HM-PAO-imaging and herpes encephalitis

Cleator, Graham M.; Lewis, A. G.; Klapper, Paul E.; Hl, Sharma; Smith, Anne Marie

doi:10.1007/bf01311086

Cited by 1 publication

(1 citation statement)

References 11 publications

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“…constitutively over-transcribed CYP6A2, and previous work with northern and western blots has demonstrated that CYP6G1 and CYP12D1 proteins are over-expressed in Wisconsin [58]. The constitutive over-expression of any one or a combination of P450s may contribute to the Wisconsin strain being more susceptible than the Canton-S strain to H 2 O 2 because at least some P450s generate reactive oxygen species (ROS) as a by-product of catalysis [72][73][74][75], thereby increasing cellular oxidative stress. Oxidative stress is known to have negative impact on biological systems [76,77].…”

Section: Discussionmentioning

confidence: 99%

Differential transcription of cytochrome P450s and glutathione S transferases in DDT-susceptible and -resistant Drosophila melanogaster strains in response to DDT and oxidative stress

Sun

Schemerhorn

Jannasch

et al. 2011

Pesticide Biochemistry and Physiology

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Metabolic DDT resistance in Drosophila melanogaster has previously been associated with constitutive over-transcription of cytochrome P450s. Increased P450 activity has also been associated with increased oxidative stress. In contrast, over-transcription of glutathione S transferases (GSTs) has been associated with resistance to oxidative stress. However, little is known in regards to the impact of xenobiotics on induction of P450s and GSTs and if there exist differences in inducibility between the pesticide susceptible and resistant strains. Thus, we investigated the transcriptional expression of GSTs and P450s in DDT resistant (Wisconsin) and susceptible (Canton-S) Drosophila strains in response to exposure to DDT and the oxidative stressor H 2 O 2. Wisconsin constitutively over-transcribed P450s, constitutively under-transcribed 27% of its total GSTs, and was more susceptible to H 2 O 2 than CantonS. DDT exposure induced GST expression only in the Wisconsin strain and not in the CantonS strain. These results suggest that there are potentially more differences between pesticide susceptible and resistant strains than just constitutive expression of P450s; there may also exist, at least in some strains, differences in their patterns of inducibility of P450s and GSTs. Within the context of the Wisconsin strain, these differences may be contributing to the fly lines increased susceptibility to oxidative stress.

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