T cell cytotoxicity were detected for either vaccine for influenza A or B infected cells. IFN-γ for both vaccine groups was increased in supernates after 3 days but not 7 days of stimulation in the cytotoxicity tests; amounts for the two vaccines were similar. To further evaluate CMI, remaining PBMCs were stimulated overnight with cells infected with each vaccine strain; an increase in spot forming cells (sfc) for Granzyme B and IFN-γ was found for all subjects and in 51 of 54 sfc tests. A slightly higher response in the Gran B test for QS21 subjects was suggested, but no clear immune response advantage was identified among healthy adults for QS21 adjuvanted influenza vaccine.

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“…Knowledge about human CD8 T cell immune responses has been more well developed than human CD4 T cell responses [17,18]. Studies on the cytotoxic T lymphocyte repertoire to influenza A viruses indicate that influenza memory T cell responses are directed to a number of epitopes on a variety of proteins including the nucleoprotein, nonstructural protein 1 (NS1), and the matrix protein 1(M1) [19,20].…”

Section: Introductionmentioning

confidence: 99%

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine

Co¹,

Orphin²,

Cruz³

et al. 2008

Vaccine

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Thirty adults were tested for humoral and cellular immune responses following immunization with the trivalent inactivated influenza vaccine. Modest but significant inverse correlations between the baseline and the fold changes in the number of IFNγ producing cells and the levels of neutralizing antibodies were observed. Specific increases in proliferative responses in the CD8 CD45RA+ population were noted after vaccination. Minimal correlations between neutralizing antibody titers and the number of IFNγ producing cells in terms of prevaccination levels or fold increases were observed. These results show specific increases in a CD8 T cell subset and discordant T and B responses induced by the inactivated influenza vaccine.

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Hla-Restricted Virus-Specific Cytotoxic T-Lymphocyte Responses to Live and Inactivated Influenza Vaccines

Cited by 94 publications

References 18 publications

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvant

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine

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Hla-Restricted Virus-Specific Cytotoxic T-Lymphocyte Responses to Live and Inactivated Influenza Vaccines

Cited by 94 publications

References 18 publications

Enumeration of Haemagglutinin‐specific CD8+ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

Enumeration of Haemagglutinin‐specific CD8+ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvant

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine

Contact Info

Product

Resources

About

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

Enumeration of Haemagglutinin‐specific CD8⁺ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers